• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of early tumorous lesion by atypical DNA methylation

Research Project

Project/Area Number 16H05161
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionEhime University

Principal Investigator

Kitazawa Sohei  愛媛大学, 医学系研究科, 教授 (90186239)

Co-Investigator(Kenkyū-buntansha) 北澤 理子  愛媛大学, 医学部附属病院, 准教授 (00273780)
原口 竜摩  愛媛大学, 医学系研究科, 准教授 (00423690)
Research Collaborator Yasukawa masaki  
Takenaka atsushi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
KeywordsDNAメチル化 / 組織細胞化学 / 癌細胞 / 老化 / ミトコンドリア / エピジェネティクス / MeCP2 / 破骨細胞 / 副甲状腺 / 骨転移 / 遺伝子改変動物 / 腎臓癌 / 酸化的ストレス / 糖尿病 / 腫瘍早期病変 / 非定型的メチル化 / エピゲノム不安定性 / 病理診断 / 癌 / メチル化 / 腫瘍 / 遺伝子発現 / 腫瘍関連遺伝子 / 初期病変
Outline of Final Research Achievements

Atypical DNA methylation, such as CpA, CpT, CpC (non-CpG-selective), or DNA methylation other than CpG-island, and DNA methylation in mitochondrial DNA is analyzed by use of the newly developed sequence-specific in situ demonstration of DNA methylation technique on gene expression mechanism under the influence of oxidative-stress and aging. Secondary neoplasia to dialysis or diabetic high oxidative stress and neuronal aging were the two major targets of this study. We discovered that atypical DNA methylation is increased with exposure to high oxidative stress and cell senescence. These findings open the gate for discovering of diagnostic cues to early stage of tumorigenesis.

Academic Significance and Societal Importance of the Research Achievements

これまで、非CpG-island領域のメチル化シトシンの病理病態に及ぼす影響に関しては、世界でもほとんど検討されておらず、申請者らの形態的特徴に基づく細胞集団の選別、微量検体からのメチル化解析手法、非CpG-island領域を対象とした研究により、癌の進展過程で観察される癌細胞の多様性を規定するエピジェネティクス制御機構の一旦が解明され、新たな治療戦略へと展開することが期待される。現在進行している癌のゲノム診療のさらに先を目指す診断、治療へのあたらな選択肢を提供するものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (32 results)

All 2018 2017 2016

All Journal Article (10 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (22 results)

  • [Journal Article] Novel GLI3 variant causing overlapped Greig cephalopolysyndactyly syndrome (GCPS) and Pallister-Hall syndrome (PHS) phenotype with agenesis of gallbladder and pancreas.2018

    • Author(s)
      Ito S, Kitazawa R, Haraguchi R, Kondo T, Ouchi A, Ueda Y, Kitazawa S.
    • Journal Title

      Diagn Pathol.

      Volume: 3;13(1):1 Issue: 1 Pages: 1-4

    • DOI

      10.1186/s13000-017-0682-8

    • NAID

      120006425668

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pathologic conditions of hard tissue: role of osteoclasts in osteolytic lesion.2018

    • Author(s)
      Kitazawa R, Haraguchi R, Fukushima M, Kitazawa S.
    • Journal Title

      Histochem Cell Biol

      Volume: 149(4) Issue: 4 Pages: 405-415

    • DOI

      10.1007/s00418-018-1639-z

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Growth plate-derived hedgehog-signal-responsive cells provide skeletal tissue components in growing bone.2018

    • Author(s)
      Haraguchi R, Kitazawa R, Imai Y, Kitazawa S.
    • Journal Title

      Histochem Cell Biol

      Volume: 149(4) Issue: 4 Pages: 365-373

    • DOI

      10.1007/s00418-018-1641-5

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Morphology-oriented epigenetic research.2018

    • Author(s)
      Kitazawa S, Haraguchi R, Kitazawa R.
    • Journal Title

      Histochem Cell Biol.

