Creation of elastic gel platform through the rational design of self-assembling polypeptides

• Project/Area Number: 16H05911
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Nagoya University
• Principal Investigator: Sugawara-Narutaki Ayae 名古屋大学, 工学研究科, 准教授 (10508203)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000); Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2016: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: ナノバイオ / 生体材料 / ポリペプチド / エラスチン / ゲル

Outline of Final Research Achievements

This study aimed to create new biomaterials as a platform for life science research through the development of easy-to-use elastin materials. The elastin-like block polypeptide GPGs developed by us are found to exhibit following characteristics. 1) GPGs form hydrogels at a concentration of two orders of magnitude lower than that of those existing elastin-based materials. 2) The fusion of a cell-adhesive sequence to GPG results in a nanofibers showing cell adhesive and proliferation properties equivalent to fibronectin toward fibroblast cells. 3) GPG nanofibers without the cell adhesion sequence are excellent in low platelet adhesion property.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、エラスチン類似ブロックポリペプチドGPGは、ハンドリング性、堅牢な自己集合性による分子設計の自由度の高さ、ファイバー表面への機能性モチーフ提示による高い生理活性等に優れる材料であることが示された。GPG類は、細胞のメカノトランスダクション研究や、細胞プリンティング用基材、人工血管や人工皮膚の創製など、ライフサイエンス研究のための新しいプラットフォームとして幅広い活用が期待される。

