Ethnic difference in metabolic capacity of arsenic by AS3MT genetic polymorphisms

• Project/Area Number: 16H05887
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Prefectural University of Kumamoto
• Principal Investigator: Agusa Tetsuro 熊本県立大学, 環境共生学部, 准教授 (50403853)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000); Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2016: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
• Keywords: ヒ素 / 地下水 / 遺伝子多型 / AS3MT / 代謝能力 / 感受性 / 途上国 / 民族差

Outline of Final Research Achievements

This study revealed following things; 1) under this experimental condition (drinking exposure with 100μg As/L for six months), relationship between arsenic exposure, metabolism, and toxicity was not clear in mice. 2) High concentrations of arsenic, manganese, and uranium were detected in groundwater from several areas in Myanmar, suggesting multi-elemental contamination and its human health risk. 3) AS3MT genetic polymorphism in Filipino and Burmese showed high frequency of the genotype with high metabolic capacity of arsenic.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、ヒ素のリスクに関する知見が深化・拡大されるとともに、科学的根拠に基づいた新たなリスク評価の指針を提供することができる。例えば、本研究によって得られた遺伝子情報から、ヒ素に対する感受性に合わせた飲料水のヒ素濃度の基準値を定めることができる。また、そうした知見は、とくに途上国において優先的にヒ素汚染の除去対策に取り組むべき地域の選定にも役立つであろう。
途上国現地ではヒ素の専門家が少なく、分析機器・試薬・技術も不十分なことから、現地政府・住民から海外の研究者への調査の要望も多い。本成果は途上国の公衆衛生・環境改善にも有益であり、国際社会的にも大きな貢献をもたらすものである。

