| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
We performed polysomnography, circadian rhythm measurement and blood sampling for methylation typing (8 points every 4 hours) for 48 hours for healthy young adults and healthy elderly people over 60 years old. Methylation typing of the entire genome was performed by the microarray system using the Infinium Methylation EPIC BeadChip kit equipped with the 866,895 markers. In order to identify the methylated region involved in sleep and biological rhythm regulatory functions, the time series change of the obtained methylation amount was analyzed by the cosinor method. As a result, there were 16 regions where circadian rhythmicity was recognized in both young and elderly people. These methylation sites could be essential for the formation of circadian signal irrespective of age.
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