| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
In this study, we attempted direct observations for dynamics of membrane protein complex in the membrane rafts using using a high-speed atomic force microscopy (HS-AFM).
First we succeed to establish a method for purifying functional membrane proteins, e.g. Cystic Fibrosis Transmembrane conductance Regulator (CFTR) and Aquaporin 4 (AQP4), and reconstituting them in a lipid bilayer. First we observed single molecular dynamics of CFTR and succeeded to identify large fluctuation of presumable NBD and R-domain of CFTR protein. Next we successfully visualized the single molecule dynamics of the anti-AQP4 monoclonal antibodies interacting with AQP4 molecules forming a supramolecular orthogonal array structure reconstituted in lipid membrane, which initiates a severe immune-disease in human, Neuromyelitis Optica (NMO).
We also study disease-associated CFTR mutants causing a life-shorten disease Cystic Fibrosis, which should be important research targets in near future.
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