Analysis of cell surface glycan networks to elucidate the function of glycan posttranslatinal modification on membrane proteins

• Project/Area Number: 16H05924
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomolecular chemistry
• Research Institution: Osaka University
• Principal Investigator: Manabe Yoshiyuki 大阪大学, 理学研究科, 助教 (00632093)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000); Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: 糖鎖合成 / 糖鎖修飾 / 免疫 / ケミカルバイオロジー / N-グリカン / 糖鎖 / 複合化 / レクチン / 糖鎖機能解明 / イメージング / HaloTag / コアフコース

Outline of Final Research Achievements

Asparagine-linked oligosaccharides (N-glycans) on glycoproteins have high diversity and complexity and are involved in a variety of important physiological events. We achieved the synthesis of several complex type N-glycans, including core fucos containing N-glycan, bisecting GlcNAc containing N-glycan, and poly lactosamine containing N-glycans. We carried out live cell imaging using HaloTag to elucidate the function of N-glycans on membrane proteins. We prepared HaloTag-expressing cells and presented synthetic glycans. Then, we succesfully demonstrated that the interaction between glycans and lectins can regulate the dynamics of membrane proteins. Furthermore, we discovered the lectin recognizing core fucose. In addition, we also developed effective cancer vaccines with focusing on the relationship between glycan and immunity.

Academic Significance and Societal Importance of the Research Achievements

一部の可溶性タンパク質の修飾糖鎖に関しては，その機能発現機構が解明されつつあるが，膜タンパク質については，その糖鎖構造に基づく機能解析は進んでいない．本研究では，N-グリカンの化学合成を達成し，Tagテクノロジーにより，厳密に糖鎖構造を制御したモデル膜タンパク質を調製し，膜タンパク質における糖鎖の機能の一端を明らかにし，膜タンパク質における糖鎖機能解明の大きな一歩である．N-グリカンによる膜タンパク質の機能制御が，がんなどのさまざまな疾病と関連することから，糖鎖機能の制御という新しい概念に基づいた創薬につながる可能性もある．また，糖鎖機能解明の新しい方法論を提案したという点でも意義が大きい．

Research Products

• [Int'l Joint Research] Academia Sinica/精華大学(中国)
• [Int'l Joint Research] University of Napoli Federico II(イタリア)
• [Int'l Joint Research] University of Napoli Federico II(イタリア)
• [Int'l Joint Research] Academia Sinica(台湾)
• [Int'l Joint Research] 精華大学(台湾)
• [Journal Article] α‐Emitting cancer therapy using 211 At‐AAMT targeting LAT1 (2021)
  • Author(s): Kaneda‐Nakashima Kazuko、Zhang ZiJian、Manabe Yoshiyuki、Shimoyama Atsushi、Kabayama Kazuya、Watabe Tadashi、Kanai Yoshikatsu、Ooe Kazuhiro、Toyoshima Atsushi、Shirakami Yoshifumi、Yoshimura Takashi、Fukuda Mitsuhiro、Hatazawa Jun、Nakano Takashi、Fukase Koichi、Shinohara Atsushi
  • Journal Title: Cancer Science Volume: 112 Issue: 3 Pages: 1132-1140
  • DOI: 10.1111/cas.14761
  • Peer Reviewed / Open Access
• [Journal Article] Recent Advances in the Chemical Biology of N-Glycans (2021)
  • Author(s): Shirakawa Asuka、Manabe Yoshiyuki、Fukase Koichi
  • Journal Title: Molecules Volume: 26 Issue: 4 Pages: 1040-1040
  • DOI: 10.3390/molecules26041040
  • Peer Reviewed / Open Access
• [Journal Article] Behavior of glycolylated sialoglycans in the binding pockets of murine and human CD22 (2021)
  • Author(s): Di Carluccio Cristina、Forgione Rosa Ester、Montefiori Marco、Civera Monica、Sattin Sara、Smaldone Giovanni、Fukase K.、Manabe Y.、Crocker Paul R.、Molinaro Antonio、Marchetti Roberta、Silipo Alba
  • Journal Title: iScience Volume: 24 Issue: 1 Pages: 101998-101998
  • DOI: 10.1016/j.isci.2020.101998
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Synthesis of ABO blood group antigens and functional glycan display on the cell surface (2020)
  • Author(s): Tsutsui Masato、Manabe Yoshiyuki、Kabayama Kazuya、Fukase Koichi
  • Journal Title: Arkivoc Volume: 2021 Issue: 4 Pages: 168-185
  • DOI: 10.24820/ark.5550190.p011.362
  • Peer Reviewed / Open Access
• [Journal Article] Recent advances in self-adjuvanting glycoconjugate vaccines (2020)
  • Author(s): Manabe Yoshiyuki、Chang Tsung-Che、Fukase Koichi
  • Journal Title: Drug Discovery Today: Technologies Volume: 2020 Pages: 1-1
  • DOI: 10.1016/j.ddtec.2020.11.006
  • Peer Reviewed
• [Journal Article] Concise and Reliable Syntheses of Glycodendrimers via Self-Activating Click Chemistry: A Robust Strategy for Mimicking Multivalent Glycan?Pathogen Interactions (2020)
  • Author(s): Farabi K, Manabe Y, Ichikawa H, Miyake S, Tsutsui M, Kabayama K, Yamaji T, Tanaka K, Hung S-C, Fukase K.
  • Journal Title: J Org Chem Volume: 85 Issue: 24 Pages: 16014-16023
  • DOI: 10.1021/acs.joc.0c01547
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Total Syntheses of C60- and C100-Dolichols (2020)
  • Author(s): Hirao Kohtaro、Ono Risako、Manabe Yoshiyuki、Masui Seiji、Atomi Haruyuki、Fukase Koichi
  • Journal Title: The Journal of Organic Chemistry Volume: 85 Issue: 17 Pages: 11549-11559
  • DOI: 10.1021/acs.joc.0c01327
  • Peer Reviewed
• [Journal Article] Unveiling Molecular Recognition of Sialoglycans by Human Siglec-10 (2020)
  • Author(s): Forgione Rosa Ester、Di Carluccio Cristina、Guzman-Caldentey Juan、Gaglione Rosa、Battista Filomena、Chiodo Fabrizio、Manabe Yoshiyuki、Arciello Angela、Del Vecchio Pompea、Fukase Koichi、Molinaro Antonio、Martin-Santamaria Sonsoles、Crocker Paul R.、Marchetti Roberta、Silipo Alba
  • Journal Title: iScience Volume: 23 Issue: 6 Pages: 101231-101231
  • DOI: 10.1016/j.isci.2020.101231
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Middle Molecular and Conjugation Strategies for Development of Bioactive Middle Molecules (2020)
  • Author(s): Manabe Yoshiyuki、Shimoyama Atushi、Kabayama Kazuya、Fukase Koichi
  • Journal Title: Journal of Synthetic Organic Chemistry, Japan Volume: 78 Issue: 5 Pages: 527-537
  • DOI: 10.5059/yukigoseikyokaishi.78.527
  • NAID: 130007841845
  • Peer Reviewed
• [Journal Article] Immunological Evaluation of Co‐Assembling a Lipidated Peptide Antigen and Lipophilic Adjuvants: Self‐Adjuvanting Anti‐Breast‐Cancer Vaccine Candidates (2020)
  • Author(s): Aiga Taku、Manabe Yoshiyuki、Ito Keita、Chang Tsung‐Che、Kabayama Kazuya、Ohshima Shino、Kametani Yoshie、Miura Ayane、Furukawa Hiroto、Inaba Hiroshi、Matsuura Kazunori、Fukase Koichi
  • Journal Title: Angewandte Chemie International Edition Volume: 59 Issue: 40 Pages: 17705-17711
  • DOI: 10.1002/anie.202007999
  • Peer Reviewed
• [Journal Article] Development of α-Gal-Antibody Conjugates to Increase Immune Response by Recruiting Natural Antibodies (2019)
  • Author(s): Sianturi Julinton、Manabe Yoshiyuki、Li Hao-Sheng、Chiu Li-Ting、Chang Tsung-Che、Tokunaga Kento、Kabayama Kazuya、Tanemura Masahiro、Takamatsu Shinji、Miyoshi Eiji、Hung Shang-Cheng、Fukase Koichi
  • Journal Title: Angewandte Chemie International Edition Volume: 58 Issue: 14 Pages: 4526-4530
  • DOI: 10.1002/anie.201812914
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Kinetically Controlled Fischer Glycosidation under Flow Conditions: A New Method for Preparing Furanosides (2019)
  • Author(s): Fukase Koichi、Masui Seiji、Manabe Yoshiyuki、Hirao Kohtaro、Shimoyama Atsushi、Fukuyama Takahide、Ryu Ilhyong
  • Journal Title: Synlett Volume: 30 Issue: 04 Pages: 397-400
  • DOI: 10.1055/s-0037-1611643
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis And Immunological Evaluation of Self-adjuvanting Anticancer Vaccine Candidate. (2019)
  • Author(s): Manabe, Y., Chang, T-C., Feng, Q., Fujimoto, Y., Ohshima, S., Kametani, Y., Kabayama, K., Nimura, Y., Lin, C-C., Fukase, K.
  • Journal Title: Peptide Science Volume: 2019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Single-Step Per-O-Sulfonation of Sugar Oligomers with Concomitant 1,6-Anhydro Bridge Formation for Binding Fibroblast Growth Factors (2018)
  • Author(s): Yeh Che-Jui、Ku Chiao-Chu、Lin Wei-Chen、Fan Chiao-Yuan、Zulueta Medel Manuel L.、Manabe Yoshiyuki、Fukase Koichi、Li Yaw-Kuen、Hung Shang-Cheng
  • Journal Title: ChemBioChem Volume: 20 Issue: 2 Pages: 237-240
  • DOI: 10.1002/cbic.201800464
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Branched Sialylated <i>N</i>-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H (2018)
  • Author(s): M. Handa-Narumi, T. Yoshimura, H. Konishi, Y. Fukata, Y. Manabe, K. Tanaka, G.-m. Bao, H. Kiyama, K. Fukase, K. Ikenaka
  • Journal Title: Cell Structure and Function Volume: 43 Issue: 2 Pages: 141-152
  • DOI: 10.1247/csf.18009
  • NAID: 130007439439
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access
• [Journal Article] Convergent synthesis of a bisecting GlcNAc-containing N-glycan (2018)
  • Author(s): Manabe Yoshiyuki、Shomura Hiroki、Minamoto Naoya、Nagasaki Masahiro、Takakura Yohei、Tanaka Katsunori、Silipo Alba、Molinaro Antonio、Fukase Koichi
  • Journal Title: Chemistry - An Asian Journal Volume: 印刷中 Issue: 12 Pages: 1544-1551
  • DOI: 10.1002/asia.201800367
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Syntheses and Immunological Evaluation of Self-Adjuvanting Clustered N-Acetyl and N-Propionyl Sialyl-Tn Combined with a T-helper Cell Epitope as Antitumor Vaccine Candidates (2018)
  • Author(s): Chang Tsung-Che、Manabe Yoshiyuki、Fujimoto Yukari、Ohshima Shino、Kametani Yoshie、Kabayama Kazuya、Nimura Yuka、Lin Chun-Cheng、Fukase Koichi
  • Journal Title: Angewandte Chemie International Edition Volume: 57 Issue: 27 Pages: 8219-8224
  • DOI: 10.1002/anie.201804437
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Narrower HOMO-LUMO gap attained by conformational switching through peripheral polyarylation in 1,4,5,8-tetraaza-9,10-anthraquinodimethanes (2018)
  • Author(s): Suzuki Takanori、Ishigaki Yusuke、Sugawara Kazuma、Umezawa Yu、Katoono Ryo、Shimoyama Atsushi、Manabe Yoshiyuki、Fukase Koichi、Fukushima Takanori
  • Journal Title: Tetrahedron Volume: 74 Issue: 18 Pages: 2239-2244
  • DOI: 10.1016/j.tet.2018.03.041
  • Peer Reviewed
• [Journal Article] Synthesis and Functional Studies of Self-Adjuvanting Cancer Vaccine Containing B Cell Epitope Derived from HER2. (2017)
  • Author(s): Feng, Q., Kabayama, K., Manabe, Y., Miyamoto, A., Kametani, Y., Fukase, K.
