Analysis of genetic polymorphism of poultry red mites in the world and search for candidate molecules of anti-mite vaccines

• Project/Area Number: 16H05804
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Kazuhiko 北海道大学, 獣医学研究院, 教授 (90250498)
• Co-Investigator(Kenkyū-buntansha): 村田 史郎 北海道大学, 獣医学研究院, 助教 (10579163) ; 今内 覚 北海道大学, 獣医学研究院, 准教授 (40396304)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: ワクモ / フェリチン / ワクチン

Outline of Final Research Achievements

Poultry red mites (PRMs), Dermanyssus gallinae, are blood-sucking ectoparasites of chickens, which cause huge economic losses in the poultry industries worldwide. To establish a new effective control method for PRMs, we have searched for and identified candidate antigens which are not polymorphic among PRMs from different areas in the world to develop anti-PRM vaccines. Several antigens identified in this study showed anti-PRM effects determined by in vitro feeding assay using sera from immunized chickens.

Academic Significance and Societal Importance of the Research Achievements

ワクモは、世界各地の養鶏場で大きな被害をもたらしているが、その対策は、主に駆虫剤による防除に依存している。しかし薬剤耐性ワクモの出現が数多く報告され、駆虫剤の効果減少や駆虫剤残存による動物衛生環境の悪化などが問題となっており、駆虫剤以外の新規防除法の開発が急務となっている。本研究は、新規防除法としてワクモ分子を標的とした抗ワクモワクチンの可能性を提示したものであり、本研究の成果は、鶏の感染症制御に大きく貢献できると思われる。さらに本研究の手法は、他の外部寄生虫研究分野へも広く応用可能で汎用性が高く、制御困難な種々の外部寄生虫に対する防除法の開発にも大きく貢献できると考える。

Research Products

• [Journal Article] Carbohydrate Metabolic Compensation Coupled to High Tolerance to Oxidative Stress in Ticks. (2019)
  • Author(s): Della Noce B, Carvalho Uhl MV, Machado J, Waltero CF, de Abreu LA, da Silva RM, da Fonseca RN, de Barros CM, Sabadin G, Konnai S, da Silva Vaz I Jr, Ohashi K, Logullo C.
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 4753-4753
  • DOI: 10.1038/s41598-019-41036-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis (2018)
  • Author(s): Seixas A, Alzugaray MF, Tirloni L, Parizi LF, Pinto AFM, Githaka NW, Konnai S, Ohashi K, Yates JR III, Termignoni C, da Silva Vaz I Jr
  • Journal Title: Ticks and Tick-borne Diseases Volume: 9 Issue: 1 Pages: 72-81
  • DOI: 10.1016/j.ttbdis.2017.10.006
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular detection and phylogenetic analysis of Ehrlichia canis in Philippine dogs (2018)
  • Author(s): Maekawa N, Konnai S, Balbin MM, Mingala CN, Gicana KRB, Bernando FAEM, Murata S, Ohashi K
  • Journal Title: Ticks Tick Borne Dis Volume: 9(2) Issue: 2 Pages: 266-269
  • DOI: 10.1016/j.ttbdis.2017.09.012
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A novel mechanism of functional cooperativity regulation by thiol redox status in a dimeric inorganic pyrophosphatase (2017)
  • Author(s): Costa EP, Facanha AR, Cruz CS, Silva JN, Machado JA, Carvalho GM, Fernandes MR, Martins R, Campos E, Romeiro NC, Githaka NW, Konnai S, Ohashi K, Vaz IS Jr, Logullo C.
  • Journal Title: Biochim Biophys Acta. Volume: 1861(1 Pt A) Issue: 1 Pages: 2922-2933
  • DOI: 10.1016/j.bbagen.2016.09.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular and structural characterization of novel cystatins from the taiga tick Ixodes persulcatus (2017)
  • Author(s): Rangel CK, Parizi LF, Sabadin GA, Costa EP, Romeiro NC, Isezaki M, Githaka NW, Seixas A, Logullo C, Konnai S, Ohashi K, da Silva Vaz I Jr.
  • Journal Title: Ticks Tick Borne Dis. Volume: 8(3) Issue: 3 Pages: 432-441
  • DOI: 10.1016/j.ttbdis.2017.01.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ミャンマー連邦共和国における鶏の吸血性外部寄生虫の分布状況調査 (2018)
  • Author(s): 竹原昌生、村田史郎、片倉賢、Myint Myint Hmoon、Shwe Yee Win、Saw Bawm、Lat Lat Htun、Ye Htut Aung、Mar Mar Win、伊勢崎政美、 今内覚、大橋和彦
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] マレック病ウイルス弱毒株を用いた新規抗ワクモワクチン開発に向けた基礎研究 (2017)
  • Author(s): 青山珠里愛、村田史郎、伊勢崎政美、種子野章、酒井英史、今内覚、 大橋和彦
  • Organizer: 第160回日本獣医学会
• [Presentation] 抗ワクモ(Dermanyssus gallinae)ワクチン開発に向けたワクモ由来フェリチン2の性状解析 (2016)
  • Author(s): 伊勢崎 政美、村田史郎、酒井英史、矢吹卓也、種子野章、市居修、伊東拓也、今内覚、大橋和彦
  • Organizer: 第１５９回日本獣医学会
  • Place of Presentation: 日本大学生物資源科学部、藤沢市、神奈川県
  • Year and Date: 2016-09-06