| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
|
|---|
| Outline of Final Research Achievements |
Asparagine-linked oligosaccharides (N-glycans) on glycoproteins have high diversity and complexity and are involved in a variety of important physiological events. We achieved the synthesis of several complex type N-glycans, including core fucos containing N-glycan, bisecting GlcNAc containing N-glycan, and poly lactosamine containing N-glycans. We carried out live cell imaging using HaloTag to elucidate the function of N-glycans on membrane proteins. We prepared HaloTag-expressing cells and presented synthetic glycans. Then, we succesfully demonstrated that the interaction between glycans and lectins can regulate the dynamics of membrane proteins. Furthermore, we discovered the lectin recognizing core fucose. In addition, we also developed effective cancer vaccines with focusing on the relationship between glycan and immunity.
|
