Development of Microliquid Environmental Cells for X-ray Laser Diffraction Imaging

• Project/Area Number: 16H05989
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Quantum beam science
• Research Institution: The University of Tokyo (2018-2019); Hokkaido University (2016-2017)
• Principal Investigator: Kimura Takashi 東京大学, 大学院工学系研究科(工学部), 特任助教 (50531472)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000); Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2016: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
• Keywords: X線光学 / X線自由電子レーザー / マイクロ流路 / コヒーレント回折イメージング / X線ミラー / X線イメージング / X線 / 小角散乱

Outline of Final Research Achievements

We developed microfluidic environmental cells for femtosecond coherent diffraction imaging, which can observe sample imaging in solution. We proposed and developed a novel design of a microfluidic device for destructive X-ray free-electron laser (XFEL) measurement and performed a demonstration experiment at the Japanese XFEL facility, SACLA. The measured sample for the demonstration was the mixture of gold nanoparticles with two different diameters dispersed in solution. Each gold nanoparticles were modified with small molecules electrically complementary to each other. We obtained the reconstructed image of the assembly of the gold nanoparticles at about sub-10-nm resolution by numerical phase recovery from a measured diffraction pattern. The reconstruction demonstrates the successful imaging of the gold nanoparticle assembly in the microfluidic environmental cell.

Academic Significance and Societal Importance of the Research Achievements

従来の顕微イメージング技術によりナノサイズ試料を液中観察する際には、様々な困難が存在する。例えば一般的に、光学顕微鏡では可視光を利用しているために波長による分解能の制限があり、電子顕微鏡では乾燥や染色といった前処理の必要性や電子線による試料の損傷が伴う。X線自由電子レーザーとマイクロ流体デバイスを組み合わせるイメージングの可能性を開拓する本研究成果により、こうした従来手法による問題点を克服した新たな液中試料観察ツールを提供可能になり、化学や生物学や様々な領域においてこれまでにない新たな知見を得る端緒になる。

