Development of novel diagnostic methods of carcinoma focused on epithelial-to-mesenchymal transition (EMT)

• Project/Area Number: 16H05230
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Yokohama City University
• Principal Investigator: Kimura Yayoi 横浜市立大学, 先端医科学研究センター, 准教授 (80391936)
• Co-Investigator(Kenkyū-buntansha): 大島 貴 横浜市立大学, 医学研究科, 客員教授 (10448665)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: プロテオミクス / 上皮間葉転換(EMT) / バイオマーカー探索 / 質量分析 / 上皮間葉転換 / 診断法 / 癌 / プロテオーム

Outline of Final Research Achievements

The epithelial-to-mesenchymal transition (EMT) is a unique process for the phenotypic changes of tumor cells characterized by a transition from epithelial cells to migrant mesenchymal cells. Therefore, EMT-related proteins are expected as potential candidates of cancer biomarkers involved in tumor invasion and metastasis. This study aimed to a develop novel diagnostic method of carcinoma focused on EMT. To this purpose, the functional roles of the EMT-related protein, which is specifically expressed in the lung adenocarcinoma cells with TGF-beta stimulation, were investigated. In order to discover novel serum biomarkers, we also improved a method of serum proteome analysis by DIA in mass spectrometry. Through the DIA analysis, we explored potential serum biomarkers for tumor type annotation of cancer.

Academic Significance and Societal Importance of the Research Achievements

本研究は、悪性度の高い癌を区別するための新規診断法の構築を目指している。これは、癌医療における治療法選択のための診断基準としても利用でき、将来的には、各人に必要な治療のみを行う個別化医療の実現を可能とし、患者らの身体的・精神的負担の軽減、さらには有効な治療法の選択による治療成績の向上ならびに医療費の削減につながると期待される。また、生体内におけるEMT細胞の存在と癌の悪性度との関係を証明する研究になる可能性があり、今後の創薬を含む先進的な治療法や予防法の開発に寄与することも大いに期待され、癌医療の発展においても非常に意義のある研究になると考えられる。

Research Products

• [Journal Article] Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers (2019)
  • Author(s): E. Kinoshita-Kikuta, E. Kinoshita, S. Ueda, Y. Ino, Y. Kimura, H. Hirano, and T. Koike
  • Journal Title: BBA - Proteins and Proteomics Volume: 1867 Issue: 1 Pages: 62-70
  • DOI: 10.1016/j.bbapap.2018.05.004
  • Peer Reviewed / Open Access
• [Journal Article] Two distinct mechanisms target the autophagy-related E3 complex to the pre-autophagosomal structure (2019)
  • Author(s): Harada Kumi、Kotani Tetsuya、Kirisako Hiromi、Sakoh-Nakatogawa Machiko、Oikawa Yu、Kimura Yayoi、Hirano Hisashi、Yamamoto Hayashi、Ohsumi Yoshinori、Nakatogawa Hitoshi
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.43088
  • Peer Reviewed / Open Access
• [Journal Article] PIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation (2019)
  • Author(s): Miyakawa Kei、Matsunaga Satoko、Yokoyama Masaru、Nomaguchi Masako、Kimura Yayoi、Nishi Mayuko、Kimura Hirokazu、Sato Hironori、Hirano Hisashi、Tamura Tomohiko、Akari Hirofumi、Miura Tomoyuki、Adachi Akio、Sawasaki Tatsuya、Yamamoto Naoki、Ryo Akihide
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1844-1844
  • DOI: 10.1038/s41467-019-09867-7
  • NAID: 120006949469
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Proteome and behavioral alterations in phosphorylation-deficient mutant Collapsin Response Mediator Protein2 knock-in mice. (2018)
  • Author(s): Nakamura H, Jitsuki-Takahashi A, Makihara H, Asano T, Kimura Y, Nakabayashi J, Yamashita N, Kawamoto Y, Nakamura F, Ohshima T, Hirano H, Tanaka F, Goshima Y
  • Journal Title: Neurochemistry international Volume: 119 Pages: 207-217
  • DOI: 10.1016/j.neuint.2018.04.009
  • Peer Reviewed
• [Journal Article] Protein fractionation for proteomics using the SAINOME-plate (2018)
  • Author(s): Ino Yoko、Kagawa Hiroyuki、Akiyama Tomoko、Nakai Yusuke、Ito Sakura、Shimoda Masahiro、Kawamura Makiko、Hirano Hisashi、Kimura Yayoi