Research Products

• [Journal Article] Elastin-Like Polypeptides as Building Motifs toward Designing Functional Nanobiomaterials (2019)
  • Author(s): Duc H. T. Le, Ayae Sugawara-Narutaki
  • Journal Title: Molecular Systems Design & Engineering Volume: 印刷中 Issue: 3 Pages: 545-565
  • DOI: 10.1039/c9me00002j
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] エラスチンの可能性を拓く ～自己組織化とバイオマテリアル～ (2019)
  • Author(s): 鳴瀧彩絵
  • Journal Title: 生命化学研究レター Volume: 58 Pages: 11-16
  • Open Access
• [Journal Article] 自己集合性ブロックポリペプチドによる機能性ナノファイバーの創製 (2018)
  • Author(s): 鳴瀧彩絵
  • Journal Title: Peptide Newsletter Japan Volume: 110 Pages: 9-12
  • Open Access
• [Journal Article] Double-Hydrophobic Elastin-Like Polypeptides with Added Functional Motifs: Self-Assembly and Cytocompatibility (2017)
  • Author(s): Duc H. T. Le, Yoko Tsutsui, Ayae Sugawara-Narutaki, Hiroshi Yukawa, Yoshinobu Baba, Chikara Ohtsuki
  • Journal Title: Journal of Biomedical Materials Research Part A Volume: 105 Issue: 9 Pages: 2475-2484
  • DOI: 10.1002/jbm.a.36105
  • Peer Reviewed
• [Journal Article] 目の下のシワを消すローション!? －第二の皮膚として機能する生体模倣ポリマー (2016)
  • Author(s): 鳴瀧彩絵
  • Journal Title: 化学 Volume: 71(10) Pages: 39-42
• [Presentation] ブロック高分子を活用したナノバイオ材料の構築 (2019)
  • Author(s): 鳴瀧彩絵
  • Organizer: 第一回光量子バイオ分析診断技術研究会
• [Presentation] Self-Assembled Bio-Nanomaterials Designed by Block Copolymers (2018)
  • Author(s): Ayae Sugawara-Narutaki
  • Organizer: JSPS-CNRS Progress Meeting
  • Int'l Joint Research / Invited
• [Presentation] Cell Behavior on Self-Assembled Polypeptide Nanofibers (2018)
  • Author(s): 鳴瀧彩絵
  • Organizer: ナノ学会 ナノ構造・物性－ナノ機能・応用部会合同シンポジウム
• [Presentation] エラスチン類似ポリペプチドの配列デザインによる機能性ナノファイバーの構築 (2018)
  • Author(s): 鳴瀧彩絵
  • Organizer: 第49回 中部化学関係学協会支部連合秋季大会
• [Presentation] ブロック高分子が拓くナノマテリアルデザイン (2018)
  • Author(s): 鳴瀧彩絵
  • Organizer: 高分子学会九州支部フォーラム
  • Invited
• [Presentation] Nanofibrous Scaffolds from Elastin-Like Block Polypeptides (2018)
  • Author(s): Ayae Sugawara-Natutaki, Shunsuke Nara, Jin Nakamura, Chikara Ohtsuki
  • Organizer: 5th TERMIS World Congress 2018
  • Int'l Joint Research
• [Presentation] Thermoresponsive Hydrogels from Double-Hydrophobic Elastin-Like Polypeptides (2018)
  • Author(s): Ayae Sugawara-Narutaki, Sawako Yasunaga, Chikara Ohtsuki
  • Organizer: Biomaterials International 2018
  • Int'l Joint Research
• [Presentation] Biofunctional Nanofibers of Self-Assembled Elastin-Like Polypeptides (2018)
  • Author(s): Ayae Sugawara-Narutaki
  • Organizer: MIRAI Materials Science Workshop 2018 Spring
  • Int'l Joint Research
• [Presentation] Cell Behavior on Self-Assembled Nanofibers of Elastin-Like Polypeptides (2018)
  • Author(s): Kazuki Natsume, Ayae Sugawara-Narutaki, Jin Nakamura, Kazuhide Sato, Chikara Ohtsuki
  • Organizer: 1st G'L'owing Polymer Symposium in KANTO
  • Int'l Joint Research
• [Presentation] Effect of Block Length on the Self-Assembly of Elastin-Like Block Polypeptides (2017)
  • Author(s): Ayae Sugawara-Narutaki, Sawako Yasunaga, Duc H. T. Le, Chikara Ohtsuki
  • Organizer: 日本化学会第97春季年会（2017）
  • Place of Presentation: 慶應義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] Double-Hydrophobic Elastin-Like Polypeptides Assembling into Beaded Nanofibers (2017)
  • Author(s): Ayae Sugawara-Narutaki
  • Organizer: 第17回日本蛋白質科学会年会
  • Invited
• [Presentation] Effect of Block Length on the Self-Assembly and Gelation Behavior of Elastin-Like Polypeptides (2017)
  • Author(s): Sawako Yasunaga, Ayae Sugawara-Narutaki, Duc H. T. Le, Chikara Ohtsuki
  • Organizer: 第 9 回名大－清華大－トヨタ－北大合同シンポジウム , 2017年 , ポスター（一般）
  • Int'l Joint Research
• [Presentation] Design and Construction of Elastin-Like Block Polypeptides Assembling into Beaded Nanofibers (2017)
  • Author(s): Ayae Sugawara-Narutaki, Sawako Yasunaga, Duc H. T. Le, Chikara Ohtsuki
  • Organizer: IUMRS-ICAM2017 (The 15th International Conference on Advanced Materials)
  • Int'l Joint Research
• [Presentation] Development of Functional Nanofibers from Elastin-Like Block Polypeptides (2017)
  • Author(s): Ayae Sugawara-Narutaki, Sawako Yasunaga, Duc H. T. Le, Chikara Ohtsuki
  • Organizer: 2nd Global Congress & Expo on Materials Science & Nanoscience
  • Int'l Joint Research
• [Presentation] エラスチン類似ポリペプチドの配列設計による自己集合性の制御とハイドロゲルの形成 (2017)
  • Author(s): 鳴瀧彩絵，安永佐和子，奈良俊佑，中嶋優友，Duc H. T. Le，中村仁，大槻主税
  • Organizer: 第39回日本バイオマテリアル学会大会
• [Presentation] Design, Construction and Functionalization of Elastin-Like Block Polypeptides Assembling into Beaded Nanofibers (2017)
  • Author(s): 鳴瀧彩絵
  • Organizer: ナノ学会 ナノ構造・物性－ナノ機能・応用部会合同シンポジウム
  • Invited
• [Presentation] ブロック高分子が拓く自己組織化とバイオ応用 (2016)
  • Author(s): 鳴瀧彩絵
  • Organizer: 新領域研究グループ「エキゾチック自己組織化材料」「金属と分子集合」 第１回合同シンポジウム
  • Place of Presentation: 温泉旅館 丸茂 会議室（鳥取県鳥取市）
  • Year and Date: 2016-11-23
• [Presentation] ナノファイバー形成能を持つエラスチン類似ポリペプチドへの配列付加による機能化 (2016)
  • Author(s): 鳴瀧彩絵, Duc H. T. Le, 安永佐和子, 筒井陽子, 湯川博, 馬場嘉信, 大槻主税
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-11-21
• [Presentation] Elastin-Like Block Polypeptides Assembling into Beaded Nanofibers (2016)
  • Author(s): Ayae Sugawara-Narutaki, Duc H. T. Le, Noriki Kubota, Chikara Ohtsuki
  • Organizer: The 3rd International Conference on Bioinspired and Biobased Chemistry & Materials (N.I.C.E. 2016)
  • Place of Presentation: Nice, France
  • Year and Date: 2016-10-16
  • Int'l Joint Research
• [Presentation] エラスチン類似ブロックポリペプチドによる機能性ナノファイバーの構築 (2016)
  • Author(s): 鳴瀧彩絵, Duc H. T. Le, 久保田紀城, 筒井陽子, 湯川博, 馬場嘉信, 大槻主税
  • Organizer: 第65回高分子討論会
  • Place of Presentation: 神奈川大学横浜キャンパス（神奈川県横浜市）
  • Year and Date: 2016-09-14
• [Book] 医療・診断をささえるペプチド科学 ー再生医療・DDS・診断への応用ー (2017)
  • Author(s): 鳴瀧彩絵, 大槻主税（分担執筆）
  • Total Pages: 315
  • Publisher: シーエムシー出版
  • ISBN: 9784781312675
• [Remarks] 名古屋大学教員データベースシステム
  • URL: http://profs.provost.nagoya-u.ac.jp/view/html/100007263_ja.html
• [Remarks] 名古屋大学 教員詳細
  • URL: http://profs.provost.nagoya-u.ac.jp/view/html/100007263_ja.html