Molecular epidemiological study on ungulate malaria parasites

• Project/Area Number: 16H05807
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Nagasaki University
• Principal Investigator: ASADA Masahito 長崎大学, 熱帯医学研究所, 助教 (40587028)
• Co-Investigator(Kenkyū-buntansha): 金子 修 長崎大学, 熱帯医学研究所, 教授 (50325370)
• Research Collaborator: KAEWTHAMASORN Morakot ; TEMPLETON Thomas ; HAKIMI Hassan ; TAKEDA Mika
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: マラリア / スイギュウ / ヤギ / 偶蹄類 / シカ / タイ / 獣医学 / 感染症 / 寄生虫 / ウシ科

Outline of Final Research Achievements

Malaria parasites of even-toed ungulate have been sparsely described after its discovery. Plasmodium bubalis was reported from water buffalo in India in 1919, and P. caprae was reported from goat in Angola in 1923, however these studies were based on microscopic description and no further epidemiological or phylogenetical studies have been conducted.
We performed PCR surveys using blood from ungulates and we found that Plasmodium is readily detectable from water buffaloes in Thailand and Vietnam. Plasmodium DNA was also detected from goats in Zambia, Sudan and Kenya in Africa, Iran in west Asia, and Myanmar and Thailand in southeast Asia. Complete mitochondrial DNA sequences were determined and used to infer a phylogeny in which ungulate malaria parasites form a monophyletic clade within the Haemosporida, and branch prior to the clade containing bird, lizard and other mammalian Plasmodium.

Academic Significance and Societal Importance of the Research Achievements

本研究から、スイギュウのマラリア並びにヤギのマラリアが広く東南アジアを含む地域で蔓延していることが明らかとなった。スイギュウ、ヤギは使役動物や乳・肉の生産を通じて現地の農畜産業を支える重要な家畜であるため、今後詳細な病原解析と偶蹄類家畜のマラリアに対する対策の構築が必要である。

Research Products

• [Journal Article] タイにおける偶蹄類マラリア原虫調査 (2018)
  • Author(s): 麻田正仁
  • Journal Title: 日本寄生虫学会 Volume: 17(2) Pages: 59-64
  • Peer Reviewed
• [Journal Article] The rediscovery of malaria parasites of ungulates (2016)
  • Author(s): Templeton TJ, Martinsen E, Kaewthamasorn M, Kaneko O.
  • Journal Title: Parasitology Volume: 143(12) Issue: 12 Pages: 1501-1508
  • DOI: 10.1017/s0031182016001141
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion with domestic goat host (2018)
  • Author(s): Kaewthamasorn M, Takeda M, Saiwichai T, Gitaka J, Tiawsirisup S, Imasato Y, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi D, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim MA, Suganuma K, Hakimi H, Nakao R, Katakura K, Asada M, Kaneko O
  • Organizer: 14th International Congress of Parasitology
  • Int'l Joint Research
• [Presentation] Close relationship of Plasmodium sequences detected from South American pampas deer (Ozotoceros bezoarticus) to Plasmodium spp. in North American white-tailed deer (2018)
  • Author(s): Takeda M, Asada M, Tomas WM, Pellegrin A, de Oliveira CHS, Barbosa JD, da Silveira JAG, Braga EM, Kaneko O
  • Organizer: The 17th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion with domestic goat host (2018)
  • Author(s): Kaewthamasorn M, Takeda M, Saiwichai T, Gitaka J, Tiawsirisup S, Imasato Y, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi D, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim MA, Suganuma K, Hakimi H, Nakao R, Katakura K, Asada M, Kaneko O
  • Organizer: The 17th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] 南米のパンパスジカ寄生マラリア原虫は北米のオジロジカ寄生マラリア原虫に近縁である (2018)
  • Author(s): 麻田正仁, 竹田美香, Tomas WM, Pellegrin A, Oliveira CHS, Barbosa JD, Silveira JAG, Braga EM, 金子修
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion withdomestic goat host (2018)
  • Author(s): 麻田正仁, Kaewthamasorn M, 竹田美香, Saiwichai T, Gitaka J, Tiawsirisup S, 今里祐平, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi DK, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim AM, 菅沼啓輔, Hakimi H, 中尾亮, 片倉賢, 金子修
  • Organizer: 第59回日本熱帯医学会大会
• [Presentation] アジア及びアフリカのヤギマラリア原虫はヤギの分布域拡大に併せて広まった (2018)
  • Author(s): Kaewthamasorn M, 竹田美香, Saiwichai T, Gitaka JN, Tiawsirisup S, 今里裕平, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi DK, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim AM, Suganuma K, Hakimi H, 中尾亮, 片倉賢, 麻田正仁, 金子修
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] 偶蹄類のマラリア原虫 (2018)
  • Author(s): 麻田正仁, 金子修
  • Organizer: 第33回生態学・疫学談話会
  • Invited
• [Presentation] Ungulate malaria parasites (2017)
  • Author(s): Templeton TJ, Asada M, Montakan J, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea Sm Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Ungulate malaria parasites (2017)
  • Author(s): Templeton TJ, Asada M, Montakan J, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea Sm Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
  • Organizer: The 13th Nagasaki-Singapore Medical Symposium / Leading Program International Symposium
  • Int'l Joint Research
• [Presentation] タイのヤギマラリア原虫 (2017)
  • Author(s): 麻田正仁、Kaewthamasorn M、竹田美香、Tiawsirisup S、Saiwichai T、Kaewlamun W、金子修
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Ungulate malaria parasites (2017)
  • Author(s): Templeton TJ, Asada M, Jiratanh M, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea S, Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
  • Organizer: Japan-Brazil Malaria Workshop
  • Int'l Joint Research
• [Presentation] Loop-Mediated Isothermal Amplification によるスイギュウマラリア原虫検出法の確立 (2016)
  • Author(s): 麻田正仁、Morakot Kaewthamasorn、竹田美香、外川裕人、 Thomas J. Templeton、金子修
  • Organizer: 第159回日本獣医学会学術集会
• [Presentation] Ungulate malaria parasites (2016)
  • Author(s): Templeton TJ, Asada M, Jiratanh M, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea S, Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
  • Organizer: 第5回感染症若手フォーラム
• [Presentation] Ungulate malaria parasites (2016)
  • Author(s): Templeton TJ, Asada M, Jiratanh M, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea S, Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
  • Organizer: The 15th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research