  • Journal Title: Peptide Science Volume: 2017 Pages: 56-57
  • Peer Reviewed
• [Journal Article] Development of alpha1,6-fucosyltransferase inhibitors through the diversity-oriented syntheses of GDP-fucose mimics using the coupling between alkyne and sulfonyl azide. (2017)
  • Author(s): Manabe, Y., Kasahara, S., Takakura, Y., Yang, X., Takamatsu, S., Kamada Y., Miyoshi, M., Yoshidome, D., Fukasea, K.
  • Journal Title: Bioorg. Med. Chem. Volume: in press Issue: 11 Pages: 2844-2850
  • DOI: 10.1016/j.bmc.2017.02.036
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Flow Dehydration and Hydrogenation of Allylic Alcohols: Application to the Waste-Free Synthesis of Pristane (2017)
  • Author(s): Furuta, A.; Hirobe, Y.; Fukuyama, T.; Ryu, I.
  • Journal Title: Eur. J. Org. Chem. Volume: 2017 Issue: 10 Pages: 1365-1368
  • DOI: 10.1002/ejoc.201700072
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preparation of the Pseudo Membrane Glycoprotein Using Halo Tag Technology: Novel Approach for the Functional Analysis of the Glycan on Cell Surface. (2016)
  • Author(s): Miyake, S.; Manabe, Y.; Shomura, H.; Kabayama, K.; Yamaji, T.; Hanada, K.; Fukase, K.
  • Journal Title: Peptide Science Volume: 2016 Pages: 216-216
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of a Complex-Type N-Glycan Containing a Core Fucose. (2016)
  • Author(s): Nagasaki, M.; Manabe, Y.; Minamoto, N.; Tanaka, K.; Silipo, A.; Molinaro, A.; Fukase, K.
  • Journal Title: J. Org. Chem. Volume: 81 Issue: 22 Pages: 10600-10616
  • DOI: 10.1021/acs.joc.6b02106
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of a simple assay system for protein-stabilizing efficiency based on hemoglobin protection against denaturation and measurement of the cooperative effect of mixing protein stabilizers. (2016)
  • Author(s): Chen, S.; Manabe, Y.; Minamoto, Y.; Saiki, N.; Fukase, K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 80 Issue: 10 Pages: 1874-1878
  • DOI: 10.1080/09168451.2016.1189317
  • Peer Reviewed
• [Presentation] Synthetic Biology Approach for Elucidating the N-Glycan Functions (2021)
  • Author(s): Yoshiyuki Manabe
  • Organizer: 4th A3 Young Scientist Meeting
  • Int'l Joint Research / Invited
• [Presentation] Siglecとの相互作用解析のためのシアル酸含有N-グリカンの合成 (2021)
  • Author(s): 白川明日香、真鍋良幸、出村健太、増井誠二、深瀬浩一
  • Organizer: 第101回日本化学会春季年会
• [Presentation] カゴ型ホウ素錯体によるフッ化糖を用いたグリコシル化反応の開発 (2021)
  • Author(s): 生長優香、笹谷祥大、真鍋良幸、角永悠一郎、小西彬仁、安田誠、深瀬浩一
  • Organizer: 第101回日本化学会春季年会
• [Presentation] ドリコールの全合成および合成プローブを用いたドリコールの機能解析 (2021)
  • Author(s): 小野莉紗子，平尾宏太郎，真鍋良幸，梅川雄一，村田道雄，深瀬浩一
  • Organizer: 第101回日本化学会春季年会
• [Presentation] 共会合型セルフアジュバンティング乳がんワクチンの合成と免疫機能評価 (2021)
  • Author(s): 伊藤啓太，真鍋良幸，相賀拓，樺山一哉，大島志乃，亀谷美恵，古川寛人，稲葉央，松浦和則，深瀬浩一
  • Organizer: 第101回日本化学会春季年会
• [Presentation] Synthetic study of N-glycan from hyperthermophilic archaeon Thermococcus kodakarensis (2021)
  • Author(s): K. Hirao, Y. Manabe, I. Speciale, H. Atomi, A. Molinaro, C. De, Castro. K. Fukase
  • Organizer: 第101回日本化学会春季年会
• [Presentation] Analysis of Membrane Protein Dynamics by Glycan-galectin Interaction Using Synthetic Glycan-displaying Cells (2021)
  • Author(s): 三浦彩音 ・樺山一哉 ・真鍋良幸・三宅秀斗・白川明日香・初村洋紀・山地俊之・鈴木健一・深瀬浩一
  • Organizer: 東海大学マイクロ・ナノ啓発会
• [Presentation] Analysis of Membrane Protein Dynamics by Glycan-galectin Interaction Using Synthetic Glycan-displaying Cells (2020)
  • Author(s): Ayane Miura, Syuto Miyake, Kazuya Kabayama, Yoshiyuki Manabe, Asuka Shirakawa, Hiroki Syomura, Toshiyuki Yamaji, Kenichi Suzuki, Koichi Fukase
  • Organizer: Academia Sinica-Osaka University Symposium 2020
  • Int'l Joint Research
• [Presentation] Immunological Evaluation of Conjugate/Co-Assembly of Peptide Antigen and Adjuvant as Self-adjuvanting Anti-breast Cancer Candidates (2020)
  • Author(s): Keita Ito, Yoshiyuki Manabe, Taku Aiga, Tsung-Che Chang, Kazuya Kabayama, Shino Ohshima, Yoshie Kametani, Hiroto Furukawa, Hiroshi Inaba, Kazunori Matsuura, and Koichi Fukase
