| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Outline of Final Research Achievements |
In this study, we investigated the molecular mechanism of neurodegeneration involving tRNA metabolism and the function of a novel RNA kinase molecule, NOL9. In vitro and in vivo analysis of various tRNA fragments, which have been reported to cause neuronal cell death, revealed that 5'Tyr-tRF derived from tyrosine tRNA induces p53-dependent neuronal cell death. In vitro kinase assays using the mouse fibroblasts or recombinant proteins produced by a cell-free protein expression system were performed for NOL9. However, no kinase activity was observed in NOL9 molecules, which was surprisingly different from previous reports.
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