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Molecular identication of Fasciola speceis distributed in Uganda and elucidation of dispersal routes

Research Project

Project/Area Number 16H05806
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Veterinary medical science
Research InstitutionIwate University

Principal Investigator

Ichikawa-Seki Madoka  岩手大学, 農学部, 助教 (20700488)

Research Collaborator Echodu Richard  
Vudriko Patrick  
Terefe Yitagele  
Abdelkarim Laatamna  
Halajian Ali  
Hakim Hakimullah  
Qamar Muhammad Fiaz  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords獣医学 / 人獣共通感染症 / 寄生虫 / 肝蛭 / 人獣共通感染性
Outline of Final Research Achievements

Fasciolosis caused by liver fluke infection has severe economic damage to the livestock industry all over the world. However, in many African countries, The endemic Fasciola species has not accurately identified. The objective of this study was to investigate endemic species by using molecular species identification markers which developed recently by our research project. In addition, dispersal route of Fasciola flukes inside African continent was analyzed by the phylogenetic markers.
Uganda was the first research center in this project, then, we expanded the target areas to north and south African countries as well as middle east area to examine the dispersal route of Fasciola flukes inside and outside the African continent.

Academic Significance and Societal Importance of the Research Achievements

研究代表者が世界に先駆けて開発した新規種識別マーカーを肝蛭症が問題となっている現場に適用し、病因学的研究に役立てた点に本研究成果の学術的意義と社会的意義を見出すことができる。
本研究では、肝蛭症による経済被害が周辺諸国に比べて深刻なウガンダにおける流行種を正確に識別し、肝蛭症の現状を把握することができた。
また、研究拠点としたウガンダとその周辺国での肝蛭の伝播経路を辿ることができた。このような本研究成果を現地の研究者・獣医師と共有したことにより、効果的な肝蛭症防除対策を考案する契機となることができたと確信する。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (26 results)

All 2018 2017 2016 Other

All Int'l Joint Research (11 results) Journal Article (9 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 9 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] Makerere University/Gulu University(ウガンダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Haramaya University(エチオピア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Djelfa(アルジェリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Limpopo(南アフリカ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Kabul University(アフガニスタン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Makerere University/Gulu University(ウガンダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Haramaya University(エチオピア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Djelfa(アルジェリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Limpopo(南アフリカ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Kabul University(アフガニスタン)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Gulu University/Makerere University(Uganda)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Hybrid origin of Asian aspermic <i>Fasciola</i> flukes is confirmed by analyzing two single-copy genes, <i>pepck</i> and <i>pold</i>2018

    • Author(s)
      Hayashi K, Ichikawa-Seki M, Mohanta UK, Shoriki T, Chaichanasak P, Itagaki T.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 80 Issue: 1 Pages: 98-102

    • DOI

      10.1292/jvms.17-0406

    • NAID

      130006321211

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Seroprevalence of Toxoplasma gondii in humans and pigs in North Sulawesi, Indonesia2017

    • Author(s)
      Tuda J, Adiani S, Ichikawa-Seki M, Umeda K, Nishikawa Y
    • Journal Title

      Parasitol Int.

      Volume: 印刷中 Issue: 5 Pages: 615-618

    • DOI

      10.1016/j.parint.2017.04.011

    • NAID

      120006402300

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evaluation of immunochromatographic test (ICT) strips for the serological detection of Babesia bovis and Babesia bigemina infection in cattle from Western Java, Indonesia2017

    • Author(s)
      Guswanto A, Allamanda P, Mariamah ES, Munkjargal T, Tuvshintulga B, Takemae H, Sivakumar T, AbouLaila M, Terkawi MA, Ichikawa-Seki M, Nishikawa Y, Yokoyama N, Igarashi I
    • Journal Title

      Vet Parasitol

      Volume: 印刷中 Pages: 76-79

    • DOI

      10.1016/j.vetpar.2017.04.010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular characterization of Fasciola flukes obtained from wild sika deer and domestic cattle in Hokkaido, Japan2017

    • Author(s)
      Ichikawa-Seki Madoka、Shiroma Tomoko、Kariya Tatsuya、Nakao Ryo、Ohari Yuma、Hayashi Kei、Fukumoto Shinya
    • Journal Title

