| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
We aim to understand the mechanisms of Systemic RNAi. We used a rsd-3 mutant strain as a parent, which is defective in RNAi, and screened for suppressor mutants. We found that a zip-type zinc transporter gene mutation results in the recovery of RNAi. A human homolog gene was able to rescue the mutant phenotype, suggesting specific and functional conservation.
We additionally screened for more RNAi mutants. We mutagenized animals and treated them with an RNAi clone, which kills wild-type animals. We isolated mutant strains which are resistant to the RNAi treatment. We identified mutant genes by whole genome sequencing. A small G regulatory protein and a nematode-specific protein are RNAi-resistant.
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