  • Journal Title: Journal of Electrophoresis Volume: 62 Issue: 1 Pages: 11-15
  • DOI: 10.2198/jelectroph.62.11
  • NAID: 130007410019
  • ISSN: 1349-9394, 1349-9408
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of secreted protein, acidic and cysteine-rich gene expression in patients with stage II/III gastric cancer following curative resection and adjuvant chemotherapy with S-1 (2018)
  • Author(s): Suzuki Yoshihiro、Oshima Takashi、Yoshihara Kazue、Sakamaki Kentaro、Aoyama Toru、Cho Haruhiko、Shiozawa Manabu、Yoshikawa Takaki、Rino Yasushi、Imada Toshio、Masuda Munetaka
  • Journal Title: Oncology Letters Volume: 15 Pages: 7335-7343
  • DOI: 10.3892/ol.2018.8248
  • Peer Reviewed / Open Access
• [Journal Article] EZH2 Overexpression as a Useful Prognostic Marker for Aggressive Behaviour in Thyroid Cancer (2018)
  • Author(s): Masudo K, Suganuma N, Nakayama H, Oshima T, Rino Y, Iwasaki H, Matsuzu K, Sugino K, Ito K, Kondo T, Nakamura Y, Yoshihara M, Masuda M, Miyagi Y.
  • Journal Title: In Vivo Volume: 32 Issue: 1 Pages: 25-31
  • DOI: 10.21873/invivo.11200
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of the Transmembrane Protein IQGAP3 Is Associated with Poor Survival of Patients with Gastric Cancer. (2017)
  • Author(s): Oue N, Yamamoto Y, Oshima T, Asai R, Ishikawa A, Uraoka N, Sakamoto N, Sentani K, Yasui W.
  • Journal Title: Pathobiology Volume: - Issue: 3 Pages: 192-200
  • DOI: 10.1159/000481890
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mapping the Interactome of a Major Mammalian Endoplasmic Reticulum Heat Shock Protein 90. (2017)
  • Author(s): Hong F, Mohammad Rachidi S, Lundgren D, Han D, Huang X, Zhao H, Kimura Y, Hirano H, Ohara O, Udono H, Meng S, Liu B, Li Z.
  • Journal Title: PLoS One Volume: 12(1) Issue: 1 Pages: e0169260-e0169260
  • DOI: 10.1371/journal.pone.0169260
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of candidate diagnostic serum biomarkers for Kawasaki disease using proteomic analysis (2017)
  • Author(s): Kimura Yayoi、Yanagimachi Masakatsu、Ino Yoko、Aketagawa Mao、Matsuo Michie、Okayama Akiko、Shimizu Hiroyuki、Oba Kunihiro、Morioka Ichiro、Imagawa Tomoyuki、Kaneko Tetsuji、Yokota Shumpei、Hirano Hisashi、Mori Masaaki
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 43732-43732
  • DOI: 10.1038/srep43732
  • NAID: 120006373781
  • Peer Reviewed / Open Access
• [Journal Article] A Phos-tag-based micropipette-tip method for rapid and selective enrichment of phosphopeptides (2017)
  • Author(s): E. Tianfei Yuan, Y. Ino, M. Kawaguchi, Y. Kimura, H. Hirano, E. Kinoshita-Kikuta, E. Kinoshita, and Tohru Koike
  • Journal Title: Electrophoresis Volume: 38 Issue: 19 Pages: 2447-2455
  • DOI: 10.1002/elps.201700175
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The effects of heat stress on morphological properties and intracellular signaling of denervated and intact soleus muscles in rats (2017)
  • Author(s): Ohira Takashi、Higashibata Akira、Seki Masaya、Kurata Yoichi、Kimura Yayoi、Hirano Hisashi、Kusakari Yoichiro、Minamisawa Susumu、Kudo Takashi、Takahashi Satoru、Ohira Yoshinobu、Furukawa Satoshi
  • Journal Title: Physiol Rep Volume: 5 Issue: 15 Pages: e13350-e13350
  • DOI: 10.14814/phy2.13350
  • NAID: 120007134851
  • Peer Reviewed / Open Access
• [Journal Article] Matrix metalloproteinase-7 induces homotypic tumor cell aggregation via proteolytic cleavage of the membrane-bound Kunitz-type inhibitor HAI-1. (2017)
  • Author(s): Ishikawa T, Kimura Y, Hirano H, Higashi S
  • Journal Title: The Journal of Biological Chemistry Volume: 292 Issue: 50 Pages: 20769-20784
  • DOI: 10.1074/jbc.m117.796789
  • Peer Reviewed / Open Access
• [Journal Article] Phos-tag World: The path to the future of electrophoresis, pioneered by the Phos-tag (2017)
  • Author(s): Kimura Yayoi、Ino Yoko、Hirano Hisashi
  • Journal Title: Electrophoresis Letters Volume: 61 Issue: 2 Pages: 45-48
  • DOI: 10.2198/electroph.61.45
  • NAID: 130006223957
  • ISSN: 2189-2628, 2189-2636
  • Peer Reviewed
• [Journal Article] ModProt: A database for integrating laboratory and literature data concerning protein post-translational modifications (2017)
  • Author(s): Kimura Yayoi、Toda Tosifusa、Hirano Hisashi
  • Journal Title: Electrophoresis Letters Volume: 61 Issue: 1 Pages: 5-8
  • DOI: 10.2198/electroph.61.5
  • NAID: 130005863667
  • ISSN: 2189-2628, 2189-2636
  • Peer Reviewed
• [Journal Article] Expression of enhancer of zeste homolog 2 correlates with survival outcome in patients with metastatic breast cancer: exploratory study using primary and paired metastatic lesions (2017)
  • Author(s): Inari Hitoshi、Suganuma Nobuyasu、Kawachi Kae、Yoshida Tatsuya、Yamanaka Takashi、Nakamura Yoshiyasu、Yoshihara Mitsuyo、Nakayama Hirotaka、Yamanaka Ayumi、Masudo Katsuhiko、Oshima Takashi、Yokose Tomoyuki、Rino Yasushi、Shimizu Satoru、Miyagi Yohei、Masuda Munetaka
  • Journal Title: BMC Cancer Volume: 17 Issue: 1 Pages: 160-160
  • DOI: 10.1186/s12885-017-3154-3
  • Peer Reviewed / Open Access
• [Journal Article] Clinicopathological and prognostic significance of Ki-67 immunohistochemical expression of distant metastatic lesions in patients with metastatic breast cancer (2017)
  • Author(s): Inari Hitoshi、Suganuma Nobuyasu、Kawachi Kae、Yoshida Tatsuya、Yamanaka Takashi、Nakamura Yoshiyasu、Yoshihara Mitsuyo、Nakayama Hirotaka、Masudo Katsuhiko、Oshima Takashi、Yokose Tomoyuki、Rino Yasushi、Shimizu Satoru、Miyagi Yohei、Masuda Munetaka
  • Journal Title: Breast Cancer Volume: 24 Issue: 6 Pages: 748-755
  • DOI: 10.1007/s12282-017-0774-z
  • Peer Reviewed
• [Journal Article] Clinical implications of ribonucleotide reductase subunit M1 in patients with pancreatic cancer who undergo curative resection followed by adjuvant chemotherapy with gemcitabine (2017)
  • Author(s): Aoyama Toru、Miyagi Yohei、Murakawa Masaaki、Yamaoku Koichiro、Atsumi Yosuke、Shiozawa Manabu、Ueno Makoto、Morimoto Manabu、Oshima Takashi、Yukawa Norio、Yoshikawa Takaki、Rino Yasushi、Masuda Munetaka、Morinaga Soichiro
  • Journal Title: Oncology Letters Volume: 13 Issue: 5 Pages: 3423-3430
  • DOI: 10.3892/ol.2017.5935
  • Peer Reviewed / Open Access
• [Journal Article] Clinicopathological significance and impact on outcomes of the gene expression levels of IGF-1, IGF-2 and IGF-1R, IGFBP-3 in patients with colorectal cancer: Overexpression of the IGFBP-3 gene is an effective predictor of outcomes in patients with colorectal cancer (2017)
  • Author(s): Yamamoto Naoto、Oshima Takashi、Yoshihara Kazue、Aoyama Toru、Hayashi Tsutomu、Yamada Takanobu、Sato Tsutomu、Shiozawa Manabu、Yoshikawa Takaki、Morinaga Soichiro、Rino Yasushi、Kunisaki Chikara、Tanaka Katsuaki、Akaike Makoto、Imada Toshio、Masuda Munetaka
  • Journal Title: Oncology Letters Volume: 13 Issue: 5 Pages: 3958-3966
  • DOI: 10.3892/ol.2017.5936
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Significance of INHBA Gene Expression in Patients with Gastric Cancer who Receive Curative Resection Followed by Adjuvant S-1 Chemotherapy (2017)
  • Author(s): Katayama Y, Oshima T, Sakamaki K, Aoyama T, Sato T, Masudo K, Shiozawa M, Yoshikawa T, Rino Y, Imada T, Masuda M.