  • Organizer: 第57回ペプチド討論会
• [Presentation] Siglecとの相互作用解明を目指した重水素標識シアル酸含有N-グリカンの合成研究 (2020)
  • Author(s): 白川明日香、真鍋良幸、出村健太、増井誠二、深瀬浩一
  • Organizer: 第10回CSJ化学フェスタ
• [Presentation] コアフコースの機能制御を目指したFUT8阻害剤の開発 (2020)
  • Author(s): 柳原 茉由、高倉 陽平、真鍋 良幸、深瀬 浩一
  • Organizer: 第10回CSJ化学フェスタ
• [Presentation] コアフコース含有N-グリカンの機能解明・制御を目指したケミカルバイオロジー (2020)
  • Author(s): 真鍋 良幸，Roberta Marchetti，武部 智之，笠原 里実，高倉 陽平，二瓶 渉，田中 克典，樺山 一哉， Fabrizio Chiodo，花島 慎弥，鎌田 佳宏，三善 英知， Hari Prasad Dulal，山口 芳樹，安達 禎之，大野 尚仁，田中 浩士， Alba Silipo， Antonio Molinaro，深瀬 浩一
  • Organizer: 第62回天然有機化合物討論会
• [Presentation] Synthesis and function study of glycoconjugate vaccine for efficient cancer (2019)
  • Author(s): Yoshiyuki Manabe
  • Organizer: 日本化学会 第99春季年会 (2019)
  • Int'l Joint Research / Invited
• [Presentation] Synthesis and immunological evaluation of self-adjuvanting anticancer vaccine candidate (2018)
  • Author(s): Yoshiyuki Manabe, Tsung-Che Chang, Qi Feng, Yukari Fujimoto, Shino Ohshima, Yoshie Kametani, Kazuya Kabayama, Yuka Nimura, Chun-Cheng Lin, Koichi Fukase
  • Organizer: 10th International Peptide Symposium
  • Int'l Joint Research
• [Presentation] Syntheses of complex type N-glycans and live cell imaging of their protein conjugates (2018)
  • Author(s): Yoshiyuki Manabe, Masahiro Nagasaki, Hiroki Shomura, Naoya Minamoto, Soichiro Okamura, Shuto Miyake, Kanae Hatano, Kazuya Kabayama and Koichi Fukase
  • Organizer: the 14th International Kyoto Conference on New Aspects of Organic Chemistry (IKCOC-14)
  • Int'l Joint Research
• [Presentation] Efficient syntheses of ABO blood type antigens and its immune evaluation (2018)
  • Author(s): Masato Tsutsui, Yoshiyuki Manabe, Koichi Fukase
  • Organizer: the 14th International Kyoto Conference on New Aspects of Organic Chemistry (IKCOC-14)
  • Int'l Joint Research
• [Presentation] Synthesis and Anti-tumor Evaluation of At-211 Labeled Amino Acid Derivative for Targeted Alpha-particle Therapy (2018)
  • Author(s): Z. Zhang, K. Kaneda, A. Toyoshima, Y. Manabe, K. Kabayama, A. Shimoyama, A. Shinohara, K. Fukase
  • Organizer: the 14th International Kyoto Conference on New Aspects of Organic Chemistry (IKCOC-14)
  • Int'l Joint Research
• [Presentation] Chemical syntheses of ABO blood type antigens using diacetyl strategy and investigation of their immune response (2018)
  • Author(s): Masato Tsutsui, Yoshiyuki Manabe, Koichi Fukase
  • Organizer: The 3rd A3 Roundtable Meeting on Chemical Probe Research Hub
  • Int'l Joint Research
• [Presentation] Preparation of novel anticancer drugs using At-211 (2018)
  • Author(s): K. Kabayama, K. Kaneda-Nakashima, Y. Manabe, A. Shimoyama, A. Toyoshima, A. Shinohara, K. Fukase
  • Organizer: 5th Asian Chemical Biology Conference (ACBC-5)
  • Int'l Joint Research
• [Presentation] Reconstruction of glycan environments (2018)
  • Author(s): K. Fukase, Y. Manabe, K. Kabayama, H. Ichikawa, S. Miyake, H. Shomura, T. Yamaji, K. Hanada, S.-C. Hung
  • Organizer: 5th Asian Chemical Biology Conference (ACBC-5)
  • Int'l Joint Research
• [Presentation] SYNTHESIS OF COMPLEX TYPE N-GLYCANS (2018)
  • Author(s): Yoshiyuki Manabe, Masahiro Nagasaki, Hiroki Shomura, Naoya Minamoto, Soichiro Okamura and Koichi Fukase
  • Organizer: 29th International Carbohydrate Symposium (ICS 2018)
  • Int'l Joint Research
• [Presentation] ハイスループットスクリーニングによるα1,6-フコシルトランスフェラーゼ (FUT8) 阻害剤の探索と構造展開 (2018)
  • Author(s): 武部智之、真鍋良幸、笠原里美、Yang XiaoXiao、高松真二、三善英知、樺山一哉、深瀬浩一
  • Organizer: 第99回日本化学会年会
• [Presentation] 多分枝N-結合型糖鎖の網羅的合成を可能とする多様性指向型合成戦略の検討 (2018)
  • Author(s): 白川明日香、真鍋良幸、出村健太、増井誠二、深瀬浩一
  • Organizer: 第99回日本化学会年会
• [Presentation] 超好熱性アーキアのN-グリカン担体脂質ドリコールの合成研究 (2018)
  • Author(s): 平尾宏太郎、真鍋良幸、小野莉紗子、深瀬浩一
  • Organizer: 第99回日本化学会年会
• [Presentation] N-結合型糖鎖の効率合成とそれを用いた合成生物学的研究 (2018)
  • Author(s): 真鍋良幸