Research Products

• [Int'l Joint Research] University of Rajshahi(バングラデシュ)
• [Int'l Joint Research] Mandalay Technological University(ミャンマー)
• [Int'l Joint Research] University of the Philippines(フィリピン)
• [Int'l Joint Research] CSIR Water Research Institute(ガーナ)
• [Int'l Joint Research] Patein University(ミャンマー)
• [Journal Article] E-waste recycling in Africa: risks and opportunities (2019)
  • Author(s): Asante Kwadwo Ansong、Amoyaw-Osei Yaw、Agusa Tetsuro
  • Journal Title: Current Opinion in Green and Sustainable Chemistry Volume: 18 Pages: 109-117
  • DOI: 10.1016/j.cogsc.2019.04.001
  • Peer Reviewed
• [Journal Article] Effects of 4-Hydroxy-2,3,3′,4′,5-Pentachlorobiphenyl (4-OH-CB107) on Liver Transcriptome in Rats: Implication in the Disruption of Circadian Rhythm and Fatty Acid Metabolism (2018)
  • Author(s): Ochiai, M., Iida, M., Agusa, T., Takaguchi, K., Fujii, S., Nomiyama, K., Iwata, H.
  • Journal Title: Toxicological Sciences Volume: 165 Issue: 1 Pages: 118-130
  • DOI: 10.1093/toxsci/kfy123
  • Peer Reviewed
• [Journal Article] Soil Pollution by Chlorobenzenes and Polychlorinated Biphenyls from an Electronic Waste Recycling Area in Northern Vietnam (2018)
  • Author(s): Nishimura, C., Suzuki, G., Matsukami, H., Agusa, T., Takaoka, M., Takahashi, S., Tue, N. M., Viet, P. H., Tanabe, S., Takigami, H., Fujimori, T.
  • Journal Title: International Journal of Environment and Pollution Volume: 63 Issue: 4 Pages: 283-297
  • DOI: 10.1504/ijep.2018.097863
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of lead speciation on its oral bioaccessibility in surface dust and soil of electronic-wastes recycling sites (2018)
  • Author(s): Fujimori.T, Taniguchi.M, Agusa.T, Shiota.K, Takaoka.M, Yoshida.A, Terazono.A, Ballesteros Jr, F.C.Takigami.H
  • Journal Title: Journal of Hazardous Materials Volume: 341 Pages: 363-372
  • DOI: 10.1016/j.jhazmat.2017.07.066
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Strain differences in the proteome of dioxin-sensitive and -resistant mice treated with 2,3,7,8-tetrabromodibenzo-p-dioxin (2017)
  • Author(s): Nguyen, H.T., Lauan Tsuchiya, M.C., Yoo, J., Iida, M., Agusa, T., Hirano, M., Kim, E.-Y., Miyazaki, T., Nose, M., Iwata, H.
  • Journal Title: Archives of Toxicology Volume: 4 Issue: 4 Pages: 1763-1782
  • DOI: 10.1007/s00204-016-1834-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hair mercury levels in relation to fish consumption among Vietnamese in Hanoi (2017)
  • Author(s): Hoang.V.A.T, Do.H.T.T, Agusa.T, Koriyama.C, Akiba.S, Ishibashi.Y, Sakamoto.M, Yamamoto.M
  • Journal Title: The Journal of Toxicological Sciences Volume: 42 Issue: 5 Pages: 651-662
  • DOI: 10.2131/jts.42.651
  • NAID: 130006076694
  • ISSN: 0388-1350, 1880-3989
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Lead contamination in surface soil on roads from used lead-acid battery recycling in Northern Vietnam (2016)
  • Author(s): Fujimori, T., Eguchi, A., Agusa, T., Tue, N. M., Suzuki, G., Takahashi, S., Viet, P. H., Tanabe, S. and Takigami, H.
  • Journal Title: Journal of Material Cycles and Waste Management Volume: 18(4) Issue: 4 Pages: 599-607
  • DOI: 10.1007/s10163-016-0527-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of aryl hydrocarbon receptor signaling pathways altered in TCDD- treated red seabream embryos by transcriptome analysis (2016)
  • Author(s): Midori Iida, Satoshi Fujii, Masaya Uchida, , Hiroshi Nakamura, Yoshihiro Kagami, Tetsuro Agusa, Masashi Hirano, Su-Min Bak, Eun-Young Kim, Hisato Iwata
  • Journal Title: Aquatic Toxicology Volume: 177 Pages: 156-170
  • DOI: 10.1016/j.aquatox.2016.05.014
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Contamination by Arsenic and Other Metals in Groundwater from Myanmar (2018)
  • Author(s): Agusa, T., Murakami, R., Kimura, N., Uchiyama, Y., Omagari, R., Ueda, H., Yoshida, M., Kawasaki, C., Arizono, K., Phay, N., Nakata, H.
  • Organizer: 5th International Arsenic Symposium in Miyazaki ~ Environmental Impact and Health Hazards ~
  • Int'l Joint Research
• [Presentation] Transboundary Pollution by Heavy Metals in East Asia and Its effect on Cetaceans (2018)
  • Author(s): Oda, A., Tsugeki, N.K., Kuwae, M., Tanabe, S., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management, Taoyuan
  • Int'l Joint Research
• [Presentation] Genotype Distribution of Arsenic Metabolic Enzymes in Asian Population. (2018)
  • Author(s): Fukuhara, K., Yoshida, A., Ballesteros, F.C., Hayama, S., Takuma, K., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management, Taoyuan
  • Int'l Joint Research
• [Presentation] Environmental Effects by Disaster Waste Caused by the Kumamoto Earthquake (2018)
  • Author(s): Kohara, M., Oda, A., Shimoda, Y., Nakata, H., Ueno, D., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management, Taoyuan
  • Int'l Joint Research
• [Presentation] Microplastic Pollution Originated from Drifted Wastes of Coastal Areas in Kumamoto (2018)
  • Author(s): Shimoda, Y., Oda, A., Matsumoto, Y., Kitahara, K., Nakata, H., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management, Taoyuan
  • Int'l Joint Research
• [Presentation] Environmental Contamination by Metals in Myanmar: Case Study of Arsenic in Groundwater (2018)
