| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2016: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Outline of Final Research Achievements |
Alternately-arranged human hepatocyte/ endothelial cell tissue (AHET) could contribute to oxygen supply, survival, three-dimensional organization, and maintenance of liver-specific functions. In this study, we aimed to fabricate AHET by using micropatterning technology and cell sheet engineering.
Multi-pored silicone film (MPSF) were prepared using microchannels. Human primary hepatocytes adhered to the perforated region of MPSF. The hepatocytes densely aggregated and were patterned. The hepatocytes were cocultured with HUVEC, and alternately-arranged human hepatocyte/ endothelial cell was generated. hADSC supported the formation of the luminal-like structure of HUVEC. AHET could be harvested and was an implantable tissue.
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