      Volume: 150(1) Issue: 1 Pages: 3-12

    • DOI

      10.1007/s00418-018-1675-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Histological assessment of the efficacy of drug-eluting beads in portal tumor thrombosis of hepatocellular carcinoma2017

    • Author(s)
      Yusuke Imai, Masashi Hirooka, Yohei Koizumi, Yoshiko Nakamura, Takao Watanabe, Osamu Yoshida, Yoshio Tokumoto, Eiji Takeshita, Masanori Abe, Hiroaki Tanaka, Mie Kurata, Sohei Kitazawa,Yoichi Hiasa
    • Journal Title

      Radiology Case Reports

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of characteristic features of pineal germinoma that enhance accuracy of preoperative differentiation in pineal region tumors: its significance on optimum surgical treatment.2017

    • Author(s)
      noue A, Ohnishi T, Kohno S, Ohue S, Iwata S, Matsumoto S, Nishikawa M, Ozaki S, Mizuno Y, Kitazawa R, Kunieda T.
    • Journal Title

      Neurosurg Rev

      Volume: in press Issue: 1 Pages: 197-206

    • DOI

      10.1007/s10143-017-0835-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Histological assessment of the efficacy of drug-eluting beads in portal tumor thrombosis of hepatocellular carcinoma.2016

    • Author(s)
      Imai Y, Hirooka M, Koizumi Y, Nakamura Y, Watanabe T, Yoshida O, Tokumoto Y, Takeshita E, Abe M, Tanaka H, Kurata M, Kitazawa S, Hiasa Y.
    • Journal Title

      Radiol Case Rep.

      Volume: 12 Issue: 1 Pages: 179-184

    • DOI

      10.1016/j.radcr.2016.11.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] sFRP4-dependent Wnt signal modulation is critical for bone remodeling during postnatal development and age-related bone loss.2016

    • Author(s)
      Haraguchi R, Kitazawa R, Mori K, Tachibana R, Kiyonari H, Imai Y, Abe T, Kitazawa S.
    • Journal Title

      Sci Rep.

      Volume: 6:25198 Issue: 1 Pages: 1-14

    • DOI

      10.1038/srep25198

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TDRG1 functions in testicular seminoma are dependent on the PI3K/Akt/mTOR signaling pathway2016

    • Author(s)
      Yong Wang, Yu Gan, Zhengyu Tan, Jun Zhou, Riko Kitazawa, Xianzhen Jiang, Yuxin Tang, and Jianfu Yang
    • Journal Title

      OncoTargets and Therapy

      Volume: 7 Issue: 7 Pages: 409-420

    • DOI

      10.1080/15384047.2016.1178425

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inflammatory Fibroid Polyps of Large Bowel with PDGFRA Mutation2016

    • Author(s)
      Kanazu Ariyasu, Riko Kitazawa, Ryuma Haraguchi, Yasuo Ueda, Yukino Kawanami, Yukiko Nishi, Yuri Kameoka, Yosuke Mizuno and Sohei Kitazawa
    • Journal Title

      J Cell Sci Ther

      Volume: 7 Issue: 01 Pages: 234-239

    • DOI

      10.4172/2157-7013.1000234

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 糖尿病性骨減少に抵抗性を示すsFRP-4遺伝子欠損マウスの病態組織学的考察2018

    • Author(s)
      伊吹 優里, 原口 竜摩, 北澤 理子, 今井 祐記, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 下部消化管糖尿病合併症におけるヘッジホッグシグナル経路の関与2018

    • Author(s)
      倉田 菜央, 原口 竜摩, 小野田 杏奈, 北澤 理子, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 中枢神経系におけるヘッジホッグシグナル依存的な糖尿病合併症2018

    • Author(s)
      池田 真子, 原口 竜摩, 北澤 理子, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 糖尿病により誘発される精子形成能低下とヘッジホッグシグナル経路との関連性について2018

    • Author(s)
      大野 輝之, 原口 竜摩, 齋藤 洋太, 下山 貴幸, 北澤 理子, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 成長板に由来するヘッジホッグシグナル受容細胞の骨格発生における役割2018

