| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We conducted genome-wide DNA methylation analysis of clinical gastric cancer samples to classify gastric cancer into several molecular subtypes, and we also conducted exon-sequencing analysis to identify genetic mutations specifically observed in each subtype. As for high-methylation gastric cancer subtypes, we clarified molecular mechanisms of gastric cancer through synergy of genetic mutations and aberrant DNA methylation. Moreover, we analyzed the mechanisms to induce aberrant epigenomic aberrations in gastric epithelial cells. We infected Epstein-Barr virus to gastric epithelial cells in vitro, and showed that EBV infection induced extensive DNA hypermethylation within four weeks genomewidely, and repression of TET2 was one of the molecular mechanisms for DNA methylation induction.
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