Fabrication of human liver with mimicking hepatic lobule structures by alternately-arranged human hepatocyte/endotherial cell tissue transplantation
| Project/Area Number |
16H05908
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
SAKAI Yusuke 長崎大学, 医歯薬学総合研究科(医学系), 助教 (10608904)
|
|---|
| Research Collaborator |
EGUCHI Susumu
NAKAZAWA Kohji
KOIKE Makiko
YAMANOUCHI Kosho
SOYAMA Akihiko
HIDAKA Masaaki
TAKATSUKI Mitsuhisa
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2016: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
|
|---|
| Keywords | 肝細胞 / 内皮細胞 / 肝臓 / アレイ化 / 再生医療 / 管腔 / 細胞シート / 血管新生 |
|---|
| Outline of Final Research Achievements |
Alternately-arranged human hepatocyte/ endothelial cell tissue (AHET) could contribute to oxygen supply, survival, three-dimensional organization, and maintenance of liver-specific functions. In this study, we aimed to fabricate AHET by using micropatterning technology and cell sheet engineering.
Multi-pored silicone film (MPSF) were prepared using microchannels. Human primary hepatocytes adhered to the perforated region of MPSF. The hepatocytes densely aggregated and were patterned. The hepatocytes were cocultured with HUVEC, and alternately-arranged human hepatocyte/ endothelial cell was generated. hADSC supported the formation of the luminal-like structure of HUVEC. AHET could be harvested and was an implantable tissue.
|
| Academic Significance and Societal Importance of the Research Achievements |
これまでに、規則的な血管網を持つ細胞だけで構成された移植用組織体の作製は困難であった。本研究では、肝小葉構造に基づき規則的に内皮細胞を配置すると共に、細胞のみで管腔構造を形成させる技術を確立した。ヒト初代肝細胞－内皮細胞索状組織（AHET）は、移植用の組織体として新しい移植医療に応用し得る可能性を秘めている。さらに、本技術を基盤として、他の機能性細胞（膵島細胞等）への迅速な応用が考え得る。これらのことから、次世代の移植再生医療技術として波及効果は多大である。
|
Report
(4 results)
Research Products
(29 results)
-
-
-
-
-
-
-
-
[Journal Article] Toyocamycin attenuates free fatty acid-induced hepatic steatosis and apoptosis in cultured hepatocytes and ameliorates nonalcoholic fatty liver disease in mice2017
Author(s)
Ikuko Takahara, Yuko Akazawa, Maiko Tabuchi, Katsuya Matsuda, Hisamitsu Miyaaki, Youko Kido, Yasuko Kanda, Naota Taura, Ken Ohnita, Fuminao Takeshima, Yusuke Sakai, Susumu Egushi, Masahiro Nakashima, Kazuhiko Nakao
-
Journal Title
PLoS One
Volume: 12
Issue: 3
Pages: e0170591-e0170591
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Up-regulated extracellular matrix components and inflammatory chemokines may impair the regeneration of cholestatic liver2016
Author(s)
Shuai Zhang, Tao-Sheng Li, Akihiko Soyama, Takayuki Tanaka, Chen Yan, Yusuke Sakai, Masaaki Hidaka, Ayaka Kinoshita, Koji Natsuda, Mio Fujii, Tota Kugiyama, Zhassulan Baimakhanov, Tamotsu Kuroki, Weili Gu, Susumu Eguchi
-
Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 26540-26540
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