  • Journal Title: In Vivo Volume: 31 Issue: 4 Pages: 565-571
  • DOI: 10.21873/invivo.11095
  • Peer Reviewed / Open Access
• [Journal Article] Clinical implications of dihydropyrimidine dehydrogenase expression in patients with pancreatic cancer who undergo curative resection with S-1 adjuvant chemotherapy (2017)
  • Author(s): Murakawa Masaaki、Aoyama Toru、Miyagi Yohei、Atsumi Yosuke、Kazama Keisuke、Yamaoku Koichiro、Kanazawa Amane、Shiozawa Manabu、Kobayashi Satoshi、Ueno Makoto、Morimoto Manabu、Yamamoto Naoto、Oshima Takashi、Yoshikawa Takaki、Rino Yasushi、Masuda Munetaka、Morinaga Soichiro
  • Journal Title: Oncology Letters Volume: 14 Issue: 2 Pages: 1505-1511
  • DOI: 10.3892/ol.2017.6295
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of PCDHB9 promotes peritoneal metastasis and correlates with poor prognosis in patients with gastric cancer. (2017)
  • Author(s): Mukai S, Oue N, Oshima T, Imai T, Sekino Y, Honma R, Sakamoto N, Sentani K, Kuniyasu H, Egi H, Tanabe K, Yoshida K, Ohdan H, Yasui W.
  • Journal Title: J Pathol Volume: 243 Issue: 1 Pages: 100-110
  • DOI: 10.1002/path.4931
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Significance of Tensin 4 Gene Expression in Patients with Gastric Cancer (2017)
  • Author(s): Sawazaki S, Oshima T, Sakamaki K, Aoyama T, Sato T, Shiozawa M, Yoshikawa T, RinoY, Imada T, Masuda M.
  • Journal Title: In Vivo Volume: 31 Issue: 6 Pages: 1065-1071
  • DOI: 10.21873/invivo.11171
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of platelet-derived growth factor receptor-β gene expression in stage II/III gastric cancer with S-1 adjuvant chemotherapy. (2017)
  • Author(s): Higuchi A, Oshima T, Yoshihara K, Sakamaki K, Aoyama T, Suganuma N, Yamamoto N, Sato T, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Imada T, Masuda M.
