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Uncovering the establishment process of the current distribution pattern, diversity, and adaptive genes of Chara braunii, especially in East Asia

Research Project

Project/Area Number 16H05764
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Biodiversity/Systematics
Research InstitutionKobe University

Principal Investigator

Sakayama Hidetoshi  神戸大学, 理学研究科, 准教授 (60391108)

Co-Investigator(Kenkyū-buntansha) 西山 智明  金沢大学, 学際科学実験センター, 助教 (50390688)
土松 隆志  千葉大学, 大学院理学研究院, 准教授 (60740107)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Keywordsシャジクモ / 淡水藻類 / 進化 / 種分化 / 遺伝的多様性 / 生態的多型 / 集団構造 / 生物多様性 / ゲノム / 生態型 / 藻類
Outline of Final Research Achievements

Chara braunii is distributed worldwide, excluding Antarctica, grows in a variety of freshwater environments, and exhibits high morphological variability. The current distribution pattern of C. braunii is thought to be influenced mainly by dispersal by migratory waterfowl and the creation of secondary environments (e.g., rice fields and artificial ponds for irrigation) by rice farming that began mainly in East Asia, which has created new niches for this species. In C. braunii, however, the process of establishment of the current distribution pattern and the evolutionary process of regional variation, mainly in East Asia, have not been clarified. In this study, we collected C. braunii from overseas, mainly from East Asia, and analyzed the genetic diversity, phylogenetic relationships, and phenotypes within this species to clarify the establishment process of the current distribution pattern and diversity of C. braunii.

Academic Significance and Societal Importance of the Research Achievements

本研究の研究対象であるシャジクモは、湖、ため池、水田など、わたしたちにとって身近な淡水生態系に生育する大型藻類である。しかし、日本では近年の淡水生態系破壊の結果、急速にシャジクモの衰退が進行している。その結果、シヤジクモは環境省版レッドリストにおいて絶滅危惧種として掲載されている。本研究で明らかとなった東アジアを中心に分布するシャジクモの現在の分布様式の成立過程と多様性に関する知見は、生態学的に重要な広域分布種や絶滅の危機に瀕している種の実体解明に向けた研究や施策を考える上で非常に有益な情報となり得ると期待される。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (58 results)

All 2022 2021 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (15 results) Journal Article (11 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 11 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (32 results) (of which Int'l Joint Research: 10 results,  Invited: 4 results)

  • [Int'l Joint Research] University of Wollongong(オーストラリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Sciences/Shanxi University(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Gregor Mendel Institute(オーストリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Wollongong(オーストラリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Sciences/Shanxi University(中国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Gregor Mendel Institute(オーストリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Wollongong/Royal Botanic Gardens, Melbourne(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Sciences/Shanxi University(中国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Khon Kaen University(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Gregor Mendel Institute(オーストリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Wollongong(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Gregor Mendel Institute(オーストリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Khon Kaen University(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Sciences(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] L.N.Gumilyov ENU(カザフスタン)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Discovery of ultrafast myosin, its amino acid sequence, and structural features2022

    • Author(s)
      Haraguchi Takeshi、Tamanaha Masanori、Suzuki Kano、Yoshimura Kohei、Imi Takuma、Tominaga Motoki、Sakayama Hidetoshi、Nishiyama Tomoaki、Murata Takeshi、Ito Kohji
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 8

    • DOI

      10.1073/pnas.2120962119

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 絶滅のおそれのある野生生物の選定・評価検討 -レッドリスト2020藻類-2022

    • Author(s)
      田中次郎,北山太樹,坂山英俊,寺田竜太,神谷充伸,嶌田 智
    • Journal Title

      藻類

      Volume: 70 Pages: 29-37

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphology, taxonomy and phylogenetic positions of two Spirogyra species (Zygnematophyceae, Streptophyta) with replicate and semi-replicate transverse walls from Japan2022

