| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
The self-renewal capability of inner ear hair cells in mammals is extremely poor, thus stem cells harboring an ability to generate inner ear hair cells is a promising treatment option for damaged inner ear hair cells. We have successfully developed a new method for in vitro production of inner hair cells from pluripotent stem cells. In the present study, the mechanism of differentiation and possibility of cell transplantation using isolated cochlea tissue samples were investigated. The results showed that secretory factors related to resident cells in the inner ear facilitated differentiation of stem cells into inner ear hair cells, with several candidates determined based on RNA-sequence analysis. In addition, analysis of the co-culture with embryonic stem cell-derived inner ear hair cells and isolated cochlea tissues revealed cell settlement in the cochlea, suggesting the applicability of this in vivo method for cell transplantation therapy.
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