| Budget Amount *help |
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2016: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
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| Outline of Final Research Achievements |
We have been studying that the relationship between aging and environmental transcriptional factor, Nrf1, using genetical modified mouse and zebrafish. Especially, we have found that genetical aging model mice, Klotho-knockout mice with modulate the Nrf1 expression profile could live longer compared with single klotho knockout mice. Consequently, Klotho-/- :: Nrf1-knockdown mice were lived 20 weeks after birth. From those experiment we have identified 9 aging surrogate genetical marker which can explain the aging stage. We have tried to generate overexpress and/or knockout zebrafish model for those genes. As a consequence, we have already identified that serpina 4 and ccl4 knockout fish showed early aging phenotype. In addition, from metabolomic analysis compared with wild type, klotho knockout mouse and natural aging mice (over 3-years-old), we have identified that polyamine pathway have a several contribution to prevention of the aging phenotype.
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