    • Author(s)
      原口 竜摩, 北澤 理子, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Calmodulin-like 5(CALML5)の子宮頸部扁平上皮癌における発現制御2018

    • Author(s)
      上田 康雄, 北澤 理子, 福島 万奈, 近藤 武史, 原口 竜摩, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの機能解析(Functional analysis of a novel splicing variant of receptor activator of NF-κB)2018

    • Author(s)
      北澤 理子, 原口 竜摩, 上田 康雄, 福島 万奈, 北澤 荘平
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ミトコンドリアDNA多型を用いた腫瘍細胞クローナリティー解析2018

    • Author(s)
      北澤 荘平, 神崎 摩耶, 原口 竜摩, 北澤 理子
    • Organizer
      日本病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 組織in situでのメチル化シトシン検出手法の開発2018

    • Author(s)
      矢野 可蓮, 原口 竜摩, 城戸 貴弘, 神崎 摩耶, 北澤 理子, 北澤 荘平
    • Organizer
      日本組織細胞化学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 組織化学イメージングで探る硬組織の細胞機能 ヘッジホッグシグナルを介する成長板を発生起点とした骨格形成の理解2018

    • Author(s)
      原口 竜摩, 北澤 理子, 北澤 荘平
    • Organizer
      日本組織細胞化学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 破骨細胞分化因子受容体RANKのプロモータ領域メチル化による発現制御2018

    • Author(s)
      北澤 理子, 村田 夕紀, 原口 竜摩, 上田 康雄, 福島 万奈, 北澤 荘平
    • Organizer
      日本骨代謝学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 成長板を発生起点とするヘッジホッグシグナル受容細胞の骨格形成への関与2018

    • Author(s)
      原口 竜摩, 北澤 理子, 今井 祐記, 北澤 荘平
    • Organizer
      日本骨代謝学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 長管骨伸長時におけるヘッジホッグシグナル受容細胞の組織系譜解析2017

    • Author(s)
      原口 竜摩, 北澤 理子, 今井 祐記, 北澤 荘平
    • Organizer
      日本骨代謝学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの機能解析2017

    • Author(s)
      北澤理子、北澤荘平ほか
    • Organizer
      日本骨代謝学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 下部消化管糖尿病合併症におけるヘッジホッグシグナル経路の関与2017

    • Author(s)
      倉田菜央、北澤荘平ほか
    • Organizer
      日本組織細胞化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 糖尿病による精子形成能低下とヘッジホッグシグナル経路の関連性について2017

    • Author(s)
      大野 輝之, 原口 竜摩, 齋藤 洋太, 下山 貴幸, 北澤 理子, 北澤 荘平
    • Organizer
      日本病理学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2017

    • Author(s)
      北澤理子、北澤荘平ほか
    • Organizer
      日本病理学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 破骨細胞におけるヘッジホッグシグナル伝達系の機能解析2016

    • Author(s)
      原口 竜摩, 北澤 理子, 今井 祐記, 北澤 荘平
    • Organizer
      日本組織細胞化学会総会
    • Place of Presentation
      東京都杏林大学
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2016

    • Author(s)
      北澤 理子,原口 竜摩, 水野 洋輔, 小林 泰浩, 北澤 荘平
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Padlock probeを用いたH-RCA法による組織切片上でのp16遺伝子メチル化シグナル検出2016

    • Author(s)
      二宮 鴻介, 北澤 荘平, 原口 竜摩, 北澤 理子
    • Organizer
      日本病理学会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 髄膜腫における砂粒体形成とカルシウム感知受容体CaSR発現との関連について2016

    • Author(s)
      石村 菜穂, 北澤 理子, 水野 洋輔, 原口 竜摩, 北澤 荘平
    • Organizer
      日本病理学会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] Padlock probeを用いたH-RCA法による病理組織切片上でのDNA1塩基突然変異の検出法の開発2016

    • Author(s)
      沖田 将慶, 北澤 理子, 原口 竜摩, 北澤 荘平
    • Organizer
      日本病理学会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi