| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
It is widely believed that cell-to-cell viral transfer is a major mode of HIV-1 infection in lymphoid tissues and much more efficient for spreading viral particles than cell-free infection. In our present study, we created a HIV-1 molecular clone carrying 24 repeats of the MS2-binding sites in order to monitor the intracellular dynamics of Gag and vRNA in cells. Using this system, we show that the tumor suppressor adenomatous polyposis coli protein (APC) directs the localization and assembly of HIV-1 Gag polyprotein at distinct membrane components to enable the efficient production and spread of infectious viral particles. Furthermore, we newly identified anti-viral host factors that selectively suppress the cell-to-cell transmission of HIV-1.
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