| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
We succeeded to construct recombinant proteins of the whole length of OipA and CagA, the important virulence factors of Helicobacter pylori. Using mass spectrometry analyses, our results first suggested that OipA forms multimeric complex within the membrane region of H. pylori, or forms complex with other factors such as CagA. Furthermore, we established the most reliable system in Japan to measure anti-CagA antibody in serum samples. In addition, we succeeded to construct an ex-vivo model of human stomach, gastric epithelial organoid monolayers (gastric monolayers). Using this novel model, we expect to promote our research field on searching the interaction between H. pylori and gastric mucosa.
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