    • Author(s)
      Takano, T., Ikegaya, H., Ikeda, K., Nozaki, H. and Sakayama, H.
    • Journal Title

      Phycologia

      Volume: in press

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New distributional records, taxonomy, morphology, and genetic variations of the endangered brackish-water species Lamprothamnium succinctum (Charales, Charophyceae) in Japan2021

    • Author(s)
      Kato, S., Tanaka, J., Tanaka, N., Yokoyama, J., Ito, Y., Fujiwara, Y., Higa, A., Kobayashi, S., Watanabe, M. M. and Sakayama, H.
    • Journal Title

      Journal of Asia-Pacific Biodiversity

      Volume: 14 Issue: 1 Pages: 15-22

    • DOI

      10.1016/j.japb.2020.09.005

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induction of sexual reproduction reveals the presence of heterothallic Spirogyra strains (Zygnematophyceae, Streptophyta)2020

    • Author(s)
      Takano Tomoyuki、Ikegaya Hisato、Nozaki Hisayoshi、Sakayama Hidetoshi
    • Journal Title

      Phycological Research

      Volume: 68 Issue: 4 Pages: 263-268

    • DOI

      10.1111/pre.12436

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Chara Genome: Secondary Complexity and Implications for Plant Terrestrialization2018

    • Author(s)
      Nishiyama, T., Sakayama, H., de Vries, J., Buschmann, H., Saint-Marcoux, D., et al. (他55名)
    • Journal Title

      Cell

      Volume: 174 Issue: 2 Pages: 448-464

    • DOI

      10.1016/j.cell.2018.06.033

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants2018

    • Author(s)
      Higo, A., Kawashima, T., Borg, M., Zhao, M., Lopez-Vidriero, I., Sakayama, H., et al. (他18名)
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 5283-5283

    • DOI

      10.1038/s41467-018-07728-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Loss of heterophylly in aquatic plants: not ABA-mediated stress but exogenous ABA treatment induces stomatal leaves in Potamogeton perfoliatus2016

    • Author(s)
      Iida, S., Ikeda, M., Amano, M., Sakayama, H., Kadono, Y. and Kosuge, K.
    • Journal Title

      Journal of Plant Research

      Volume: 129 Issue: 5 Pages: 853-862

    • DOI

      10.1007/s10265-016-0844-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diversification of a transcription factor family led to the evolution of antagonistically acting genetic regulators of root hair growth2016

    • Author(s)
      Breuninger, H., Thamm, A., Streubel, S., Sakayama, H., Nishiyama, T. and Dolan, L.
    • Journal Title

      Current Biology

      Volume: 26 Issue: 12 Pages: 1622-1628

    • DOI

      10.1016/j.cub.2016.04.060

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Next-generation sequencing of an 88-year-old specimen of the poorly known species Liagora japonica (Nemaliales, Rhodophyta) supports the recognition of Otohimella gen. nov.2016

    • Author(s)
      Suzuki, M., Segawa, T. Mori, H., Akiyoshi, A., Ootsuki, R., Kurihara, A., Sakayama, H., Kitayama, T., Abe, T., Kogame, K., Kawai, H., Nozaki, H.
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 7 Pages: e0158944-e0158944

    • DOI

      10.1371/journal.pone.0158944

    • NAID

      120005844143

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system.2016

    • Author(s)
      Kasahara, M., Suetsugu, N., Urano, Y., Yamamoto, C., Ohmori, M., Takada, Y., Okuda, S., Nishiyama, T., Sakayama, H., Kohchi, T., Takahashi, F.
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 39232-39232

    • DOI

      10.1038/srep39232

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 日本産アオミドロ類(ストレプト植物門・ホシミドロ藻綱)の形態比較及び分子系統解析2022

    • Author(s)
      高野智之・野崎久義・坂山英俊
    • Organizer
      日本植物分類学会第21回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アオミドロ類(ストレプト植物門・ホシミドロ藻綱)の葉緑体ゲノム解読に基づく分子系統解析及び一未記載種2022

    • Author(s)
      高野智之・野崎久義・坂山英俊
    • Organizer
      日本藻類学会第46回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新規培養株を用いた接合藻類アオミドロ属及びシロゴニウム属の分子系統と形態比較2021

    • Author(s)
      高野 智之・野崎 久義・坂山 英俊
    • Organizer
      日本植物分類学会第20回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 兵庫県及び京都府から発見された接合藻類アオミドロ属における一未記載種2021

    • Author(s)
      高野 智之・野崎 久義・坂山 英俊
    • Organizer
      日本藻類学会第45回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アオミドロ類(接合藻類ストレプト植物門)の接合形態比較及び分子系統解析2021

    • Author(s)
      高野 智之・野崎 久義・坂山 英俊
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ストレプト植物RAF様キナーゼの機能解析2021

    • Author(s)
      山崎 優斗・Akida Jahan・Mousona Islam・坂山 英俊・西山 智明・堀 孝一・太田 啓之・ 皆川 純・篠澤 章久・坂田 洋一・竹澤 大輔
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 接合藻類アオミドロ属の日本新産種Spirogyra unduliseptumの分類学的再検討2020

    • Author(s)
      高野 智之・野崎 久義・坂山 英俊
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 車軸藻類Nitella sonderiとその近縁種の分類学的再検討2020

    • Author(s)
      谷口ゆりの・Adriana Garcia・坂山 英俊
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The genome and ecological evolution of Chara braunii (Charophyceae, Streptophyta)2019

    • Author(s)
      Sakayama, H. and Nishiyama, T.
    • Organizer
      The 5th International Volvox Conference, Tokyo
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] First discovery of heterothallic sexuality in the genus Spirogyra using culture strains2019

    • Author(s)
      Takano, T. and Sakayama, H.
    • Organizer
      The 5th International Volvox Conference, Tokyo
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 車軸藻類シャジクモ(Chara braunii)とその近縁種の分類学的再検討2019

    • Author(s)
      坂山 英俊・玉置 千紘・Adriana Garcia・Zhongmin Sun・川井 浩史・西山 智明
    • Organizer
      日本植物学会第83回大会、東北大学(仙台)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 接合藻類アオミドロ属の西日本産を中心とした新規株の系統解析2019

    • Author(s)
      高野 智之・野崎 久義・坂山 英俊
    • Organizer
      日本植物学会第83回大会、東北大学(仙台)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Progress in Streptophyte Algae Genomics and Genetics2019

    • Author(s)
      Nishiyama, T., Kamada, H., Kasahara, M., Sakayama, H., Tsuchikane, Y. and Sekimoto, H.
    • Organizer
      Plant and Animal Genome XXVII Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シャジクモ(Chara)属 Charopsis 節の分類学的再検討2019

    • Author(s)
      玉置千紘・Adriana Garcia・Zhongmin Sun・Wuttipong Mahakham・川井浩史・坂山英俊
    • Organizer
      日本藻類学会第43回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The genome and ecological evolution of a cosmopolitan freshwater alga Chara braunii (Charales, Streptophyta)2018

    • Author(s)
      Sakayama, H., Kato, S. and Nishiyama, T.
    • Organizer
      East Asian Plant Diversity and Conservation 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シャジクモ細胞膜リン酸輸送体の分子機能解析2018

    • Author(s)
      三村徹郎・大西美輪・村西直樹・藤原ひとみ・石崎公庸・深城英弘・西山智明・坂山英俊・Reid Rob J.・且原真木
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] NIMA関連キナーゼファミリーによる細胞伸長制御の進化的な保存性2018

    • Author(s)
      橋爪駿・高谷彰吾・日渡祐二・坂山英俊・西山智明・髙橋卓・本瀬宏康
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シャジクモの生態型の進化に関する研究2017

