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Precision Medicine for Colon Cancer by High-throughput Analysis by Quantitative Mass Spectrometry

Research Project

Project/Area Number 16H05289
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Naito Yuji  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00305575)

Co-Investigator(Kenkyū-buntansha) 内山 和彦  京都府立医科大学, 医学(系)研究科(研究院), 助教 (50298428)
吉田 直久  京都府立医科大学, 医学(系)研究科(研究院), 講師 (50340089)
高木 智久  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70405257)
東村 泰希  石川県立大学, 生物資源環境学部, 准教授 (70628924)
半田 修  京都府立医科大学, 医学(系)研究科(研究院), 特任講師 (90381970)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords大腸癌 / バイオマーカー / 早期発見 / ペプチド / 質量分析 / ペプチドマーカー / 質量分析計 / 先制医療 / ペプチド分析 / リキッドバイオプシー
Outline of Final Research Achievements

Colorectal cancer (CRC) is one of the most predominant types of cancer, and it is the fourth most common cause of cancer-related death and it is important to diagnose CRC in early stage to decrease the mortality by CRC. A total of 56 patients with CRC (n = 14 each of stages I-IV), 60 healthy controls, and 60 patients with colonic adenoma were included in this study. The five peptides were extracted and analyzed by selected reaction monitoring using ProtoKey, Colorectal Cancer Risk Test Kit (Protosera, Inc., Amagasaki, Japan). The results clearly showed that the four CRC groups, stages I-IV, could be sufficiently discriminated from the control group and colonic polyp group. This five-peptide set could identify CRC at each stage compared to the control population in this validation cohort, including those with early-stage disease. The AUC values for each stage of CRC compared to the control population were 0.779, 0.946, 0.852, and 0.973 for stages I, II, III, and IV, respectively.

Academic Significance and Societal Importance of the Research Achievements

世界的にみてもわが国の大腸癌死亡率は極めて高く、大腸癌対策は緊喫の臨床的課題であり、大腸癌の早期診断・治療・予防に向けた取り組みは極めて重要な国家戦略となりつつある。侵襲度の高い生体検査なしに血液で大腸癌を診断するリキッドバイオプシーの開発がニーズも高く重要な研究である。本研究の結果、大腸癌のステージ毎のペプチドマーカーによる診断能は、感度、特異度とも極めて高感度、高特異度であり、大腸がん検診における新たな検診の方向性を見いだす社会的意義は極めて大きい。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] Selected reaction monitoring for colorectal cancer diagnosis using a set of five serum peptides identified by BLOTCHIP((R))-MS analysis2018

    • Author(s)
      Uchiyama, K. Naito, Y. Yagi, N. Mizushima, K. Higashimura, Y. Hirai, Y. Dohi, O. Okayama, T. Yoshida, N. Katada, K. Kamada, K. Handa, O. Ishikawa, T. Takagi, T. Konishi, H. Nonaka, D. Asada, K. Lee, L. J. Tanaka, K. Kuriu, Y. Nakanishi, M. Otsuji, E. Itoh, Y.
    • Journal Title

      J Gastroenterol

      Volume: 印刷中 Issue: 11 Pages: 1179-1185

    • DOI

      10.1007/s00535-018-1448-0

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The protective effect of orally administered redox nanoparticle on intestinal ischemia-reperfusion injury in mice.2018

    • Author(s)
      Ueda T, Takagi T, Katada K, Iida T, Mizushima K, Dohi O, Okayama T, Yoshida N, Kamada K, Uchiyama K, Handa O, Ishikawa T, Konishi H, Naito Y, Nagasaki Y, Itoh Y.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 495 Issue: 2 Pages: 2044-2049

    • DOI

      10.1016/j.bbrc.2017.11.204

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Secreted protein acidic and rich in cysteine functions in colitis via IL17A regulation in mucosal CD4(+) T cells.2018

    • Author(s)
      Tanaka M, Takagi T, Naito Y, Uchiyama K, Hotta Y, Toyokawa Y, Ushiroda C, Hirai Y, Aoi W, Higashimura Y, Mizushima K, Okayama T, Katada K, Kamada K, Ishikawa T, Handa O, Itoh Y.
    • Journal Title

      J Gastroenterol Hepatol.