  • Organizer: 日本化学会新領域研究グループ「有機合成化学を起点とするものづくり戦略」最終研究成果発表シンポジウム
• [Presentation] Z選択的Wittig反応を鍵とするドリコールの合成研究 (2018)
  • Author(s): 平尾宏太郎、真鍋良幸、小野莉紗子、深瀬浩一
  • Organizer: 第8回CSJ化学フェスタ2018
• [Presentation] N-結合型糖鎖の効率合成とその機能解明を目指したライブセルイメージング (2018)
  • Author(s): 真鍋良幸、長崎政裕、初村洋紀、 源 直也、岡村壮一郎、三宅秀斗、波多野佳奈枝、樺山一哉、田中克典、深瀬浩一
  • Organizer: 第60回天然有機化合物討論会
• [Presentation] マイクロフロー系でのグリコシル化反応の開発：速度論支配のグリコシル化と不安定グリコシルドナー体の利用 (2018)
  • Author(s): 増井誠二、真鍋良幸、福山高英、柳 日馨、深瀬浩一
  • Organizer: 第7回 JACI/GSC シンポジウム
• [Presentation] マイクロフロー系を用いた α-gal エピトープの効率合成とがん免疫療法への適用 (2018)
  • Author(s): Julinton Sianturi、真鍋良幸、樺山一哉、深瀬浩一
  • Organizer: 第7回 JACI/GSC シンポジウム
• [Presentation] Efficient Glycosylations Using Micro Fluidic System (2018)
  • Author(s): Seiji Masui, Yoshiyuki Manabe, Kento Tokunaga, Atsushi Shimoyama, Takahide Fukuyama, Koichi Fukase.
  • Organizer: The First International Conference on Automated Flow and Microreactor Synthesis（ICAMS-1）
  • Int'l Joint Research
• [Presentation] Efficient Synthesis of α-Gal Using One-Pot and Microfluidic Approaches for Development of a Novel Cancer Immunotherapy (2018)
  • Author(s): Julinton Sianturi, Yoshiyuki Manabe, Kazuya Kabayama, Koichi Fukase
  • Organizer: The First International Conference on Automated Flow and Microreactor Synthesis（ICAMS-1）
  • Int'l Joint Research
• [Presentation] 膜タンパク質の合成糖鎖による修飾および生細胞における機能解析 (2018)
  • Author(s): 三宅秀斗、樺山一哉、初村洋紀、山地俊之、花田賢太郎、真鍋良幸、深瀬浩一
  • Organizer: 第98回日本化学年会
• [Presentation] アルキンとスルホンアジドのカップリング反応を利用したFrgment-Based Drug Discovery (2018)
  • Author(s): 高倉陽平、真鍋良幸、深瀬浩一
  • Organizer: 第98回日本化学年会
• [Presentation] 酵素反応による糖鎖伸長を鍵とするポリラクトサミン含有多分枝N-結合型糖鎖の合成 (2018)
  • Author(s): 岡村壮一郎，真鍋良幸，初村洋紀，Hsin-Ru Wu，Chun-Cheng Lin，深瀬浩一
  • Organizer: 第98回日本化学年会
• [Presentation] Efficient Synthesis of alpha-gal Epitope and Development of Novel Immunoteraphy Using the Conjugate of Anti-cancer Antibody with a-gal Epitope (2017)
  • Author(s): Julinton Sianturi, Yoshiyuki Manabe, Kento Tokunaga, Kazuya Kabayama, Koichi Fukase
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] 多様性志向型合成戦略を用いた FUT8 阻害剤の開発 (2017)
  • Author(s): 髙倉陽平、真鍋良幸、笠原里実，深瀬 浩一
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] 効率的α-マンノシル化を可能とする保護基パターンの探索と複合型N-グリカンの合成 (2017)
  • Author(s): 初村洋紀、真鍋良幸、岡村壮一郎、深瀬浩一
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] ポリラクトサミン鎖含有多分枝N-グリカンの化学酵素合成研究 (2017)
  • Author(s): 岡村 壮一郎、初村 洋紀、真鍋 良幸、深瀬 浩一、呉 心如、林 俊成
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] Synthesis and immunological evaluation of self-adjuvanting N-acetyl and N-propionyl clustered Sialyl-Tn conjugate as anticancer vaccine candidate (2017)
  • Author(s): Tsung-Che Chang, Yoshiyuki Manabe, Yukari Fujimoto, Shino Ohshima, Yoshie Kametani, Kazuya Kabayama, Yuka Nimura, Chun-Cheng Lin, Koichi Fukase
  • Organizer: 2nd ISBC symposium
  • Int'l Joint Research
• [Presentation] Chemoenzymatic synthesis of multi-antennary N-glycans containing polylactosamine (2017)
  • Author(s): Soichiro Okamura, Yoshiyuki Manabe, Hiroki Shomura, Hsin-Ru Wu, Chun-Cheng Lin, Koichi Fukase
  • Organizer: 11th International Symposium on Integrated Synthesis (ISONIS-11)&3rd International Symposium on Middle Molecular Strategy (ISMMS-3)
  • Int'l Joint Research
• [Presentation] Development of FUT8 inhibitors based on diversity-oriented synthesis (2017)
  • Author(s): Yohei Takakura, Yoshiyuki Manabe, Satomi Kasahara, Koichi Fukase
  • Organizer: 11th International Symposium on Integrated Synthesis (ISONIS-11)&3rd International Symposium on Middle Molecular Strategy (ISMMS-3)
  • Int'l Joint Research
• [Presentation] Reconstructing glycan environments using synthesized glycans (2017)
  • Author(s): Yoshiyuki Manabe, Shuto Miyake, Hiroaki Shomura, Masahiro Nagasaki, Hiroki Ichikawa, Kazuya Kabayama, Koichi Fukase
  • Organizer: 6th Gratama Workshop Groningen
  • Int'l Joint Research / Invited
