Elucidation of the glycated collagen reaction in dentin and its application to next dental treatment
| Project/Area Number |
16H05517
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
Miura Jiro 大阪大学, 歯学部附属病院, 助教 (70437383)
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| Co-Investigator(Kenkyū-buntansha) |
北條 裕信 大阪大学, 蛋白質研究所, 教授 (00209214)
橋本 守 北海道大学, 情報科学研究科, 教授 (70237949)
久保 美寿穂 大阪大学, 歯学部附属病院, 医員 (60757813)
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| Research Collaborator |
Araki Tsutomu 大阪大学, 基礎工学研究科, 名誉教授
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Keywords | 糖化 / 象牙質 / 石灰化 / 老化 / 細胞障害 / コラーゲン / 2型糖尿病 / 糖尿病 / 蛍光寿命 / う蝕 |
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| Outline of Final Research Achievements |
Granular calcification is formed in the dental pulp of diabetic patients. Histopathologically, these are thought to be by-products of severe diabetes. In this study, the pathological calcification in dental pulp of Type2 diabetes model rat was evaluated using electron microscopy and immunohistochemical analysis of pentosidine, a kind of Advanced Glycation End products (AGEs) which is thought to be related calcification .The composition of pulp stone was examined with Energy Dispersive X-ray Spectoroscopy. In the type2 diabetes model rats, it was found that pathogenic calcification in pulp occurs with the high blood glucose level for 10 weeks in SDT fatty rats. Pentosidine which is a kind of AGEs strongly expresses in the pulp of diabetic rats. And granular calcification was also observed in the surrounding area of pulp stone. From these results, Glycation was related with calcification in dental pulp, suggesting that these are related to ectopic calcification in diabetic patients.
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| Academic Significance and Societal Importance of the Research Achievements |
糖化反応は、糖尿病ラットの歯髄内石灰化などから石灰化に関与することが示唆されてきた。本研究では、糖化コラーゲンにおける石灰化の影響を調べ、歯髄由来細胞を糖化コラーゲン上で培養することで有為に石灰化が誘導されることが分かった。これらの技術は、石灰化を誘導することで覆髄や骨造成といった技術に将来応用できると考えている。
また、齲蝕罹患象牙質において象牙細管に沿う形でAGEsが蓄積し、齲蝕罹患象牙質では蛍光寿命の顕著な短縮が認められたことから、コラーゲンより蛍光寿命の短いAGEsの蓄積が起こっていると考えられ、蛍光寿命測定が齲蝕領域を判断する指標として利用できることが示唆された。
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] UVA-activated riboflavin promotes collagen crosslinking to prevent root caries2019
Author(s)
Uemura, R., Miura, J., Ishimoto, T.,, Nakano, T., Hayashi, M.
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Journal Title
Scientific Reports
Volume: 9(1)
Issue: 3
Pages: 1252-1252
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of three drying methods of post space dentin bonding used in a direct resin composite core build-up method2018
Author(s)
Iwashita T, Mine A, Matsumoto M, Nakatani H, Higashi M, Kawaguchi-Uemura A, Kabetani T, Tajiri Y, Imai D, Hagino R, Miura J, Minamino T, Yatani H.
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Journal Title
Journal of Prosthodontic Research
Volume: 62
Issue: 4
Pages: 449-455
DOI
NAID
ISSN
1883-1958, 1883-9207
Related Report
Peer Reviewed
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[Journal Article] Adhesion procedure for CAD/CAM resin crown bonding: Reduction of bond strengths due to artificial saliva contamination2018
Author(s)
Kawaguchi-Uemura A, Mine A, Matsumoto M, Tajiri Y, Higashi M, Kabetani T, Hagino R, Imai D, Minamino T, Miura J, Yatani H.
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Journal Title
Journal of Prosthodontic Research
Volume: 62
Issue: 2
Pages: 177-183
DOI
NAID
ISSN
1883-1958, 1883-9207
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effectiveness of sodium hypochlorite and sulfinic acid sodium salt treatment on dentin-resin bonding: Long-term durability of one-step self-etching adhesive2017
Author(s)
Nakatani H, Mine A, Matsumoto M, Kabetani T, Kawaguchi-Uemura A, Higashi M, Tajiri Y, Imai D, Hagino R, Minamino T, Miura J, Yatani H.
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Journal Title
Dental Materials Journal
Volume: 36
Issue: 6
Pages: 842-850
DOI
NAID
ISSN
0287-4547, 1881-1361
Related Report
Peer Reviewed / Open Access
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[Journal Article] Early exfoliation of permanent tooth in patient with hypophosphatasia,2017
Author(s)
Okawa, R., Miura, J., Kokomoto, K., Kubota, T., Kitaoka, T., Ozono, K., Nakano, K
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Journal Title
Pediatric Dental Journal
Volume: 27
Pages: 173-178
Related Report
Peer Reviewed
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