• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Precise identification of epitope of immune complex antigen and development of inhibitory agent on their formation specific for rheumatoid arthritis

Research Project

Project/Area Number 16H05344
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Collagenous pathology/Allergology
Research InstitutionNagasaki University

Principal Investigator

OHYAMA Kaname  長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (50437860)

Co-Investigator(Kenkyū-buntansha) 稲嶺 達夫  長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (00549628)
一瀬 邦弘  長崎大学, 医歯薬学総合研究科(医学系), 講師 (60437895)
岸川 直哉  長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (90336181)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2016: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords免疫複合体 / 関節リウマチ / リウマチ学
Outline of Final Research Achievements

To selectively detect circulating immune complex-antigens with enhanced sensitivity, in this study we compared two methods (low-pH elution and tryptic digestion; papain-digestion, elution, and tryptic digestion). Comparison showed that papain-digestion method provided the most selective and sensitive detection of CIC-antigens. Then, we investigated the condition that can selectively elute peptides not including epitope. As a result, the digestion procedure successively using trypsin and Lys-C was appropriate for the selective elution. Now, the study that recoveries TSP-1 immune complexe from the patients with rheumatoid arthritis and identify their epitope is on-going.

Academic Significance and Societal Importance of the Research Achievements

本研究は関節リウマチでおきる異常な免疫反応の根本原因と考えられるタンパク質の異常部位(エピトープ)を全く新しい方法で決定することを目指している。具体的には、患者体内でできる免疫複合体(異常なタンパク質と抗体が結合したもの)を直接調べて、エピトープを特定する。このエピトープを特定できれば、病気の根治だけでなく、従来の免疫抑制療法で危惧される感染症のリスクを回避する治療法の開発につながる可能性がある。
今回の成果で、免疫複合体に含まれる異常なタンパク質のエピトープを特定するための解析条件を決めることができた。今後は、実際の患者の体内にある免疫複合体を使って、病気の原因となるエピトープの特定を進める。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] Saint Louis University/School of Medicine(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Selective, sensitive and comprehensive detection of immune complex antigens by immune complexome analysis with papain-digestion and elution2019

    • Author(s)
      Aibara N, Kamohara C, Chauhan AK, Kishikawa N, Miyata Y, Nakashima M, Kuroda N, Ohyama K
    • Journal Title

      Journal of Immunological Methods

      Volume: 461 Pages: 85-90

    • DOI

      10.1016/j.jim.2018.06.021

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Proteomic approach to profiling immune complex antigens in cerebrospinal fluid samples from patients with central nervous system autoimmune diseases.2018

    • Author(s)
      Aibara N, Ichinose K, Baba M, Nakajima H, Satoh K, Atarashi R, Kishikawa N, Nishida N, Kawakami A, Kuroda N, Ohyama K
    • Journal Title

      Clin Chim Acta

      Volume: 484 Pages: 26-31

    • DOI

      10.1016/j.cca.2018.05.026

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immune complexome analysis of antigens in circulating immune complexes from patients with acute cellular rejection after living donor liver transplantation.2018

    • Author(s)
      Aibara N, Ohyama K, Hidaka M, Kishikawa N, Miyata Y, Takatsuki M, Eguchi S, Kuroda N.
    • Journal Title

      Transplant Immunology

      Volume: 3274 Pages: 30143-0

    • DOI

      10.1016/j.trim.2018.02.011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel anti-suprabasin antibodies may contribute to the pathogenesis of neuropsychiatric systemic lupus erythematosus.2018

    • Author(s)
      Ichinose K, Ohyama K, Furukawa K, Higuchi O, Mukaino A, Satoh K, Nakane S, Shimizu T, Umeda M, Fukui S, Nishino A, Nakajima H, Koga T, Kawashiri SY, Iwamoto N, Tamai M, Nakamura H, Origuchi T, Yoshida M, Kuroda N, Kawakami A.
    • Journal Title

      Clin Immunol.

      Volume: 印刷中 Pages: 123-130

    • DOI

      10.1016/j.clim.2017.11.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Immune complexome analysis reveals specific and frequent presence of immune complex antigens in lung cancer patients: A pilot study.2017

    • Author(s)
      K. Ohyama, H. Yoshimi, N. Aibara, Y. Nakamura, Y. Miyata, H. Sakai, F. Fujita, Y. Imaizumi, A.K. Chauhan, N. Kishikawa, N. Kuroda
    • Journal Title

      International Journal of Cancer

      Volume: 140 Issue: 2 Pages: 370-380

    • DOI

      10.1002/ijc.30455

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 臨床系教員が薬学の強みを意識して考える医師・薬剤師との共同研究2019

    • Author(s)
      大山 要
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 強みを生かせばできる!基礎-臨床双方向の研究展開2018

    • Author(s)
      大山 要
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] イムノコンプレキソーム解析法における免疫複合体抗原選択的な溶出法の検討2018

    • Author(s)
      相原希美,蒲原千裕,Anil Chauhan,岸川直哉,中嶋幹郎,黒田直敬,大山 要
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 肝臓移植患者の血清中免疫複合体の網羅的解析2016

    • Author(s)
      相原希美、大山 要、日高匡章、岸川直哉、黒田直敬
    • Organizer
      第41回日本医用マススペクトル学会年会
    • Place of Presentation
      愛知県産業労働センター(愛知県・名古屋市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Book] Immunoproteomics: Methods and Protocols, Second Edition (Methods in Molecular Biology)2019

    • Author(s)
      N. Aibara, K. Ohyama
    • Publisher
      Springer
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi