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Identification of modifiers of moyamoya disease causing gene RNF213 and development of in vitro and in vivo models of the disease

Research Project

Project/Area Number 16H05437
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurosurgery
Research InstitutionKyoto University

Principal Investigator

Miyamoto Susumu  京都大学, 医学研究科, 教授 (70239440)

Co-Investigator(Kenkyū-buntansha) 高橋 淳  京都大学, iPS細胞研究所, 教授 (10270779)
高木 康志  徳島大学, 大学院医歯薬学研究部(医学域), 教授 (40312227)
小泉 昭夫  京都大学, 医学研究科, 名誉教授 (50124574)
峰晴 陽平  京都大学, 医学研究科, 助教 (50716602)
Research Collaborator KATAOKA hiroharu  
FUNAKI takashi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords脳血管障害 / もやもや病 / RNF213 / 脳血管障害学 / 脳卒中
Outline of Final Research Achievements

We aimed to identify genetic and epigenetic factors for moyamoya disease other than RNF213. We also tried to establish in vitro and in vivo models of moyamoya disease using iPS cells and R4810K knock-in mice. Whole genome sequencing in patients with moyamoya disease without the R4810K mutation identified several candidate genes, and whole genome methylation analysis of familial moyamoya disease with discordant phenotypes identified disease associated epigenetic changes. Both endothelial cells and vascular smooth muscle cells were differentiated from patient-derived iPS cells. These cells will be used to make an in vitro model of moyamoya disease for drug screening. We created both knock-out and knock-in (R4810K) mice, which will be used to develop an animal model of moyamoya disease.

Academic Significance and Societal Importance of the Research Achievements

脳血管疾患は要介護の原因の約3割を占め、超高齢化社会を迎える我が国において重要な疾患である。脳血管疾患の半分は脳梗塞であり、動脈硬化によって生じるアテローム血栓性脳梗塞に関しては研究が進んでいるが、動脈硬化以外に原因でおこる脳血管狭窄については、分子メカニズムに不明な点が多く、新規治療開発の妨げとなってきた。本研究は我が国における代表的な非動脈硬化性脳血管狭窄であるもやもや病の感受性遺伝子として同定されたRNF213の生体内での役割を明らかにし、RNF213以外の遺伝因子や環境因子を解明する端緒となる研究で、同疾患の成因の解明や新規治療法の開発につながるものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016

All Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) Book (1 results)

  • [Journal Article] Restoration of periventricular vasculature after direct bypass for moyamoya disease: intra-individual comparison2019

    • Author(s)
      Miyakoshi Akinori、Funaki Takeshi、Takahashi Jun C、Takagi Yasushi、Kikuchi Takayuki、Yoshida Kazumichi、Kataoka Hiroharu、Mineharu Yohei、Okawa Masakazu、Yamao Yukihiro、Fushimi Yasutaka、Okada Tomohisa、Togashi Kaori、Miyamoto Susumu
    • Journal Title

      Acta Neurochirurgica

      Volume: Epub aheda of print Issue: 5 Pages: 947-954

    • DOI

      10.1007/s00701-019-03866-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Moyamoya Disease in Pregnancy: A 20-Year Single-Center Experience and Literature Review2019

    • Author(s)
      Inayama Yoshihide、Kondoh Eiji、Chigusa Yoshitsugu、Io Shingo、Funaki Takeshi、Matsumura Noriomi、Miyamoto Susumu、Mandai Masaki
    • Journal Title

      World Neurosurgery

      Volume: 122 Pages: 684-691.e2

    • DOI

      10.1016/j.wneu.2018.10.071

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dysregulation of RNF213 promotes cerebral hypoperfusion2018

    • Author(s)
      Morimoto T, Enmi J, Hattori Y, Iguchi S, Saito S, Harada K, Okuda H, Mineharu Y, Takagi Y, Youssefian S, Iida H, Miyamoto S, Ihara M, Kobayashi H, Koizumi A.
    • Journal Title

      Sci Rep

      Volume: 8 Issue: 1 Pages: 3607-3607

    • DOI

      10.1038/s41598-018-22064-8

    • NAID

      120006510289

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 小児もやもや病におけるRNF213 遺伝子型の特徴2018

    • Author(s)
      峰晴陽平、宮本享
    • Journal Title

      Mt. Fuji Workshop on CVD

      Volume: 36 Pages: 20-22

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Late Cerebrovascular Events and Social Outcome after Adolescence: Long-term Outcome of Pediatric Moyamoya Disease2018

    • Author(s)
      FUNAKI Takeshi、TAKAHASHI Jun C.、MIYAMOTO Susumu
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 58 Issue: 6 Pages: 240-246

    • DOI

      10.2176/nmc.ra.2018-0026

    • NAID

      130007397185

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Magnetic resonance angiography with compressed sensing: An evaluation of moyamoya disease2018

    • Author(s)
      Yamamoto T, Okada T, Fushimi Y, Yamamoto A, Fujimoto K, Okuchi S, Fukutomi H, Takahashi JC, Funaki T, Miyamoto S, Stalder AF, Natsuaki Y, Speier P, Togashi K
    • Journal Title

      PLoS One

      Volume: 13 Issue: 1 Pages: e0189493-e0189493

    • DOI

      10.1371/journal.pone.0189493

    • NAID

      120006460064

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] RNF213 p.R4810K Variant and Intracranial Arterial Stenosis or Occlusion in Relatives of Patients with Moyamoya Disease2017

    • Author(s)
      Matsuda Yoshiko、Mineharu Yohei、Kimura Mitsuru、Takagi Yasushi、Kobayashi Hatasu、Hitomi Toshiaki、Harada Kouji H.、Uchihashi Yoshito、Funaki Takeshi、Miyamoto Susumu、Koizumi Akio
    • Journal Title

      Journal of Stroke and Cerebrovascular Diseases

      Volume: 26 Issue: 8 Pages: 1841-1847

    • DOI

      10.1016/j.jstrokecerebrovasdis.2017.04.019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significant association of RNF213 p.R4810K, a moyamoya susceptibility variant, with coronary artery disease2017

    • Author(s)
      Morimoto T, Mineharu Y, Ono K, Nakatochi M, Ichihara S, Kabata R, Takagi Y, Cao Y, Zhao L, Kobayashi H, Harada K. H, Takenaka K, Funaki T, Yokota M, Matsubara T, Yamamoto K, Izawa H, Kimura T, Miyamoto S, Koizumi A
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 4 Pages: e0175649-e0175649

    • DOI

      10.1371/journal.pone.0175649

    • NAID

      120006534994

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rapid contralateral progression of focal cerebral arteriopathy distinguished from RNF213-related moyamoya disease and fibromuscular dysplasia2017

    • Author(s)
      Araki Yoshio、Takagi Yasushi、Mineharu Yohei、Kobayashi Hatasu、Miyamoto Susumu、Wakabayashi Toshihiko
    • Journal Title

      Child's Nervous System

      Volume: 33 Issue: 8 Pages: 1405-1409

    • DOI

      10.1007/s00381-017-3451-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Angiographic features of hemorrhagic moyamoya disease with high recurrence risk: a supplementary analysis of the Japan Adult Moyamoya Trial.2017

    • Author(s)
      Funaki T, Takahashi JC, Houkin K, Kuroda S, Takeuchi S, Fujimura M, Tomata Y, Miyamoto S; on behalf of the JAM Trial Investigators
    • Journal Title

      J Neurosurg

      Volume: 14 Issue: 3 Pages: 1-8

    • DOI

      10.3171/2016.11.jns161650

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reversible striatal hypermetabolism in chorea associated with moyamoya disease: a report of two cases2016

    • Author(s)
      Sugita Y, Funaki T, Takahashi JC, Takagi Y, Fushimi Y, Kikuchi T, Yoshida K, Hatano T, Sasaki N, Miyamoto S.
    • Journal Title

      Childs Nerv Syst

      Volume: 32(11) Issue: 11 Pages: 2243-2247

    • DOI

      10.1007/s00381-016-3111-5

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significant Association of the RNF213 p.R4810K Polymorphism with Quasi-Moyamoya Disease2016

    • Author(s)
      Morimoto T, Mineharu Y, Kobayashi H, Harada KH, Funaki T, Takagi Y, Sakai N, Miyamoto S, Koizumi A
    • Journal Title

      J Stroke Cerebrovasc Dis

      Volume: 25(11) Issue: 11 Pages: 2632-2636

    • DOI

      10.1016/j.jstrokecerebrovasdis.2016.07.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of neuroendoscopic ventricular irrigation for ventriculitis2016

    • Author(s)
      Terada Y, Mineharu Y, Arakawa Y, Funaki T, Tanji M, Miyamoto S
    • Journal Title

      Clin Neurol Neurosurg

      Volume: 146 Pages: 147-151

    • DOI

      10.1016/j.clineuro.2016.05.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histopathological Characteristics of Distal Middle Cerebral Artery in Adult and Pediatric Patients with Moyamoya Disease2016

    • Author(s)
      Takagi Y, Hermanto Y, Takahashi JC, Funaki T, Kikuchi T, Mineharu Y, Yoshida K, Miyamoto S
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 56 Issue: 6 Pages: 345-349

    • DOI

      10.2176/nmc.oa.2016-0031

    • NAID

      130005157729

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histopathological Features of Brain Arteriovenous Malformations in Japanese Patients2016

    • Author(s)
      Hermanto Y, Takagi Y, Yoshida K, Ishii A, Kikuchi T, Funaki T, Mineharu Y, Miyamoto S.
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 56 Issue: 6 Pages: 340-344

    • DOI

      10.2176/nmc.oa.2016-0032

    • NAID

      130005157730

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Voxel Based Analysis of Surgical Revascularization for Moyamoya Disease: Pre- and Postoperative SPECT Studies.2016

    • Author(s)
      Fushimi Y, Okada T, Takagi Y, Funaki T, Takahashi JC, Miyamoto S, Togashi K.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 2 Pages: e0148925-e0148925

    • DOI

      10.1371/journal.pone.0148925

    • NAID

      120005763395

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] もやもや病感受性遺伝子RNF213と冠動脈疾患との関連2018

    • Author(s)
      森本貴昭
    • Organizer
      第43回日本脳卒中学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脳卒中診療におけるゲノム医療の発展と限界2018

    • Author(s)
      峰晴陽平
    • Organizer
      第77回日本脳神経外科学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Insights into pathophysiology of moyamoya disease through RNF213 and other related genes2018

    • Author(s)
      峰晴陽平
    • Organizer
      第77回日本脳神経外科学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] もやもや病感受性遺伝子RNF213の臨床における意義2018

    • Author(s)
      峰晴陽平
    • Organizer
      第43回日本脳卒中学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Significance of RNF213 for the diagnosis of moyamoya disease and related disorders2017

    • Author(s)
      Mineharu Y
    • Organizer
      第76回日本脳神経外科学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小児もやもや病におけるRNF213遺伝子型の特徴2017

    • Author(s)
      峰晴陽平
    • Organizer
      Mt. Fuji Workshop on CVD
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脳動脈瘤の遺伝子解析は血管障害の臨床と研究に如何に貢献するか2017

    • Author(s)
      峰晴陽平
    • Organizer
      第49回日本動脈硬化学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Book] Moyamoya Disease Explored Through RNF213: Genetics, Molecular Pathology, and Clinical Sciences (Current Topics in Environmental Health and Preventive Medicine)2017

    • Author(s)
      Mineharu Y, Takagi Y, Miyamoto S
    • Publisher
      Springer
    • ISBN
      9789811027109
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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