  • Journal Title: Oncology letters Volume: 13 Issue: 2 Pages: 905-911
  • DOI: 10.3892/ol.2016.5494
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of KIF11 in gastric cancer with intestinal mucin phenotype (2016)
  • Author(s): Imai T, Oue N, Nishioka M, Mukai S, Oshima T, Sakamoto N, Sentani K, Matsusaki K, Yoshida K, Yasui W
  • Journal Title: Pathobiology Volume: 84 Issue: 1 Pages: 16-24
  • DOI: 10.1159/000447303
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overexpression of transmembrane protein BST2, participating in cell growth, is associated with poor survival in patients with esophageal, gastric and colorectal cancer (2016)
  • Author(s): Mukai S, Oue N, Oshima T, Tatsumoto Y, Sakamoto N, Sentani N, Hinoi T, Ohdan H, Yasui W
  • Journal Title: Annals of Surgical Oncology Volume: 24 Issue: 2 Pages: 594-602
  • DOI: 10.1245/s10434-016-5100-z
  • Peer Reviewed / Open Access
• [Journal Article] Biological significance of co- and post-translational modifications of the yeast 26S proteasome. (2016)
  • Author(s): Hirano H, Kimura Y, Kimura A
  • Journal Title: J. proteomics Volume: 134 Pages: 37-46
  • DOI: 10.1016/j.jprot.2015.11.016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress (2016)
  • Author(s): Pu Zijing、Ino Yoko、Kimura Yayoi、Tago Asumi、Shimizu Motoki、Natsume Satoshi、Sano Yoshitaka、Fujimoto Ryo、Kaneko Kentaro、Shea Daniel J.、Fukai Eigo、Fuji Shin-Ichi、Hirano Hisashi、Okazaki Keiichi
  • Journal Title: Frontiers in Plant Science Volume: 7 Pages: 31-31
  • DOI: 10.3389/fpls.2016.00031
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma (2016)
  • Author(s): Okayama Akiko、Kimura Yayoi、Miyagi Yohei、Oshima Takashi、Oshita Fumihiro、Ito Hiroyuki、Nakayama Haruhiko、Nagashima Takuya、Rino Yasushi、Masuda Munetaka、Ryo Akihide、Hirano Hisashi
  • Journal Title: Journal of Proteomics Volume: 139 Pages: 60-66
  • DOI: 10.1016/j.jprot.2016.03.005
  • Peer Reviewed
• [Journal Article] Identification of glycosylphosphatidylinositol-anchored proteins and ω-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment. (2016)
  • Author(s): Masuishi Y, Kimura Y, Arakawa N, Hirano H.
  • Journal Title: J Proteomics Volume: 139 Pages: 77-83
  • DOI: 10.1016/j.jprot.2016.03.008
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Data for identification of GPI-anchored peptides and ω-sites in cancer cell lines (2016)
  • Author(s): Yusuke Masuishi, Yayoi Kimura, Noriaki Arakawa, Hisashi Hirano
  • Journal Title: Data in Brief Volume: 7 Pages: 1302-1305
  • DOI: 10.1016/j.dib.2016.04.001
  • Peer Reviewed / Open Access
• [Journal Article] Micro-adhesion rings surrounding TCR microclusters are essential for T cell activation (2016)
  • Author(s): Hashimoto-Tane A, Sakuma M, Ike H, Yokosuka T, Kimura Y, Ohara O, Saito T.
  • Journal Title: J. Exp. Med. Volume: 213 Issue: 8 Pages: 1609-1625
  • DOI: 10.1084/jem.20151088
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes. (2016)
  • Author(s): Yamamoto H, Fujioka Y, Suzuki SW, Noshiro D, Suzuki H, Kondo-Kakuta C, Kimura Y, Hirano H, Ando T, Noda NN, Ohsumi Y
  • Journal Title: Developmental Cell Volume: 38 Issue: 1 Pages: 86-99
  • DOI: 10.1016/j.devcel.2016.06.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lyn Kinase Suppresses the Transcriptional Activity of IRF5 in the TLR-MyD88 Pathway to Restrain the Development of Autoimmunity. (2016)
  • Author(s): Ban T., Sato G.R., Nishiyama A., Akiyama A., Takasuna M., Umehara M., Suzuki S., Ichino M., Matsunaga S., Kimura A., Kimura Y., Yanai H., Miyashita S., Kuromitsu J., Tsukahara K., Yoshimatsu K., Endo I., Yamamoto T., Hirano H., Ryo A., Taniguchi T., Tamura T.
  • Journal Title: Immunity Volume: 45 Issue: 2 Pages: 319-332
  • DOI: 10.1016/j.immuni.2016.07.015
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comprehensive behavioral study and proteomic analyses of CRMP2-deficient mice. (2016)
  • Author(s): Nakamura H, Yamashita N, Kimura A, Kimura Y, Hirano H, Makihara H, Kawamoto Y, Jitsuki-Takahashi A, Yonezaki K, Takase K, Miyazaki T, Nakamura F, Tanaka F, Goshima Y.
  • Journal Title: Genes to Cells Volume: 21 Issue: 10 Pages: 1059-1079
  • DOI: 10.1111/gtc.12403
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of IGF1R gene expression in patients with Stage II/III gastric cancer who receive curative surgery and adjuvant chemotherapy with S-1. (2016)
  • Author(s): Numata K, Oshima T, Sakamaki K, Yoshihara K, Aoyama T, Hayashi T, Yamada T, Sato T, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Akaike M, Imada T, Masuda M.