  • Author(s): Agusa, T., Murakami, R., Kimura, N., Omagari, R., Uchiyama, Y., Arizono, K., Nakata, H., Phay, N.
  • Organizer: Joint Seminar on Environmental Chemistry for Collaboration Research between Myanmar and Japan
  • Int'l Joint Research / Invited
• [Presentation] ミャンマーにおける地下水微量元素汚染の実態 (2018)
  • Author(s): 阿草哲郎, 村上理佳子, 木村直登, 大曲遼, 内山幸子, 有薗幸司, 中田晴彦, Phay Nyunt
  • Organizer: 第27回環境化学討論会
• [Presentation] Arsenic Pollution in Southeast Asia (2017)
  • Author(s): Agusa, T., Nakata, H., Iwata, H., Tanabe, S.
  • Organizer: Japan - Taiwan International Symposium on Environmental Science, Technology and Management
  • Place of Presentation: 北九州
  • Year and Date: 2017-03-30
  • Int'l Joint Research / Invited
• [Presentation] Contamination by Arsenic and Other Trace Elements in Groundwater from Myanmar (2017)
  • Author(s): Agusa, T., Murakami, R., Kimura, N., Uchiyama, Y., Omagari, R., Ueda, H., Yoshida, M., Kawasaki, C., Arizono, K., Phay, N., Nakata, H.
  • Organizer: 2017 Eastern Asia Conference for Water Environmental Issue
  • Int'l Joint Research / Invited
• [Presentation] Assessment of Ecological Impacts in Shirakawa River Damaged from a Landslide Caused by the Kumamoto Earthquake (2017)
  • Author(s): Aikoh, Y., Kimura, N., Murakami, R., Ishii, E., Hashimoto, M., Uchiyama, Y., Hosono, T., Nakata, H., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management
  • Int'l Joint Research
• [Presentation] Transboundary Pollution by Mercury and Identification of Its Pollution Sources in East Asia (2017)
  • Author(s): Sakamoto, A., Tsugeki, N.K., Kuwae, M., Inoue J., Nakata, H., Uchiyama, Y., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management
  • Int'l Joint Research
• [Presentation] Assessment of Toxicity of Chronic Arsenic Exposure Using Mice and Its Application to Humans (2017)
  • Author(s): Shimazu, I., Kawahara, M., Uchiyama, Y., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management
  • Int'l Joint Research
• [Presentation] Why Does Sweetfish (Plecoglossus altivelis) Accumulate Mercury? (2017)
  • Author(s): Takuma, K., Uchiyama, Y., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science, Technology and Management
  • Int'l Joint Research
• [Presentation] 2016年熊本地震による地下水質への影響：微量元素編 (2017)
  • Author(s): 阿草哲郎, 木村直登, 石井絵理, 橋本美穂, 村上理佳子, 内山幸子, 有薗幸司, 細野高啓, 中田晴彦
  • Organizer: 第26回環境化学討論会
• [Presentation] ミャンマーにおける地下水ヒ素汚染の実態 (2017)
  • Author(s): 阿草哲郎, 村上理佳子, 木村直登, 大曲遼, 内山幸子, 中田晴彦, Nyunt Phay
  • Organizer: 第23回ヒ素シンポジウム
• [Presentation] Water pollution by trace elements in Myanmar (2016)
  • Author(s): Murakami, R., Omagari, R., Uchiyama, Y., Nakata, H., Phay, N., Agusa, T.
  • Organizer: Third Myanmar-Japan International Symposium
  • Place of Presentation: Pathein, Myanmar
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Trace elements in groundwater after The 2016 Kumamoto Earthquakes (2016)
  • Author(s): Kimura, N., Murakami, R., Ishii, E., Hashioto, M., Uchiyama, Y., Hosono, T., Nakata, H., Agusa, T.
  • Organizer: Third Myanmar-Japan International Symposium
  • Place of Presentation: Pathein, Myanmar
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] メタボローム解析を用いたベトナムの地下水ヒ素汚染地域住民の影響評価：第2報 (2016)
  • Author(s): 阿草哲郎, 江口哲史, 國頭 恭, Tue, N.M., Lan, V.T.M., Minh, T.B., Trang, P.T.K., 高橋 真, Viet, P.H., 田辺信介, 岩田久人
  • Organizer: 第22回ヒ素シンポジウム
  • Place of Presentation: 江東区
  • Year and Date: 2016-11-17
• [Presentation] Contamination by trace elements associated with e-waste recycling processes in developing countries (2016)
  • Author(s): Agusa, T., Takigami, H., Fujimori, T., Eguchi, A., Bekki, K., Yoshida, A., Terazono, A., Ha, N.N., Asante, K.A., Biney, C.A., Agyekum, W.A., Bello, M., Ramu, K., Ballesteros Jr., F.C., Bulbule, K.A., Parthasaraty, P., Takahashi, S., Subramanian, A., Iwata, H., Tanabe, S.
  • Organizer: 2016 1st Eastern Asia Conference for Sustainable Economy (EACSE)
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Mercury pollution at small-scale gold mining and e-waste recycling areas in Indonesia (2016)
  • Author(s): Hayama, S., Uchiyama, Y., Sudaryanto, A., Yoshida, A., Oguchi, M., Terazono, A., Skandarl, Tanabe, A., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Urgent survey on groundwater pollution caused by The 2016 Kumamoto Earthquakes (2016)
  • Author(s): Kimura, N., Murakami, R., Ishii, E., Hashioto, M., Uchiyama, Y., Hosono, T., Nakata, H., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Chemical pollution in water environment in Myanmar (2016)
  • Author(s): Murakami, R., Omagari, R., Uchiyama, Y., Nakata, H., Phay, N., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] ベトナムの地下水ヒ素汚染とヒトへの曝露 (2016)
  • Author(s): 阿草哲郎
  • Organizer: 環境毒性化学とメダカに関する研究会 around 九州
  • Place of Presentation: 熊本
  • Year and Date: 2016-09-12
• [Presentation] メタボロミクスを用いたヒ素汚染地域住民の影響評価 (2016)
  • Author(s): 阿草哲郎, 江口哲史, Tue, N.M., Lan, V.T.M., Minh, T.B., Trang, P.T.K., 高橋 真, Viet, P.H., 田辺信介, 岩田久人
  • Organizer: 第25回環境化学討論会
  • Place of Presentation: 新潟
  • Year and Date: 2016-06-08
• [Book] Arsenic Contamination in Asia (2018)
  • Author(s): Kobayashi, Y., Agusa, T.
  • Total Pages: 211
  • Publisher: Springer Nature