      Volume: 33 Issue: 3 Pages: 671-680

    • DOI

      10.1111/jgh.13842

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serum metabolomics analysis for early detection of colorectal cancer2017

    • Author(s)
      Kazuhiko Uchiyama, Nobuaki Yagi, Katsura Mizushima, Yasuki Higashimura, Yasuko Hirai, Tetsuya Okayama, Naohisa Yoshida, Kazuhiro Katada, Kazuhiro Kamada, Osamu Handa, Takeshi Ishikawa, Tomohisa Takagi, Hideyuki Konishi, Yoshiaki Kuriu, Masayoshi Nakanishi, Eigo Otsuji, Yoshito Itoh, Yuji Naito
    • Journal Title

      Journal of Gastroenterology

      Volume: 52(6) Issue: 6 Pages: 677-694

    • DOI

      10.1007/s00535-016-1261-6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Risk factors for developing colorectal cancer in Japanese patients with ulcerative colitis: a retrospective observational study-CAPITAL (Cohort and Practice for IBD total management in Kyoto-Shiga Links) study I2016

    • Author(s)
      Yoshino, T. Nakase, H. Takagi, T. Bamba, S. Okuyama, Y. Kawamura, T. Oki, T. Obata, H. Kawanami, C. Katsushima, S. Kusaka, T. Tsujikawa, T. Naito, Y. Andoh, A. Kogawa, T.
    • Journal Title

      BMJ Open Gastroenterol

      Volume: 3 Issue: 1 Pages: e000122-e000122

    • DOI

      10.1136/bmjgast-2016-000122

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High incidence of metachronous advanced adenoma and cancer after endoscopic resection of colon polyps >/=20 mm in size2016

    • Author(s)
      Yoshida, N. Naito, Y. Siah, K. T. Murakami, T. Ogiso, K. Hirose, R. Inada, Y. Inoue, K. Konishi, H. Kugai, M. Morimoto, Y. Hasegawa, D. Kanemasa, K. Wakabayashi, N. Yagi, N. Yanagisawa, A. Itoh, Y.
    • Journal Title

      Dig Endosc

      Volume: 28 Issue: 2 Pages: 194-202

    • DOI

      10.1111/den.12551

    • NAID

      130005464989

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 大腸癌リキッドバイオプシーとしてのメタボローム解析2016

    • Author(s)
      内山和彦,八木信明,内藤裕二.
    • Journal Title

      G.I. Research

      Volume: 24 Pages: 36-41

    • Related Report
      2016 Annual Research Report
  • [Presentation] 大腸癌診断における血清ペプチドバイオマーカーの有用性に関する検討2019

    • Author(s)
      内山和彦、八木信明、内藤裕二
    • Organizer
      第105回に本消化器病学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸癌を撲滅するために内視鏡医に必要なこと~大腸癌を診る,治す,防ぐ~2017

    • Author(s)
      内藤裕二
    • Organizer
      第60回日本消化器内視鏡学会東海支部例会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 腸内マイクロバイオームを考える:粘膜関連細菌は重要か?2016

    • Author(s)
      内藤裕二
    • Organizer
      第53回日本消化器免疫学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] 消化管(おなか)は泣いています~腸内フローラが体を変える、脳を活かす~2016

    • Author(s)
      内藤裕二
    • Total Pages
      253
    • Publisher
      ダイヤモンド社
    • Related Report
      2016 Annual Research Report
  • [Book] 人生を変える賢い腸のつくり方~ココロまで整える腸内フローラ活性術~2016

    • Author(s)
      内藤裕二
    • Total Pages
      253
    • Publisher
      ダイヤモンド社
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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