Analysis of the mechanism of polarity establishment on the plasma membrane for polar secretion in Plants.
| Project/Area Number |
16H05068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
Sato Masa H. 京都府立大学, 生命環境科学研究科, 教授 (20283575)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | ホスホイノシチド / 極性確率 / 膜交通 / 根毛形成 / シロイヌナズナ / FAB1 / 極性輸送 / 根毛 / イノシトールリン脂質 / 極性確立 / 細胞極性確立 / イノシトールリン脂質シグナリング / 細胞壁 / 細胞骨格 / 微小管 / 表層微小管 / 分泌経路 / 極性分泌 / 細胞内小胞輸送 / 極性確立メカニズム |
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| Outline of Final Research Achievements |
Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. Here we show that PI(3,5)P2, the enzymatic product of FAB1, is involved in the hardening of the shank in root hairs in Arabidopsis. FAB1 and PI(3,5)P2 localize to the plasma membrane along the shank of growing root hairs. By contrast phosphatidylinositol 4-phosphate 5-kinases 3 (PIP5K3) and PI(4,5)P2 localize to the apex of root hair where they are required for tip growth. Reduction of FAB1 function results in the formation of wavy root hairs while wild type are straight. The localization of FAB1 in the plasma membrane of root hair shank requires the activity of Rho-related GTPases from plants 10 (ROP10) and localization of ROP10 requires FAB1 activity. Computational modelling of root hair morphogenesis successfully reproduces the wavy root hair phenotype. Taken together, these data demonstrate that the root hair shank hardening requires PI(3,5)P2/ROP10 signaling.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は，申請者が発見した二種類のイノシトールリン脂質の細胞膜上の偏在性に注目し，極性分泌制御の観点から植物細胞の形態形成の基本原理を明らかにしようとする挑戦的な研究である。植物にとっての極性分泌現象は，クチクラや根における金属キレート物質の分泌，蜜腺での蜜の分泌，ワックスの分泌などにも必須の現象である。今回明らかになったFAB１およびPI(3,5)P2による細胞の極性分泌メカニズムの理解は，応用的には，農作物や花きなどの形の制御による品種改良，細胞壁やクチクラ，ワックス層の増強による病害性抵抗性増強などにつながる基盤技術になると考えている。
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Concerted action of evolutionarily ancient and novel SNARE complexes in flowering-plant cytokinesis2018
Author(s)
Minsoon Park, Cornelia Krause, Matthias Karnahl, Ilka Reichardt, Farid El Kasmi, Ulrike Mayer, York-Dieter Stierhof, Ulrike Hiller, Georg Strompen, Martin Bayer, Marika Kients, *Masa H. Sato*, Marc T. Nishimura, Jeffery L. Dangl, Anton A. Sanderfoot, Gerd Juergens
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Journal Title
Dev. Cell
Volume: 44
Issue: 4
Pages: 500-511
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3.2017
Author(s)
Nagel MK, Kalinowska K, Vogel K, Reynolds GD, Wu Z, Anzenberger F, Ichikawa M, Tsutsumi C, *_Sato MH_*, Kuster B, Bednarek SY, Isono E.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 114
Issue: 34
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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