Induction of human iTS-P and iTS-L cells using iPS cell technology
| Project/Area Number |
16H05404
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
NOGUCHI Hirofumi 琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
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| Co-Investigator(Kenkyū-buntansha) |
齊藤 一誠 新潟大学, 医歯学系, 准教授 (90404540)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 再生医療 / iTS細胞 / iPS細胞 / 体性幹細胞 / 再生医学 / iTS-P細胞 / 膵組織 / インスリン分泌細胞 |
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| Outline of Final Research Achievements |
We recently demonstrated the generation of mouse induced tissue-specific stem (iTS) cells through transient overexpression of reprogramming factors combined with tissue-specific selection. Here we induced expandable tissue-specific stem/progenitor (iTS) cells from human pancreatic/hepatic tissue through transient expression of genes encoding the reprogramming factors OCT4, p53 shRNA, SOX2, KLF4, L-MYC, and LIN28.
Transfection of episomal plasmid vectors into human pancreatic tissue efficiently generated iTS cells expressing genetic markers of endoderm and tissue-specific progenitors. The iTS cells differentiated into insulin/albumin-producing cells more efficiently than human iPS cells. iTS cells subcutaneously inoculated into immunodeficient mice did not form teratomas. The generation of human iTS cells may have important implications for the clinical application of stem/progenitor cells.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、組織特異的幹細胞を人工的に作製することが可能であることが、ヒト細胞で証明された。この細胞の利点は１）樹立効率がiPS細胞よりも高い、２）分化誘導効率がES/iPS細胞より高い、３）奇形腫形成がなくES/iPS細胞で懸念された未分化細胞残存による腫瘍形成の心配がない、の3点である。この技術は膵・肝のみならず、さまざまな組織に関して応用可能であると考えられるため、画期的な技術であるといえる。今後、臨床応用化へ向けてiTS細胞の有効性・安全性試験を行っていきたい。
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] A novel preservation solution containing a JNK inhibitory peptide efficiently improves islet yield for porcine islet isolation.2019
Author(s)
Noguchi H, Miyagi-Shiohira C, Nakashima Y, Ebi N, Hamada E, Tamaki Y, Kuwae K, Kitamura S, Kobayashi N, Saitoh I, Watanabe M
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Journal Title
Transplantation.
Volume: 103
Issue: 2
Pages: 344-352
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Role of Egr1 on Pancreatic Endoderm Differentiation2018
Author(s)
Tsugata Takako、Nikoh Naruo、Kin Tatsuya、Miyagi-Shiohira Chika、Nakashima Yoshiki、Saitoh Issei、Noguchi Yasufumi、Ueki Hideo、Watanabe Masami、Kobayashi Naoya、Shapiro Andrew M. James、Noguchi Hirofumi
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Journal Title
Cell Medicine
Volume: 10
Pages: 215517901773317-215517901773317
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers.2017
Author(s)
Murakami T, Saitoh I, Sato M, Inada E, Soda M, Oda M, Domon H, Iwase Y, Sawami T, Matsueda K, Terao Y, Ohshima H, Noguchi H, Hayasaki H
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Journal Title
Archives of Oral Biology
Volume: 81
Pages: 110-120
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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