Validation of supercritical fluid chromatography-mass spectrometry as novel cancer profiling diagnosis system
| Project/Area Number |
16H05227
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | メタボローム解析 / 質量分析 / 超臨界流体 / がん / バイオマーカー / 医療福祉 / 生体分子 / 医療・福祉 / メタボロミクス / 質量分析計 / 超臨界流体抽出 / 超臨界流体クロマトグラフィー |
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| Outline of Final Research Achievements |
We have constructed a new analytical system that connects supercritical fluid extraction (SFE), supercritical fluid chromatography (SFC), and tandem quadrupole mass spectrometer (MS) to analyze in vivo metabolites in human specimens. It was examined whether the analysis could be applied as a new cancer diagnostic method. The constructed SFE-SFC-MS device was used to validate the analysis method for human samples, and a method for comprehensive analysis of metabolites was established. In addition, we analyzed the metabolites in blood of cancer patients and healthy subjects using a liquid chromatograph mass spectrometer, determined cancer metabolite biomarker candidates, and applied them to this system. As a result, it was suggested that the constructed system has the potential as a new cancer diagnostic device.
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| Academic Significance and Societal Importance of the Research Achievements |
低分子代謝物を網羅的に解析するメタボロミクスは、生体分子を高感度で検出できる質量分析計が中核となっている。しかし、質量分析計の取り扱いは煩雑で、専門家でなければ扱えない複雑なものであり、医療応用が進んでいるとは言いがたく、より簡便、かつ、汎用性のある全行程を自動化したシステムの構築が不可欠である。本研究では、オンラインSFE-SFC-MS装置を用いて、DBSを用いて血中代謝物バイオマーカー候補を分析できる系を構築し、さらには、実際の患者検体の分析を実施し、このシステムの有用性について評価することを目的としている。これらは、医療への分析機器の実用化という観点からすれば、非常に有益であると考える。
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Investigations in the possibility of early detection of colorectal cancer by gas chromatography/triple-quadrupole mass spectrometry2017
Author(s)
Nishiumi S., Kobayashi T., Kawana S., Unno Y., Sakai T., Okamoto K., Yamada Y., Sudo K., Yamaji T., Saito Y., Kanemitsu Y., Tsuda Okita N., Saito H., Tsugane S., Azuma T., Ojima N., Yoshida M.
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Journal Title
Oncotarget
Volume: 8
Issue: 10
Pages: 17115-17126
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 4.Association between plasma levels of branched-chain amino acid and risk of pancreatic cancer in a large cohort2019
Author(s)
Ryoko Katagiri, Atsushi Goto, Shin Nishiumi, Takashi Kobayashi, Akihisa Hidaka, Sanjeev Budhathoki, Taiki Yamaji, Norie Sawada, Taichi Shimazu, Manami Inoue, Motoki Iwasaki, Masaru Yoshida, Shoichiro Tsugane.
Organizer
第 78 回日本癌学会学術総会
Related Report
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