      Parasitology International

      Volume: 66 Issue: 5 Pages: 519-521

    • DOI

      10.1016/j.parint.2017.04.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] First detection of Allobilharzia visceralis (Schistosomatidae, Trematoda) from Cygnus cygnus in Japan.2017

    • Author(s)
      Hayashi K, Ichikawa-Seki M, Ohari Y, Mohanta UK, Aita J, Satoh H, Ehara S, Tokashiki M, Shiroma T, Azuta A, Oka N, Watanabe T, Harasawa R, Inohana S, Ichijo T, Furuhama K.
    • Journal Title

      Parasitology International

      Volume: 66 Issue: 1 Pages: 925-929

    • DOI

      10.1016/j.parint.2016.10.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria.2017

    • Author(s)
      Ichikawa-Seki M, Tokashiki M, Opara MN, Iroh G, Hayashi K, Kumar UM, Itagaki T.
    • Journal Title

      Parasitology International

      Volume: 66 Issue: 1 Pages: 893-897

    • DOI

      10.1016/j.parint.2016.10.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China.2017

    • Author(s)
      Ichikawa-Seki M, Peng M, Hayashi K, Shoriki T, Mohanta UK, Shibahara T, Itagaki T.
    • Journal Title

      Parasitology

      Volume: 144 Issue: 2 Pages: 206-213

    • DOI

      10.1017/s003118201600161x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia.2016

    • Author(s)
      Hayashi K, Ichikawa-Seki M, Allamanda P, Wibowo PE, Mohanta UK, Sodirun, Guswanto A, Nishikawa Y.
    • Journal Title

      Parasitology International

      Volume: 65 Issue: 5 Pages: 424-427

    • DOI

      10.1016/j.parint.2016.06.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Reappraisal of Hydatigera taeniaeformis (Batsch, 1786) (Cestoda: Taeniidae) sensu lato with description of Hydatigera kamiyai n. sp.2016

    • Author(s)
      Lavikainen A, Iwaki T, Haukisalmi V, Konyaev SV, Casiraghi M, Dokuchaev NE, Galimberti A, Halajian A, Henttonen H, Ichikawa-Seki M, Itagaki T, Krivopalov AV, Meri S, Morand S, N&auml;reaho A, Olsson GE, Ribas A, Terefe Y, Nakao M.
    • Journal Title

      International Journal for Parasitology

      Volume: 46 Issue: 5-6 Pages: 361-374

    • DOI

      10.1016/j.ijpara.2016.01.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Development of quantitative PCRs to determine nuclear genotypes of hybrid Fasciola flukes.2018

    • Author(s)
      Tashibu, A., Hayashi, K. Nakao, M., Mohanta U.K., Fukumoto, S., Nakao, R., Ichikawa-Seki, M.
    • Organizer
      14th International Congress of Parasitology, Taegu
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立.2018

    • Author(s)
      関まどか、田渋敦士、Thang, N.T.、林慶、中尾稔、Mohanta, U.K.、福本晋也、中尾亮
    • Organizer
      第26回分子寄生虫学ワークショップ第14回分子寄生虫・マラリア研究フォーラム合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立2018

    • Author(s)
      田渋敦士、林慶、中尾稔、Mohanta, U.K.、福本晋也、中尾亮、関まどか
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular characterization and phylogenetic analysis of Fasciola flukes in sheep from Afghanistan.2018

    • Author(s)
      Thang, N.T., Hakim, H., Tashibu, A., Rahimi, R.R., Ichikawa-Seki, M.
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立.2018

    • Author(s)
      関まどか、田渋敦士、Thang N.T.、林慶、中尾稔、Mohanta, U.K.、福本晋也、中尾亮
    • Organizer
      第12回蠕虫研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular characterization and phylogenetic analysis of Fasciola flukes from Spain.2018

    • Author(s)
      Thang, N.T., Vazquez-Prieto, S., Vilas, R., Paniagua, E., Ubeira, F. M, Ichikawa-Seki, M.
    • Organizer
      第12回蠕虫研究会
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2022-10-20  

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