| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Outline of Final Research Achievements |
We have extracted DNAs from paraffin embedded pathological specimens from patients with cholestatic liver diseases such as primary biliary cholangitis. We have used laser micro-dissection methods to extract DNAs, and constructed the libraries from deep sequencing. Similarly, we extracted DNAs from serum samples with identical patients, and compared their libraries along with their clinical backgrounds. After adequate adjustments, we have found novel external 16rRNAs from both liver and serum samples. We have identified these pathogens at phyrum levels. To further identify these exogenous genes, adequate primer designing seems to be feasible.
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