Molecular identication of Fasciola speceis distributed in Uganda and elucidation of dispersal routes

• Project/Area Number: 16H05806
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Iwate University
• Principal Investigator: Ichikawa-Seki Madoka 岩手大学, 農学部, 助教 (20700488)
• Research Collaborator: Echodu Richard ; Vudriko Patrick ; Terefe Yitagele ; Abdelkarim Laatamna ; Halajian Ali ; Hakim Hakimullah ; Qamar Muhammad Fiaz
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 獣医学 / 人獣共通感染症 / 寄生虫 / 肝蛭 / 人獣共通感染性

Outline of Final Research Achievements

Fasciolosis caused by liver fluke infection has severe economic damage to the livestock industry all over the world. However, in many African countries, The endemic Fasciola species has not accurately identified. The objective of this study was to investigate endemic species by using molecular species identification markers which developed recently by our research project. In addition, dispersal route of Fasciola flukes inside African continent was analyzed by the phylogenetic markers.
Uganda was the first research center in this project, then, we expanded the target areas to north and south African countries as well as middle east area to examine the dispersal route of Fasciola flukes inside and outside the African continent.

Academic Significance and Societal Importance of the Research Achievements

研究代表者が世界に先駆けて開発した新規種識別マーカーを肝蛭症が問題となっている現場に適用し、病因学的研究に役立てた点に本研究成果の学術的意義と社会的意義を見出すことができる。
本研究では、肝蛭症による経済被害が周辺諸国に比べて深刻なウガンダにおける流行種を正確に識別し、肝蛭症の現状を把握することができた。
また、研究拠点としたウガンダとその周辺国での肝蛭の伝播経路を辿ることができた。このような本研究成果を現地の研究者・獣医師と共有したことにより、効果的な肝蛭症防除対策を考案する契機となることができたと確信する。

