| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
Epithelial cells are the largest number of cells in the body that cover the internal and external surfaces of multicellular organisms. They are responsible for the functions and structures of major organs such as the skin, liver, and kidneys. The TJ (Tight Junction), composed of the polymer of claudin (Cldn) family protein molecules, reside in between epithelial cells. The paracellular permeability is mainly determined to maintain the tissue microenvironment properly. Biological functions are carried out within the exactly regulated tissue microenvironment. The failure of it is linked to various pathological conditions directly. In this application, we generate the Cldn null epithelial cells by using the CRISPR/CAS9 system to advance the functional analysis of Cldn-based TJ.
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