    • Author(s)
      坂山英俊・宮田大輔・加藤 将・西山智明
    • Organizer
      日本藻類学会第41回大会
    • Place of Presentation
      高知大学(高知)
    • Year and Date
      2017-03-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 富士五湖における最近の車軸藻類の分布状況2017

    • Author(s)
      上嶋崇嗣・中村誠司・加藤 将・坂山英俊・芹澤(松山)和世・芹澤如比古
    • Organizer
      日本藻類学会第41回大会
    • Place of Presentation
      高知大学(高知)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 分離集団の薄いショットガンシーケンシングによるシャジクモ遺伝学的地図の構築2017

    • Author(s)
      西山智明・鎌田寛彬・宮田大輔・山口勝司・重信秀治・坂山英俊・笠原雅弘
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 精子を持つ植物で見つかったアデニル酸シクラーゼ2017

    • Author(s)
      笠原賢洋・末次憲之・浦野裕貴・山本千愛・大森未樹矢・高田侑季・奥田修二郎・西山智明・坂山英俊・河内 孝之・高橋文雄
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] De novo sequencing of Chara braunii genome and construction of genetic map2017

    • Author(s)
      Nishiyama, T., Kamada, H., Miyata, D., Kasahara, M. and Sakayama, H.
    • Organizer
      Advanced Genome Science International Symposium, The Start of New Genomics
    • Place of Presentation
      伊藤国際学術研究センター(東京)
    • Year and Date
      2017-01-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シャジクモの生殖器官のトランスクリプ トーム解析2016

    • Author(s)
      西山智明・坂山英俊
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] シャジクモの発生遺伝子の発現プロファイリングに関する研究2016

    • Author(s)
      渡邊みゆき・西山智明・鈴木 穣・坂山英俊
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 速度改変型ミオシン XI 導入による単子葉植物ブラキポ ディウムの成長促進2016

    • Author(s)
      原口武士・木下佳菜・玉那覇正典・坂山英俊・西山智明・富永基樹・伊藤光二
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生物界最速ミオシンである Chara braunii (シャジクモ)のミオシンXIの機能解析2016

    • Author(s)
      玉那覇正典・原口武士・木下佳菜1・坂山英俊・西山智明・富永基樹・伊藤光二
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 藻類・陸上植物の細胞壁空間に ROS を産生する酵素 Rboh の分子進化2016

    • Author(s)
      板橋武・橋本研志・坂山英俊・西山智明・北畑信隆・Bonnot Clemence・Hetherington Sandy・Dolan Liam・朽津和幸
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genome and transcriptome of charophytes2016

    • Author(s)
      Sakayama, H. & Nishiyama, T.
    • Organizer
      7th International Research Group on Charophytes Symposium
    • Place of Presentation
      Astana, Kazakhstan
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Charophytes, origin of land plants and terminology2016

    • Author(s)
      Garcia, A. & Sakayama, H.
    • Organizer
      7th International Research Group on Charophytes Symposium
    • Place of Presentation
      Astana, Kazakhstan
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genomes of bryophytes and charophyceans2016

    • Author(s)
      Nishiyama, T., Sakayama, H, & Sakakibara, K.
    • Organizer
      ICAR 2016 International Conference on Arabidopsis Research
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2016-06-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Evolutionary analyses on ecological differentiation of a cosmopolitan freshwater alga Chara braunii (Charales, Streptophyta)2016

    • Author(s)
      Sakayama, H., Miyata, D., Kato, S. & Nishiyama, T.
    • Organizer
      EMBO Workshop, New model systems for early land plant evolution
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome sequencing charophyceans, liverworts, and hornworts: making model systems2016

    • Author(s)
      Nishiyama, T., Sakayama, H, Sakakibara, K, et al.
    • Organizer
      EMBO Workshop, New model systems for early land plant evolution
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2023-01-30  

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