  • Journal Title: Journal of Cancer Research and Clinical Oncology Volume: 142 Issue: 2 Pages: 415-422
  • DOI: 10.1007/s00432-015-2039-6
  • Peer Reviewed
• [Presentation] 骨格筋タンパク質の比較定量解析手法の確立 (2018)
  • Author(s): 1.井野洋子, 大平宇志, 森山佳谷乃, 中居佑介, 木村鮎子, 熊谷研, 齋藤知行, 森田啓之, 木村弥生, 平野久.
  • Organizer: MSP2018（日本質量分析学会・日本プロテオーム学会2018年合同大会）
• [Presentation] DIA法を利用した後肢懸垂マウス血清の定量プロテオーム解析 (2018)
  • Author(s): 2.中居佑介, 木村鮎子, 森山佳谷乃, 香川裕之, 井野洋子, 大平宇志, 木村弥生, 平野久
  • Organizer: MSP2018（日本質量分析学会・日本プロテオーム学会2018年合同大会）
• [Presentation] 個別化治療を目指したStageII/III胃癌根治切除後の予後層別化マーカー検索. (2018)
  • Author(s): 3.大島 貴, 宮城 洋平, 木村 弥生, 大上 直秀, 坂巻 顕太郎, 橋本 至, 山中 正二, 青山 徹, 塩澤 学, 吉川 貴己, 森永 聡一郎, 利野 靖, 安井 弥, 益田 宗孝.
  • Organizer: 第56回日本癌治療学会学術集会
• [Presentation] プロテオーム解析技術の発展と疾患バイオマーカー探索 (2018)
  • Author(s): 木村弥生
  • Organizer: 第15回北里疾患プロテオーム研究会
  • Invited
• [Presentation] DIA法を用いたハイスループットな血清プロテオーム解析 (2017)
  • Author(s): 1.伴涼太朗,中居佑介,井野洋子,宮本優子,香川裕之,F.Schmidt,平野久,木村弥生
  • Organizer: 日本プロテオーム学会 2017年大会（JHUPO第15回大会）
• [Presentation] pplication of High-throughput Serum Proteomic Strategy Using DIA-MS (2017)
  • Author(s): Ryotaro Ban, Yusuke Nakai, Yoko Ino, Yuko Miyamoto, Hiroyuki Kagawa, Frank Schmidt, Hisashi Hirano, Yayoi Kimura.
  • Organizer: HUPO2017, the 16th Annual World Congress
  • Int'l Joint Research
• [Presentation] MSを用いたDIA法によるハイスループット多検体血清定量解析手法の開発. (2017)
  • Author(s): 中居佑介,伴涼太朗,井野洋子,香川裕之,平野久,木村弥生.
  • Organizer: 2017年度生命科学系学会合同年次大会 (ConBio2017)
• [Presentation] Phos-tagわーるど！ ～Phos-tagが拓く電気泳動の未来～ (2017)
  • Author(s): 木村弥生.
  • Organizer: 第69回 日本電気泳動学会総会
  • Invited
• [Presentation] How can we effectively detect biomarker candidates by mass spectrometry? (2017)
  • Author(s): Hirano H, Kimura Y, Arakawa N, Okayama A, Kimura A, Ino Y, Kagawa H.
  • Organizer: HUPO2017, the 16th Annual World Congress
  • Int'l Joint Research
• [Presentation] サイノメプレートを用いたタンパク質の分画 (2017)
  • Author(s): 井野洋子, 香川裕之, 秋山知子, 中居佑介, 伊藤さくら, 下田昌弘, 河村眞紀子, 平野久, 木村弥生.
  • Organizer: 第68回日本電気泳動学会総会
• [Presentation] Identification of candidate diagnostic serum biomarkers for Kawasaki disease using proteomic analysis. (2017)
  • Author(s): Ino Y, Yanagimachi M, Mori M, Hirano H, Kimura Y.
