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ABC transporter function in biofilm formation and its application for dental caries prevention

Research Project

Project/Area Number 16H05550
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontics/Pediatric dentistry
Research InstitutionOkayama University

Principal Investigator

NAKANO Michiyo  岡山大学, 医歯薬学総合研究科, 教授 (30359848)

Co-Investigator(Kenkyū-buntansha) 高島 由紀子  岡山大学, 大学病院, 講師 (30589768)
平野 慶子  岡山大学, 大学病院, 助教 (50335618)
稲葉 裕明  岡山大学, 医歯薬学総合研究科, 准教授 (70359850)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
KeywordsABCトランスポーター / 口腔内細菌 / バイオフィルム / Streptococcus mutans / 膜トランスポーター / シグナル伝達システム / 齲蝕 / 歯周病 / Porphyromonas gingivalis / トランスポーター / 微生物 / プロテオーム
Outline of Final Research Achievements

Streptococcus mutans possesses a large number of transporters with import/export functions, including the PII protein family that is widely distributed among bacterial species. We constructed and examined a glutamine ABC transporter (GlnP) deficient mutant strain. Biofilm formed by GlnP-deficient mutant strain was remarkably different as compared to that produced by the parental strain. GlnP-deficient mutant strain also showed a reduced number of imported molecules and lower acid tolerance ability. The S. mutans signaling system is mediated by the competence-stimulating peptide (CSP), whose precursor is encoded by the comC gene. We analyzed nrgA gene expression regulation by CSP and found that GlnP and PII protein gene expressions were decreased in comC-deficient mutant strains. Together, these results suggest that GlnP is associated with the S. mutans substrate transport and signaling system, and involved in biofilm formation.

Academic Significance and Societal Importance of the Research Achievements

ABCトランスポーターはバイオフィルム形成能に関係するだけでなく、その構造にも影響を及ぼすことはシグナル伝達システムによってこれらのタンパクの発現が制御されている可能性を示している。さらに、S. mutansの代表的なシグナル伝達システムである2成分調節因子システムにおけるcomC 欠失変異株において、これらのタンパクをコードする遺伝子の発現の低下が認められたことはこれを裏付ける結果となっている。これらの結果は、ABCトランスポーターが膜輸送の機能を有すると同時に、バイオフィルム形成におけるシグナル伝達システムに関連しており、その形成に重要な役割を果たしていることが明らかとなった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (18 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (15 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] Role of Streptococcus mutans surface proteins for biofilm formation.2018

    • Author(s)
      Matsumoto-Nakano M
    • Journal Title

      Japanese Dental Science Reviews

      Volume: 54 (1) Issue: 1 Pages: 22-29

    • DOI

      10.1016/j.jdsr.2017.08.002

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell cycle arrest and apoptosis induced by Porphyromonas gingivalis requires JNK- and p53-mediated p38 activation in human trophoblasts2018

    • Author(s)
      Hiroaki Inaba, Atsuo Amano, Richard J. Lamont, Yukitaka Murakami, Michiyo Matsumoto-Nakano.
    • Journal Title

      Infection and Immunity

      Volume: in press Issue: 4

    • DOI

      10.1128/iai.00923-17

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cariogenic properties of Streptococcus mutans clinical isolates with sortase defects.2017

    • Author(s)
      Lapirattanakul J, Takashima Y, Tantivitayakul P, Maudcheingka T, Leelataweewud P, Nakano K, Matsumoto-Nakano M.
    • Journal Title

      Archives of Oral Biology

      Volume: 81 Pages: 7-14

    • DOI

      10.1016/j.archoralbio.2017.04.018

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Streptococcus mutansのバイオフィルム形成におけるDnaKの影響と役割2018

    • Author(s)
      吉田衣里, 松三友紀, 森川優子, 仲 周平, 高島由紀子, 仲野道代
    • Organizer
      第56回日本小児歯科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Porphyromonas glae LPSによる歯肉上皮細胞の炎症性反応の評価2018

    • Author(s)
      吉田 翔, 稲葉 裕明, Alam Urmi Saki, 野村 良太, 仲野 和彦, 仲野 道代
    • Organizer
      第56回日本小児歯科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Porphyromonas gulaeが産生するプロテアーゼに関する検討2018

    • Author(s)
      Alam Urmi Saki, 吉田 翔, 稲葉 裕明, 野村 良太, 仲野 和彦, 仲野 道代
    • Organizer
      第56回日本小児歯科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乳酸菌由来のバクテリオシンがStreptococcus mutansのバイオフィルム形成に与える影響2018

    • Author(s)
      森本節代, 森川優子, 高島由紀子, 仲周平, 稲葉裕明, 仲野道代
    • Organizer
      第56回日本小児歯科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Role of molecular chaperon DnaK in Streptococcus mutans biofilm formation2018

    • Author(s)
      Matsumoto-Nakano M, Yoshida E, Matsumi Y, Takashima Y
    • Organizer
      第96回国際歯科研究学会(IADR)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Porphyromonas gulae線毛の遺伝子多型と歯肉上皮細胞への侵入能2018

    • Author(s)
      稲葉裕明, 吉田 翔, 仲野和彦, 野村良太, 仲野道代
    • Organizer
      第60回歯科基礎医学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Porphyromonas gulae LPS activates inflammatory responses in gingival epithelial cells2018

    • Author(s)
      Yoshida S, Inaba H, Nomura R, Nakano K, Matsumoto-Nakano M
    • Organizer
      第66回国際歯科研究学会日本部会総会・学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Expression of molecular chaperon DnaK on biological functions in Streptococcus mutans2017

    • Author(s)
      E. Yoshida, Y. Matsumi, Y. Takashima, and M. Matsumoto-Nakano
    • Organizer
      95th General Session & Exhibition of the IADR
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interactions between Lactobacillus strains and Streptococcus mutans for biofilm formation2017

    • Author(s)
      S. Morimoto, Y. Takashima, and M. Matsumoto-Nakano
    • Organizer
      95th General Session & Exhibition of the IADR
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Streptococcus mutansにおける分子シャペロンDnaKの役割2017

    • Author(s)
      吉田衣里、松三友紀、高島由紀子、仲野道代
    • Organizer
      第55回日本小児歯科学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小児口腔より分離されたラクトバシラスがStreptococcus mutansのバイフォフィルム形成に与える影響2017

    • Author(s)
      森本節代、高島由紀子、仲野道代
    • Organizer
      第55回日本小児歯科学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 乳酸菌が産生するバクテリオシンが口腔バイフォイルム形成に与える影響2017

    • Author(s)
      森本節代、森川優子、高島由紀子、仲周平、稲葉裕明、仲野道代
    • Organizer
      第36回日本小児歯科学会中四国地方会大会および総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional analysis of role of glutamine metabolism-related gene in biofilm formation by Streptococcus mutans2016

    • Author(s)
      Yuko Morikawa, Yukiko Takashima, Kazuyo Fujita, Michiyo Matsumoto-Nakano
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Collagen-binding property of Lactobacillus strains species isolated from Japanese children2016

    • Author(s)
      S. Morimoto, K. Fujita, and M. Matsumoto-Nakano
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of molecular chaperon DnaK on Biolfilm formation by Streptococcus mutans2016

    • Author(s)
      E. Yoshida, Y. Matsumi, Y. Takashima, and M. Matsumoto-Nakano
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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