Immunochemical analysis of dental metal allergy

• Project/Area Number: 16H05530
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tohoku University
• Principal Investigator: OGASAWARA KOUETSU 東北大学, 加齢医学研究所, 教授 (30323603)
• Research Collaborator: ITO KOYU
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 免疫 / アレルギー

Outline of Final Research Achievements

Metal allergy is considered to be delayed-type of allergy by T-cell dependent diseases, but its immune mechanism is not well understood.
In this study, we performed to clarify the characteristics of T cells involved in the onset of metal allergy using the our technology. We found that non-biased gene amplification plays a very important role in T cell receptor analysis. Thus, in T cell receptor analysis, it is necessary to use a non-biased gene amplification method, such as a single chain adapter ligation PCR by our technology.

Academic Significance and Societal Importance of the Research Achievements

本研究は、金属アレルギーを対象として、T細胞の特徴をT細胞受容体の観点から研究した。研究の過程で、T細胞受容体解析を行ったところ、現在、企業化もされているアダプターライゲーション法による解析では、不正確である可能性が明らかとなった。本研究の学術的意義は、T細胞受容体解析を行う際には、我々の開発した非バイアス遺伝子増幅法である、シングルチェーンアダプターライゲーションPCR法など、正確な解析法で行う必要があることを示した点である。

Research Products

• [Journal Article] Zinc ions have a potential to attenuate both Ni ion uptake and Ni ion-induced inflammation (2018)
  • Author(s): Onodera Ryo、Asakawa Sanki、Segawa Ryosuke、Mizuno Natsumi、Ogasawara Kouetsu、Hiratsuka Masahiro、Hirasawa Noriyasu
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s41598-018-21014-8
  • Peer Reviewed / Open Access
• [Journal Article] Nickel ions bind to HSP90β and enhance HIF-1α-mediated IL-8 expression (2018)
  • Author(s): Asakawa S, Onodera R, Kasai K, Kishimoto Y, Sato T, Segawa R, Mizuno N, Ogasawara K, Moriya T, Hiratsuka M, Hirasawa N
  • Journal Title: Toxicology Volume: 395 Pages: 45-53
  • DOI: 10.1016/j.tox.2018.01.006
  • Peer Reviewed
• [Journal Article] TRAV7-2*02 Expressing CD8+ T Cells Are Responsible for Palladium Allergy (2017)
  • Author(s): Takeda Yuri、Suto Yoshiko、Ito Koyu、Hashimoto Wataru、Nishiya Tadashi、Ueda Kyosuke、Narushima Takayuki、Takahashi Tetsu、Ogasawara Kouetsu
  • Journal Title: Int J Mol Sci. Volume: 18 Issue: 6 Pages: 1162-1162
  • DOI: 10.3390/ijms18061162
  • Peer Reviewed / Open Access
• [Journal Article] In vitro evaluation of Ag-containing calcium phosphates: Effectiveness of Ag-incorporated β-tricalcium phosphate (2017)
  • Author(s): O. Gokcekaya, K. Ueda, K. Ogasawara, H. Kanetaka, T. Narushima
  • Journal Title: Mater. Sci. Eng. C Volume: 75 Pages: 926-933
  • DOI: 10.1016/j.msec.2017.02.059
  • Peer Reviewed / Open Access
• [Journal Article] The potential role of Osteopontin in the maintenance of commensal bacteria homeostasis in the intestine. (2017)
  • Author(s): Ito K, Nakajima A1, Fukushima Y1, Suzuki K1, Sakamoto K, Hamazaki Y, Ogasawara K, Minato N, and Hattori M.
  • Journal Title: PLoS One. Volume: 12 Issue: 3 Pages: e0173629-e0173629
  • DOI: 10.1371/journal.pone.0173629
  • NAID: 120006488574
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cytotoxicity of natural killer cells activated through NKG2D contributes to the development of bronchiolitis obliterans in a murine heterotopic tracheal transplant model. (2017)
  • Author(s): Kawakami T, Ito K, Matsuda Y, Noda M, Sakurada A, Hoshikawa Y, Okada Y, Ogasawara K.
  • Journal Title: Am J Transplant. Volume: 印刷中 Issue: 9 Pages: 2338-2349
  • DOI: 10.1111/ajt.14257
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IFN-γ is required for cytotoxic T cell-dependent cancer genome immunoediting. (2017)
  • Author(s): Takeda K, Nakayama M, Hayakawa Y, Kojima Y, Ikeda H, Imai N, Ogasawara K, Okumura K, Thomas DM, Smyth MJ
  • Journal Title: Nat Commun. Volume: 8 Issue: 1 Pages: 14607-14607
  • DOI: 10.1038/ncomms14607
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] マウスモデルを用いた歯科金属アレルギーの分子機構 (2017)
  • Author(s): 小笠原康悦
  • Journal Title: 炎症と免疫 Volume: 25 Pages: 11-16
• [Journal Article] 金属と炎症 (2017)
  • Author(s): 小笠原康悦
  • Journal Title: 炎症と免疫 Volume: 25 Pages: 1-3
• [Journal Article] Quantitative in vivo biocompatibility of new ultralow-nickel cobalt-chromium-molybdenum alloys (2016)
  • Author(s): Yoshihiro Hagiwara, Kazuaki Sonofuchi, Yuichiro Koizumi, Akihiko Chiba, Mitsuko Kawano, Masafumi Nakayama, Kouetsu Ogasawara, Eiji Itoi
  • Journal Title: Journal of Orthopaedic Research Volume: in press Issue: 9 Pages: 1505-1513
  • DOI: 10.1002/jor.23150
  • Peer Reviewed
• [Presentation] 動物モデルを用いたパラジウムアレルギー発症の分子機構 (2018)
  • Author(s): 関智水、佐藤直毅、樋口繁仁、小笠原康悦
  • Organizer: 第60回 歯科基礎医学会学術大会
• [Presentation] 金属アレルギーを引き起こすT細胞の特定 (2017)
  • Author(s): 武田裕利、須藤佳子、佐藤直毅、樋口繁仁、高橋哲、伊藤甲雄、小笠原康悦
  • Organizer: 第71回 日本細菌学会東北支部総会
• [Presentation] Antibacterial property of visible-light active TiO2 layers formed on Ti-Au alloys by thermal oxidation (2017)
  • Author(s): Takatoshi Ueda, Shota Sado, Kyosuke Ueda, Koyu Ito, Kouetsu Ogasawara, Takayuki Mokudai, Hiroyasu Kanetaka, Yoshimi Niwano, Takayuki Narushima
  • Organizer: 28th European Conference on Biomaterials, Athens, Greece