• [Presentation] Chemical syntheses of N-glycans for their functional analysis (2017)
  • Author(s): Yoshiyuki Manabe, Koichi Fukase
  • Organizer: Naposaka workshop
  • Int'l Joint Research / Invited
• [Presentation] Novel approaches for cancer immunotherapy using α-gal epitope (2017)
  • Author(s): Yoshiyuki Manabe, Hao-Sheng Li, Julinton Sianturi, Naoko Terao, Shinji Takamatsu, Masahiro Tanemura, Eiji, Miyoshi, Koichi Fukase
  • Organizer: Eurocarb19
  • Int'l Joint Research
• [Presentation] Synthesis and immunological evaluation of self-adjuvanting N-modified clustered sialyl-Tn conjugate as anticancer vaccine candidate (2017)
  • Author(s): Tsung-che Chang, Yoshiyuki Manabe, Yukari Fujimoto, Yoshie Kametani, Kazuya Kabayama, Koichi Fukase
  • Organizer: Eurocarb19
  • Int'l Joint Research
• [Presentation] 糖鎖機能解析に向けた細胞膜への合成糖鎖提示システムの開発 (2017)
  • Author(s): 三宅秀斗・真鍋良幸・初村洋紀・山地俊之・花田賢太郎・樺山一哉・深瀬浩一
  • Organizer: 第7回CSJ化学フェスタ2017
• [Presentation] 化学酵素合成法によるポリラクトサミン鎖含有多分枝N-グリカンの効率的合成研究 (2017)
  • Author(s): 岡村壮一郎，真鍋良幸，初村洋紀，Hsin-Ru Wu，Chun-Cheng Lin，深瀬浩一
  • Organizer: 第7回CSJ化学フェスタ2017
• [Presentation] FUT8 阻害剤の多様性指向型合成 (2017)
  • Author(s): 髙倉陽平・真鍋良幸・笠原里実・深瀬浩一
  • Organizer: 第7回CSJ化学フェスタ2017
• [Presentation] 分子間水素結合の形成阻害に着目したABO式血液型糖鎖の合成 (2017)
  • Author(s): 筒井正斗，真鍋良幸，深瀬浩一
  • Organizer: 第34回有機合成化学セミナー
• [Presentation] 多様性指向型合成戦略による FUT8 阻害剤の創製 (2017)
  • Author(s): 髙倉陽平・真鍋良幸・笠原里実・深瀬浩一
  • Organizer: 第11回 バイオ関連化学シンポジウム
• [Presentation] 糖鎖機能解析を目指した生細胞表面への合成糖鎖修飾法の開発 (2017)
  • Author(s): 三宅秀斗、真鍋良幸、初村洋紀、山地俊之、花田賢太郎、樺山一哉、深瀬浩一
  • Organizer: 第九回光塾
• [Presentation] Chemical syntheses of N-glycans (2017)
  • Author(s): Yoshiyuki Manabe, Koichi Fukase
  • Organizer: Academia Sinica & Osaka University Bilateral Workshop
  • Place of Presentation: Taipei（Taiwan）
  • Int'l Joint Research / Invited
• [Presentation] Development of the Novel Cancer Immunotherapy Utilizing & alpha-Gal (2017)
  • Author(s): Yoshiyuki Manabe, Koichi Fukase
  • Organizer: 第7回 日中若手化学者フォーラム 7th CCS-CSJ Joint Forum on Frontier in Organic Synthesis toward Middle Molecular Strategy
  • Place of Presentation: 横浜
  • Int'l Joint Research / Invited
• [Presentation] Development of new immunotherapy using the conjugates of anti-cancer antibody with alpha-gal epitope (2017)
  • Author(s): Julinton Sianturi, Yoshiyuki Manabe, Kazuya Kabayama, Koichi Fukase
  • Organizer: Workshop for Glycoscience: Biological Function of Glycans and Lipids
  • Place of Presentation: 豊中
  • Int'l Joint Research
• [Presentation] マイクロフロー系を利用した糖鎖合成 (2017)
  • Author(s): 真鍋良幸
  • Organizer: コンソーシアム講演会「マイクロフロー合成システムの新展開（糖鎖合成、光触媒、電場と磁場）」
  • Place of Presentation: 京都
  • Invited
• [Presentation] Novel tumor immunotherapy using alpha-gal antibody conjugates (2016)
  • Author(s): Julinton Sianturi, Yoshiyuki Manabe, Kento Tokunaga, Hao-Sheng Li, Kazuya Kabayama, Koichi Fukase
  • Organizer: 4th Asian Chemical Biology Conference (ACBC4)
  • Place of Presentation: Kaohsiung（Taiwan）
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] Synthesis of Glycodendrimers as the Inhibitors of Pathogen's Infection (2016)
  • Author(s): Shuto Miyake, Yoshiyuki Manabe, Hiroaki Ichikawa, Kazuya Kabayama, Toshiyuki Yamaji, Kentaro Hanada, Koichi Fukase
  • Organizer: The 10th International Symposium on Integrated Synthesis (ISONIS-10)
  • Place of Presentation: 淡路
  • Year and Date: 2016-11-18
  • Int'l Joint Research
• [Presentation] Synthesis of N-glycans containing bisecting GlcNAc or polylactosamine (2016)
  • Author(s): 初村洋紀、岡村壮一郎、真鍋良幸、源直也、長崎政裕、深瀬浩一
  • Organizer: The 10th International Symposium on Integrated Synthesis (ISONIS-10)
  • Place of Presentation: 淡路
  • Year and Date: 2016-11-18
  • Int'l Joint Research
• [Presentation] Development of novel tumor immunoteraphy using the conjugate of antibody with alpha-gal epitope (2016)
  • Author(s): Julinton Sianturi, Yoshiyuki Manabe, Kazuya Kabayama, Koichi Fukase
