| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
We have constructed a new analytical system that connects supercritical fluid extraction (SFE), supercritical fluid chromatography (SFC), and tandem quadrupole mass spectrometer (MS) to analyze in vivo metabolites in human specimens. It was examined whether the analysis could be applied as a new cancer diagnostic method. The constructed SFE-SFC-MS device was used to validate the analysis method for human samples, and a method for comprehensive analysis of metabolites was established. In addition, we analyzed the metabolites in blood of cancer patients and healthy subjects using a liquid chromatograph mass spectrometer, determined cancer metabolite biomarker candidates, and applied them to this system. As a result, it was suggested that the constructed system has the potential as a new cancer diagnostic device.
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