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Development of innovative wound care using applied immune regulation on chronic wounds

Research Project

Project/Area Number 16H05492
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionTohoku University

Principal Investigator

Tachi Masahiro  東北大学, 医学系研究科, 教授 (50312004)

Co-Investigator(Kenkyū-buntansha) 川上 和義  東北大学, 医学系研究科, 教授 (10253973)
菅野 恵美  東北大学, 医学系研究科, 准教授 (10431595)
丹野 寛大  東北大学, 医学系研究科, 助教 (10755664)
Co-Investigator(Renkei-kenkyūsha) KAZAMA itsurou  宮城大学, 看護学群(部), 教授 (60593978)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords創傷治癒 / ナチュラルキラー(NKT)細胞 / α-Galactosylceramide / 免疫 / NKT細胞 / 緑膿菌 / 緑膿菌感染
Outline of Final Research Achievements

In chronic wounds, the inflammatory responses are prolonged with persistent neutrophil infiltration. Recently, we showed that skin wound healing was delayed and the healing process was impaired under conditions lacking invariant natural killer T (iNKT) cells, an innate immune lymphocyte with potent immuno-regulatory activity.
In the present study, to identify the possible contribution of NKT cells activation, the effects of α-Galactosylceramide (α-GalCer) administration on wound healing, specific activator of iNKT cells, were examined. Administration of a-GalCer at the wound sites significantly promoted wound closure, granulation tissue formation, angiogenesis compared with vehicle treatment. In addition, the inflammatory cells were rapidly decreased and granulation tissue were enhanced in α-GalCer administered mice.

Academic Significance and Societal Importance of the Research Achievements

現在、慢性創傷における炎症遷延に対する治療法としては、抗菌薬などを用いた病原微生物に対する治療法が主流となっており、宿主免疫をターゲットとした治療法は存在しない。
本研究により、α-Galactosylceramide (α-GalCer)によるNKT細胞の活性化が炎症反応を速やかに収束させ、創傷治癒を促進することが明らかになり、新規治療法の可能性が高まった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (8 results) Book (3 results)

  • [Journal Article] Experimental wound ischemia does not promote Pseudomonas aeruginosa biofilm formation2018

    • Author(s)
      Emi Kanno, Hiromasa Tanno, Kenji Yamaguchi, Ayako Sasaki, Ryoko Maruyama, Masahiro Tachi
    • Journal Title

      Journal of Dermatology and Dermatologic Surgery

      Volume: 22 Issue: 2 Pages: 68-71

    • DOI

      10.4103/jdds.jdds_33_18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Invariant NKT cells promote skin wound healing by preventing a prolonged neutrophilic inflammatory response2017

    • Author(s)
      Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Aiko Suzuki, Naoyuki Takagi, Hideki Yamamoto, Keiko Ishii, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi
    • Journal Title

      Wound Repair and Regeneration

      Volume: 25(5) Issue: 5 Pages: 805-815

    • DOI

      10.1111/wrr.12588

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconsideration of Iodine in Wound Irrigation: The Effects on Pseudomonas Aeruginosa Biofilm Formation2016

    • Author(s)
      Emi Kanno, Hiromasa Tanno, Aiko Suzuki, Rina Kamimatsuno, Masahiro Tachi
    • Journal Title

      Journal of Wound Care

      Volume: 25 Issue: 6 Pages: 335-339

    • DOI

      10.12968/jowc.2016.25.6.335

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effect of interferon-γ deficiency on skin wound healing processes.2018

    • Author(s)
      Hiromasa TANNO, Emi KANNO, Ayako SASAKI, Keiko Ishii, Kazuyoshi KAWAKAMI
    • Organizer
      第47回日本免疫学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 緑膿菌接種創の治癒過程におけるNatural Killer T細胞の役割.2018

    • Author(s)
      丹野寛大,菅野恵美,佐藤すずな,霜野瑞葵,佐々木綾子,山口賢次,石井恵子,丸山良子,川上和義, 館正弘
    • Organizer
      第48回日本創傷治癒学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 緑膿菌接種創の治癒過程と菌排除におけるNKT細胞欠損の影響.2018

    • Author(s)
      丹野寛大,菅野恵美, 佐藤すずな, 山口賢次, 石井恵子, 丸山良子, 川上和義, 館正弘
    • Organizer
      第20回日本褥瘡学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] NKT細胞は好中球性炎症を制御し皮膚創傷治癒を促進する2017

    • Author(s)
      丹野寛大,菅野恵美,三浦孝行,石井恵子,中山俊憲,谷口克,丸山良子, 館正弘,川上和義
    • Organizer
      第28回日本生体防御学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 皮膚創傷治癒過程におけるNKT細胞の役割-好中球性炎症に注目して-2017

    • Author(s)
      丹野寛大,菅野恵美, 佐藤紀子, 正木愛梨,三浦考行,山口賢次, 石井恵子, 丸山良子, 川上和義, 館正弘
    • Organizer
      第47回日本創傷治癒学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] NKT細胞の活性化が創傷治癒に与える影響―α-Galactosylceramide創部投与による検証―2016

    • Author(s)
      丹野寛大,川上和義, 菅野恵美, 今村彩乃, 佐藤紀子,正木愛梨,高木尚之,石井恵子, 丸山良子, 館正弘
    • Organizer
      第46回日本創傷治癒学会
    • Place of Presentation
      東京大学伊藤国際学術研究センター(東京)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Local activation of iNKT cells by α-Galactosylceramide accelerates skin wound healing2016

    • Author(s)
      MASAKI Airi, TANNO Hiromasa, KANNO Emi, SATO Noriko, ISHII Keiko, KAWAKAMI Kazuyoshi
    • Organizer
      第45回日本免疫学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Lack of iNKT cells leads to persisted infiltration of neutrophils and delayed wound healing process in skin2016

    • Author(s)
      Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Keiko Ishii, Ryoko Maruyama, Masahiro Tachi
    • Organizer
      5th Congress of the World Union of Wound Healing Societies
    • Place of Presentation
      Firenze, Italy
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Book] 看護技術2017

    • Author(s)
      丹野寛大, 菅野恵美
    • Total Pages
      4
    • Publisher
      メヂィカルフレンド社
    • Related Report
      2017 Annual Research Report
  • [Book] フットケアと足病変治療ガイドブック2017

    • Author(s)
      菅野恵美, 館正弘
    • Total Pages
      6
    • Publisher
      医学書院
    • ISBN
      9784260030366
    • Related Report
      2017 Annual Research Report
  • [Book] 術後ケアとドレーン管理のすべて2016

    • Author(s)
      菅野恵美, 館正弘
    • Total Pages
      416
    • Publisher
      照林社
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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