  • Organizer: The 10th International Symposium on Integrated Synthesis (ISONIS-10)
  • Place of Presentation: 淡路
  • Year and Date: 2016-11-18
  • Int'l Joint Research
• [Presentation] Functional analysis of glycolipid and glycoprotein using synthetic glycans (2016)
  • Author(s): Kazuya Kabayama, Shuto Miyake, Yoshiyuki Manabe, Hiroki Shomura, Toshiyuki Yamaji, Kentaro Hanada, Koichi Fukase
  • Organizer: Sialoglyco 2016
  • Place of Presentation: Santa Barbara（USA）
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] FUT8阻害剤の細胞における機能評価 (2016)
  • Author(s): 武部智之・真鍋良幸・笠原里美・Yang Xiaoxiao・樺山一哉・深瀬浩一
  • Organizer: 第6回CSJ化学フェスタ2016
  • Place of Presentation: 船堀
  • Year and Date: 2016-11-14
• [Presentation] Efficient Synthesis of a-gal Epitope and Development of Novel Immunoteraphy Using the Conjugate of Anti-cancer Antibody with alpha-gal Epitope (2016)
  • Author(s): Julinton Sianturi, Yoshiyuki Manabe, Kazuya Kabayama, Koichi Fukase
  • Organizer: 第6回CSJ化学フェスタ2016
  • Place of Presentation: 船堀
  • Year and Date: 2016-11-14
• [Presentation] Preparation of the Pseudo Membrane Glycoprotein Using HaloTag Technology: Novel Approach for the Functional Analysis of the Glycan on Cel Surface. (2016)
  • Author(s): Shuto Miyake, Yoshiyuki Manabe, Hiroki Shomura, Kazuya Kabayama, Toshiyuki Yamaji, Kentaro Hanada, Koichi Fukase
  • Organizer: 第53回ペプチド討論会
  • Place of Presentation: 京都
  • Year and Date: 2016-10-26
• [Presentation] alpha-galエピトープを利用した効果的がん免疫療法の開発 (2016)
  • Author(s): 真鍋良幸，李昊晟，徳永健斗，Julinton Sianturi，寺尾尚子，高松真二，種村匡 弘，三善英知，深瀬浩一
  • Organizer: 第58回天然有機化合物討論会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-14
• [Presentation] マイクロフローシステムを用いたグリコシル化反応とマイクロフローシステムの小スケール化 (2016)
  • Author(s): 増井誠二，真鍋良幸，ジュリントン シアンツリ，徳永健斗，下山敦史，福山高英，柳 日馨，深瀬浩一
  • Organizer: 第35回日本糖質学会年会
  • Place of Presentation: 高知
  • Year and Date: 2016-09-01
• [Presentation] HaloTagテクノロジーによる生細胞への糖鎖提示とそれを用いた機能解析 (2016)
  • Author(s): 三宅秀斗、初村洋紀、樺山一哉、真鍋良幸、山地俊之、花田賢太郎、深瀬浩一
  • Organizer: 第35回日本糖質学会年会
  • Place of Presentation: 高知
  • Year and Date: 2016-09-01
• [Presentation] タンパク質翻訳後修飾糖鎖の合成研究：3分枝N-グリカン骨格の効率的構築の検討 (2016)
  • Author(s): 岡村壮一郎、真鍋良幸、深瀬浩一
  • Organizer: ペプチド夏の若手勉強会
  • Place of Presentation: 八王子
  • Year and Date: 2016-07-31
• [Presentation] Efficient synthesis of N-glycans containing core fucose or bisecting GlcNAc (2016)
  • Author(s): Yoshiyuki Manabe, Nasahiro Nagasaki, Hiroki Syomura, Naoya Minamoto, Koichi Fukase
  • Organizer: XXVIII International Carbohydrate Symposium (ICS2016)
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] Functional evaluation of membrane glycolipids using HaloTag system. (2016)
  • Author(s): K. Kabayama, S. Miyake, T. Yamaji, K. Hanada, Y. Manabe, K. Fukase
  • Organizer: XXVIII International Carbohydrate Symposium (ICS2016)
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] Development of the novel tumor immunotherapy using alpha-gal epitope as an adjuvant. (2016)
  • Author(s): Koichi Fukase, Hao Sheng Li, Yoshiyuki Manabe, Kento Tokunaga, Naoko Terao, Shinji Takamatsu, Masahiro Tanemura, Eiji MIyoshi
  • Organizer: XXVIII International Carbohydrate Symposium (ICS2016)
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] コアフコース機能調節分子の開発とそれを用いたTGF‐βシグナル制御と繊維化抑制 (2016)
  • Author(s): 武部智之, 真鍋良幸, 笠原里美, Yang Xiaoxiao, 樺山一哉, 深瀬浩一
  • Organizer: 第51回天然物化学談話会
  • Place of Presentation: 新潟
  • Year and Date: 2016-07-06
• [Presentation] Synthetic and biofunctional studies of microbial and animal glycans (2016)
  • Author(s): K. Kabayama, S. Miyake, Y. Manabe, T. Yamaji, K. Hanada, K. Fukase
  • Organizer: The 10th International Symposium on Glycosyltransferases (GlycoT2016)
  • Place of Presentation: Toronto
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] 細胞表面における糖鎖機能解明を指向した合成糖鎖の細胞膜提示システムの開発 (2016)
  • Author(s): 三宅秀斗、樺山一哉、真鍋良幸、陳思宇、山地俊之、花田賢太郎、深瀬浩一
