| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2017: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
|---|
| Outline of Final Research Achievements |
Skeletal muscle physiology and the mechanisms of muscle diseases can be effectively studied by an in-vitro tissue model produced by tissue engineering. In this study, a human skeletal muscle tissue was produced by our newly developed method. To mimic native muscles, we focused on mechanical stresses. This study demonstrated that periodic exercise induced by continuous electrical stimulation enhanced the contractile ability of the engineered tissue. On the other hand, muscle contraction is controlled by nerve system in the body. From this viewpoint, we also attempted to produce a neuron-muscle tissue construct. Human iPS cells-derived motor neurons were seeded onto the tissue construct, and then the resultant tissue showed potential that human neurons physiologically connected to the engineered muscles. This new tissue engineering method could lead to truly biomimetic muscle tissue generation, and development of in-vitro physiological tissue models.
|
