| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
We challenged how we could use the diseased brain to extract information that might be specific and important to the disease. We performed indirect transcriptome analysis by purifying neuronal nuclei and extracting epigenome information from them as a methodology that enables resistance to postmortem changes and neural cell specific information extraction. By using this method, we were able to approach a new pathological condition of Alzheimer's disease. In other words, the function of DNA repair protein BRCA1 in nerve cells was reduced, which resulted in the reduction of nerve cell function. By applying this method, we performed neural cell-specific analysis of histone modification and found that expression changes of genes related to Alzheimer's disease are controlled by epigenetic.
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