  • Organizer: 日本ケミカルバイオロジー学会
  • Place of Presentation: 京都
  • Year and Date: 2016-06-15
• [Presentation] 合成糖鎖の細胞膜提示システムによる糖脂質機能の解明 (2016)
  • Author(s): 樺山一哉、三宅秀斗、真鍋良幸、山地俊之、花田賢太郎、深瀬浩一
  • Organizer: 第 58 回 日本脂質生化学会
  • Place of Presentation: 秋田
  • Year and Date: 2016-06-09
• [Presentation] Synthetic Study of Immunoconjugates Composed of Tumor Antigens and Adjuvants. (2016)
  • Author(s): Koichi Fukase, Yoshiyuki Manabe, Tsung-Che Chang, Hao Sheng Li, Yukari Fujimoto, Naoko Terao, Shinji Takamatsu, Masahiro Tanemura, Eiji Miyoshi
  • Organizer: The 16th Akabori Conference 2016 (Japanese-German Symposium on Peptide Science)
  • Place of Presentation: Kobe
  • Year and Date: 2016-05-22
  • Int'l Joint Research / Invited
• [Presentation] Functional evaluation of membrane glycolipids using synthetic glycans (2016)
  • Author(s): K. Kabayama, S. Miyake, Y. Manabe, T. Yamaji, K. Hanada, K. Fukase
  • Organizer: The 1st Korea-Japan Lipid Biology symposium
  • Place of Presentation: Jeju（Korea）
  • Year and Date: 2016-05-11
  • Int'l Joint Research
• [Presentation] Functional analysis of synthetic glycans on the cell membrane by input control (2016)
  • Author(s): K. Kabayama, S. Miyake, Y. Manabe, T. Yamaji, K. Hanada, K. Fukase
  • Organizer: Frontiers in Sialic acid Research Conference
  • Place of Presentation: Bad Lauterberg（Germany）
  • Year and Date: 2016-04-23
  • Int'l Joint Research
• [Presentation] Glycosylation via Short Time Intermediate Using Small Scale Microfluidic System. (2016)
  • Author(s): Seiji Masui, Yoshiyuki Manabe, Sianturi Julinton, Kento Tokunaga, Koichi Fukase
  • Organizer: The 12th International Symposium on Organic Reactions（ISOR-12）
  • Place of Presentation: Kyoto
  • Year and Date: 2016-04-22
  • Int'l Joint Research
• [Presentation] Development of the small-scale microfluidic system and investigation of efficient glycosylations under microfluidic condition (2016)
  • Author(s): Seiji Masui, Yoshiyuki Manabe, Sianturi Julinton, Kento Tokunaga, Atsushi Shimoyama, Takahide Fukuyama, Ilhyong Ryu and Koichi Fukase
  • Organizer: Shanghai Institute of Organic Chemistry - Osaka University Workshop on Glycoscience
  • Place of Presentation: 豊中
  • Int'l Joint Research
• [Presentation] Middle Molecular Strategy for Efficient Tumor Immunotherapy: Conjugation of Antigens with Adjuvants (2016)
  • Author(s): The 2nd International Symposium on Middle Molecular Strategy (ISMMS-2)
  • Organizer: Yoshiyuki Manabe, Koichi Fukase
  • Place of Presentation: 豊中
  • Int'l Joint Research / Invited
• [Presentation] Development of the novel tumor immunotherapy: conjugation of tumor antigens with adjuvants. (2016)
  • Author(s): Yoshiyuki Manabe, Koichi Fukase
  • Organizer: タンパク研セミナー：Frontiers of peptide and glycoscience
  • Place of Presentation: Suita
  • Int'l Joint Research / Invited
• [Presentation] FUT8阻害剤の創製-多様性指向型合成法の開拓High Throughput Screeningによる探索 (2016)
  • Author(s): 真鍋良幸、笠原里実、高倉陽平、深瀬浩一
  • Organizer: 第3回FCCAシンポジウム FCCAグライコサイエンス若手フォーラム2016
  • Place of Presentation: 湘南
• [Presentation] コアフコースの機能制御分子の創製：コアフコース認識ペプチドとFUT8阻害剤の探索 (2016)
  • Author(s): 髙倉陽平，真鍋良幸，笠原里美，Yang Xiaoxiao，奥村江里香，深瀬浩一
  • Organizer: 第3回FCCAシンポジウム FCCAグライコサイエンス若手フォーラム2016
  • Place of Presentation: 湘南
• [Presentation] 収束的ルートによる分枝構造形成を鍵とするバイセクティング型糖鎖の合成 (2016)
  • Author(s): 初村洋紀、真鍋良幸、源直也、長崎政裕、深瀬浩一
  • Organizer: 第３６回有機合成若手セミナー
  • Place of Presentation: 京都
• [Presentation] HloTagを用いた細胞表層糖鎖提示システムの開発と機能解析 (2016)
  • Author(s): 三宅秀斗、初村洋紀、樺山一哉、真鍋良幸、山地俊之、花田賢太郎、深瀬浩一
  • Organizer: 第３６回有機合成若手セミナー
  • Place of Presentation: 京都
• [Presentation] 収束的ルートによる3分枝N-グリカンの合成研究 (2016)
  • Author(s): 岡村壮一郎、真鍋良幸、深瀬浩一
  • Organizer: 第３６回有機合成若手セミナー
  • Place of Presentation: 京都
• [Book] [1]ペプチド医薬品のスクリーニング・安定化・製剤化技術 第10章6節 セルフアジュバンティングストラテジーによる合成ワクチン開発 (2017)
  • Author(s): 下山敦史，真鍋良幸，深瀬浩一
  • Total Pages: 8
  • Publisher: 技術情報協会
  • ISBN: 4861046874