Research Products

• [Int'l Joint Research] Makerere University/Gulu University(ウガンダ)
• [Int'l Joint Research] Haramaya University(エチオピア)
• [Int'l Joint Research] University of Djelfa(アルジェリア)
• [Int'l Joint Research] University of Limpopo(南アフリカ)
• [Int'l Joint Research] Kabul University(アフガニスタン)
• [Int'l Joint Research] Makerere University/Gulu University(ウガンダ)
• [Int'l Joint Research] Haramaya University(エチオピア)
• [Int'l Joint Research] University of Djelfa(アルジェリア)
• [Int'l Joint Research] University of Limpopo(南アフリカ)
• [Int'l Joint Research] Kabul University(アフガニスタン)
• [Int'l Joint Research] Gulu University/Makerere University(Uganda)
• [Journal Article] Hybrid origin of Asian aspermic <i>Fasciola</i> flukes is confirmed by analyzing two single-copy genes, <i>pepck</i> and <i>pold</i> (2018)
  • Author(s): Hayashi K, Ichikawa-Seki M, Mohanta UK, Shoriki T, Chaichanasak P, Itagaki T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 1 Pages: 98-102
  • DOI: 10.1292/jvms.17-0406
  • NAID: 130006321211
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seroprevalence of Toxoplasma gondii in humans and pigs in North Sulawesi, Indonesia (2017)
  • Author(s): Tuda J, Adiani S, Ichikawa-Seki M, Umeda K, Nishikawa Y
  • Journal Title: Parasitol Int. Volume: 印刷中 Issue: 5 Pages: 615-618
  • DOI: 10.1016/j.parint.2017.04.011
  • NAID: 120006402300
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of immunochromatographic test (ICT) strips for the serological detection of Babesia bovis and Babesia bigemina infection in cattle from Western Java, Indonesia (2017)
  • Author(s): Guswanto A, Allamanda P, Mariamah ES, Munkjargal T, Tuvshintulga B, Takemae H, Sivakumar T, AbouLaila M, Terkawi MA, Ichikawa-Seki M, Nishikawa Y, Yokoyama N, Igarashi I
  • Journal Title: Vet Parasitol Volume: 印刷中 Pages: 76-79
  • DOI: 10.1016/j.vetpar.2017.04.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular characterization of Fasciola flukes obtained from wild sika deer and domestic cattle in Hokkaido, Japan (2017)
  • Author(s): Ichikawa-Seki Madoka、Shiroma Tomoko、Kariya Tatsuya、Nakao Ryo、Ohari Yuma、Hayashi Kei、Fukumoto Shinya
  • Journal Title: Parasitology International Volume: 66 Issue: 5 Pages: 519-521
  • DOI: 10.1016/j.parint.2017.04.005
  • Peer Reviewed / Open Access
• [Journal Article] First detection of Allobilharzia visceralis (Schistosomatidae, Trematoda) from Cygnus cygnus in Japan. (2017)
  • Author(s): Hayashi K, Ichikawa-Seki M, Ohari Y, Mohanta UK, Aita J, Satoh H, Ehara S, Tokashiki M, Shiroma T, Azuta A, Oka N, Watanabe T, Harasawa R, Inohana S, Ichijo T, Furuhama K.
  • Journal Title: Parasitology International Volume: 66 Issue: 1 Pages: 925-929
  • DOI: 10.1016/j.parint.2016.10.015
  • Peer Reviewed
• [Journal Article] Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria. (2017)
  • Author(s): Ichikawa-Seki M, Tokashiki M, Opara MN, Iroh G, Hayashi K, Kumar UM, Itagaki T.
  • Journal Title: Parasitology International Volume: 66 Issue: 1 Pages: 893-897
  • DOI: 10.1016/j.parint.2016.10.010
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China. (2017)
  • Author(s): Ichikawa-Seki M, Peng M, Hayashi K, Shoriki T, Mohanta UK, Shibahara T, Itagaki T.
  • Journal Title: Parasitology Volume: 144 Issue: 2 Pages: 206-213
  • DOI: 10.1017/s003118201600161x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia. (2016)
  • Author(s): Hayashi K, Ichikawa-Seki M, Allamanda P, Wibowo PE, Mohanta UK, Sodirun, Guswanto A, Nishikawa Y.
  • Journal Title: Parasitology International Volume: 65 Issue: 5 Pages: 424-427
  • DOI: 10.1016/j.parint.2016.06.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reappraisal of Hydatigera taeniaeformis (Batsch, 1786) (Cestoda: Taeniidae) sensu lato with description of Hydatigera kamiyai n. sp. (2016)
  • Author(s): Lavikainen A, Iwaki T, Haukisalmi V, Konyaev SV, Casiraghi M, Dokuchaev NE, Galimberti A, Halajian A, Henttonen H, Ichikawa-Seki M, Itagaki T, Krivopalov AV, Meri S, Morand S, N&auml;reaho A, Olsson GE, Ribas A, Terefe Y, Nakao M.
  • Journal Title: International Journal for Parasitology Volume: 46 Issue: 5-6 Pages: 361-374
  • DOI: 10.1016/j.ijpara.2016.01.009
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Development of quantitative PCRs to determine nuclear genotypes of hybrid Fasciola flukes. (2018)
  • Author(s): Tashibu, A., Hayashi, K. Nakao, M., Mohanta U.K., Fukumoto, S., Nakao, R., Ichikawa-Seki, M.
  • Organizer: 14th International Congress of Parasitology, Taegu
  • Int'l Joint Research
• [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立. (2018)
  • Author(s): 関まどか、田渋敦士、Thang, N.T.、林慶、中尾稔、Mohanta, U.K.、福本晋也、中尾亮
  • Organizer: 第26回分子寄生虫学ワークショップ第14回分子寄生虫・マラリア研究フォーラム合同大会
  • Int'l Joint Research
• [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立 (2018)
  • Author(s): 田渋敦士、林慶、中尾稔、Mohanta, U.K.、福本晋也、中尾亮、関まどか
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Molecular characterization and phylogenetic analysis of Fasciola flukes in sheep from Afghanistan. (2018)
  • Author(s): Thang, N.T., Hakim, H., Tashibu, A., Rahimi, R.R., Ichikawa-Seki, M.
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立. (2018)
  • Author(s): 関まどか、田渋敦士、Thang N.T.、林慶、中尾稔、Mohanta, U.K.、福本晋也、中尾亮
  • Organizer: 第12回蠕虫研究会
• [Presentation] Molecular characterization and phylogenetic analysis of Fasciola flukes from Spain. (2018)
  • Author(s): Thang, N.T., Vazquez-Prieto, S., Vilas, R., Paniagua, E., Ubeira, F. M, Ichikawa-Seki, M.
  • Organizer: 第12回蠕虫研究会