  • Organizer: HUPO2017, the 16th Annual World Congress
  • Int'l Joint Research
• [Presentation] クロマチン再構成複合体因子BRG1のリン酸化修飾によるクロマチン関連タンパク質の変化と遺伝子発現制御 (2017)
  • Author(s): Kimura A, Nakabayashi J, Tamura T, Kagawa H, Kimura Y, Hirano H
  • Organizer: 2017年度生命科学系学会合同年次大会 (ConBio2017)
• [Presentation] Proteome and transcriptome analyses of phosphorylation site-specific mutants of BRG1, A chromatin remodeling component. (2017)
  • Author(s): Kimura A, Nakabayashi J, Tamura T, Kagawa H, Kimura Y, Hirano, H.
  • Organizer: HUPO2017, the 16th Annual World Congress
  • Int'l Joint Research
• [Presentation] 高感度リン酸化プロテオーム解析 (2016)
  • Author(s): 木村弥生
  • Organizer: 日本プロテオーム学会
  • Invited
• [Presentation] 各臓器におけるGPIアンカー型タンパク質発現プロファイル (2016)
  • Author(s): 増石有佑、木村弥生、荒川憲昭、平野久
  • Organizer: 日本プロテオーム学会
• [Presentation] TGF-β (β型変異増殖因子)を利用した癌転移メカニズムの解明 (2016)
  • Author(s): 岡山明子, 木村弥生, 梁明秀, 平野久
  • Organizer: 日本プロテオーム学会
• [Presentation] 新規バイオマーカー探索に向けた糖ペプチド濃縮法の開発 (2016)
  • Author(s): 中居佑介、石山 歩、岡山明子、平野 久、木村弥生
  • Organizer: 日本プロテオーム学会
• [Presentation] H.Glycosylphosphatidylinositol-anchored Proteins Profile in Organs (2016)
  • Author(s): Masuishi, Y., Kimura, Y., Arakawa, N. and Hirano, H.
  • Organizer: HUPO 2016, the 15th Annual World Congress 2016
  • Int'l Joint Research
• [Presentation] ModProt: A database for integrating laboratory and literature data concerning protein post-translational modifications (2016)
  • Author(s): Kimura, Y., Hirano, H., and Toda, T.
  • Organizer: HUPO 2016, the 15th Annual World Congress 2016
  • Int'l Joint Research
• [Presentation] Proteomic analysis of TGF-β-induced cancer metastasis (2016)
  • Author(s): Okayama, A., Kimura, Y., Ryo, A. and Hirano, H.
  • Organizer: HUPO 2016, the 15th Annual World Congress 2016
  • Int'l Joint Research
• [Presentation] Phosphate-affinity chromatographic micro-tip technology for enrichment of phosphopeptides towards phosphoproteomic study. (2016)
  • Author(s): Kawaguchi, M., Yuan, E. T., Ino, Y., Kimura, Y., Hirano, H., Kinoshita-Kikuta, E., Kinoshita, E., Koike, T.
  • Organizer: HUPO 2016, the 15th Annual World Congress 2016
  • Int'l Joint Research
• [Presentation] Effects of co-/post-translational modifications on protein function. (2016)
  • Author(s): Kimura, Y., Kimura, A., Okayama, A., Ino, Y., Kurata, Y., Kagawa, H., Toda, T., Hirano, H.
  • Organizer: HUPO 2016, the 15th Annual World Congress 2016
  • Int'l Joint Research
• [Presentation] Determining Post-Translational Modifications of Nuclear Proteins. (2016)
  • Author(s): Ban, R., Akiyama, T., Ino, Y., Nakai, Y., Kagawa, H., Toda, T., Kawasaki, N., Hirano, H., Kimura, Y.
  • Organizer: HUPO 2016, the 15th Annual World Congress 2016
  • Int'l Joint Research
• [Presentation] 核内構成タンパク質の翻訳後修飾解析 (2016)
  • Author(s): 伴涼太郎, 秋山知子, 井野洋子, 香川裕之, 川崎ナナ, 平野 久, 木村弥生
  • Organizer: 日本プロテオーム学会
• [Presentation] 培養がん細胞においてMMP-7がセリンプロテアーゼ様活性を促進する機構の解析 (2016)
  • Author(s): 石川智弘, 木村弥生, 平野 久, 東 昌市
  • Organizer: 日本生化学会大会
• [Book] 材料表面の親水・親油の評価と制御設計 (2016)
  • Author(s): 石井淑夫監修
  • Total Pages: 600
  • Publisher: テクノシステム