| Budget Amount *help |
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
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| Outline of Final Research Achievements |
3-in-1 chip mimicking in vivo microenvironment was developed to simultaneously control oxygen tension and mechanical and chemical stimuli to cells. The microfluidic chip enabled to generate a uniform oxygen state down to 0.3% or a linear oxygen gradient within 15 minutes by supplying gas mixtures into the gas channels in the chip. Behavior of the human breast cancer cells suspended in type I collagen gel were then observed while controlling oxygen tension using the developed chip. As the results, it was revealed that the hypoxic environment promoted the proliferation and migration of the cancer cells. Moreover, the hypoxic response of breast cancer cells was changed by cell-cell interaction when co-cultured with vascular endothelial cells.
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