Research Products

• [Int'l Joint Research] Academia Sinica(台湾)(その他の国・地域)
• [Int'l Joint Research] 台湾中央研究院(その他の国・地域（台湾）)
• [Int'l Joint Research] 中央研究院(台湾)
• [Int'l Joint Research] 台湾中央研究院(台湾)
• [Journal Article] Design of a liquid cell toward three-dimensional imaging of unidirectionally-aligned particles in solution using X-ray free-electron lasers (2020)
  • Author(s): Suzuki Akihiro、Kimura Takashi、Yang Ying、Niida Yoshiya、Nishioka Akiko、Tachibana Tatsuro、Takei Masashi、Tono Kensuke、Yabashi Makina、Ishikawa Tetsuya、Oshima Tairo、Bessho Yoshitaka、Joti Yasumasa、Nishino Yoshinori
  • Journal Title: Physical Chemistry Chemical Physics Volume: 22 Issue: 5 Pages: 2622-2628
  • DOI: 10.1039/c9cp03658j
  • NAID: 120006957264
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] XFEL coherent diffraction imaging for weakly scattering particles using heterodyne interference (2020)
  • Author(s): C. F. Huang, W. H. Chang, T. K. Lee, Y. Joti, Y. Nishino, T. Kimura, A. Suzuki, Y. Bessho, T. T. Lee, M. C. Chen, S. M. Yang, Y. K. Hwu, S. H. Huang, P. N. Li, P. Chen, Y. C. Tseng, C. Ma, T. L. Hsu, C. H. Wong, K. Tono, T. Ishikawa, and K. S. Liang
  • Journal Title: AIP Advances Volume: 10 Issue: 5 Pages: 055219-055219
  • DOI: 10.1063/1.5129406
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Micro-liquid enclosure array and its semi-automated assembling system for x-ray free-electron laser diffractive imaging of samples in solution (2020)
  • Author(s): Takashi Kimura, Akihiro Suzuki, Ying Yang, Yoshiya Niida, Akiko Nishioka, Masashi Takei, Jinjian Wei, Hideyuki Mitomo, Yasutaka Matsuo, Kenichi Niikura, Kuniharu Ijiro, Kensuke Tono, Makina Yabashi, Tetsuya Ishikawa, Tairo Oshima, Yoshitaka Bessho Yasumasa Joti, and Yoshinori Nishino
  • Journal Title: Review of Scientific Instruments Volume: 91 Issue: 8 Pages: 083706-083706
  • DOI: 10.1063/5.0008398
  • NAID: 120007053062
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SACLAを用いた環境制御ナノイメージング (2017)
  • Author(s): 西野吉則、木村隆志、鈴木明大、城地保昌、別所義隆
  • Journal Title: レーザー研究 Volume: 45(8) Pages: 508-512
  • NAID: 130007957391
  • Int'l Joint Research
• [Journal Article] X線自由電子レーザーによる溶液環境コヒーレント回折イメージング法の開発 (2017)
  • Author(s): 木村隆志
  • Journal Title: 放射光 Volume: 30(2) Pages: 98-101
  • Acknowledgement Compliant
• [Journal Article] Generation of apodized X-ray illumination and its application to scanning and diffraction microscopy (2017)
  • Author(s): Krishna P. Khakurel, Takashi Kimura, Hiroki Nakamori, Takumi Goto, Satoshi Matsuyama, Tomoya Sasaki, Masashi Takei, Yoshiki Kohmura, Tetsuya Ishikawa, Kazuto Yamauchi, and Yoshinori Nishino
  • Journal Title: Journal of Synchrotron Radiation Volume: 24 Issue: 1 Pages: 142-149
  • DOI: 10.1107/s1600577516017677
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] X線自由電子レーザーを用いたコヒーレント回折イメージングの高度化 (2020)
  • Author(s): 木村隆志
  • Organizer: 第33回日本放射光学会年会・放射光科学合同シンポジウム（日本、愛知）
• [Presentation] マイクロ流体デバイスを用いた液中試料X線計測技術の開発 (2020)
  • Author(s): 松本豊、江川悟、山口豪太、横前俊也、湯本博勝、小山貴久、大橋治彦、三村秀和、木村隆志
  • Organizer: 精密工学会2020年度春季大会（日本、東京）
• [Presentation] 電鋳法とホログラフィック露光を組み合わせた新規光学素子開発に関する研究 (2020)
  • Author(s): 東駿吾、横前俊也、山口豪太、三村秀和、木村隆志
  • Organizer: 精密工学会2020年度春季大会（日本、東京）
• [Presentation] コヒーレント X 線光源を利用したイメージング技術の高度化 (2019)
  • Author(s): 木村隆志
  • Organizer: 光科学技術研究会（日本、東京）
• [Presentation] New Design of Environmental Cells for X-ray Laser Diffraction Imaging of Samples in Mixed Solution (2019)
  • Author(s): Takashi Kimura
  • Organizer: X Ibero American Optics Meeting, XIII Latin American Meeting on Optics, Lasers and Applications, and Mexican Optics and Photonics Meeting 2019(Mexico, Cancun)
  • Int'l Joint Research
• [Presentation] コヒーレントX線光源を利用した新規イメージング技術の開発 (2019)
  • Author(s): 木村隆志
  • Organizer: LASORセミナー（日本、千葉）
  • Invited
• [Presentation] がん治療用pH応答金ナノ粒子のX線レーザーによる液中ダイナミクス観察 (2019)
  • Author(s): 木村円香、鈴木明大、木村隆志、三友秀之、城地保昌、別所義隆、居城邦治、西野吉則
  • Organizer: 第32回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] X線自由電子レーザーを用いたポンプ・プローブコヒーレント回折イメージング法の開発 (2019)
  • Author(s): 鈴木芳幸、鈴木明大、木村隆志、三友秀之、城地保昌、別所義隆、居城邦治、西野吉則
  • Organizer: 第32回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] X線自由電子レーザーを用いたポンプ・プローブコヒーレント回折イメージング法の開発 (2019)
  • Author(s): 橘健朗、山崎憲慈、鈴木明大、木村隆志、三友秀之、城地保昌、別所義隆、郷原一寿、西野吉則
  • Organizer: 第32回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] Radiation-Damage-Free Imaging of Solid Electrolytes for Lithium-Ion Batteries by Single-Shot Coherent Diffraction Imaging (2018)
  • Author(s): Takashi Kimura
  • Organizer: International Conference on X-ray Optics and Applications 2018
  • Int'l Joint Research / Invited
• [Presentation] High-fluence x-ray focusing system for high-resolution coherent diffraction imaging at SACLA (2018)
  • Author(s): Hirokatsu Yumoto, Takahisa Koyama, Takashi Kimura, Akihiro Suzuki, Takashi Kameshima, Yasumasa Joti, Kensuke Tono, Naoya Tani, Tatsuro Tachibana, Yusuke Konishi, Yoshitaka Bessho, Yoshinori Nishino, Makina Yabashi, Haruhiko Ohashi
  • Organizer: International Conference on X-ray Optics and Applications 2018
  • Int'l Joint Research
• [Presentation] Controlled Environment Nano-Imaging Free From Radiation Damage by X-Ray Laser Diffraction (2018)
  • Author(s): Yoshinori Nishino,Takashi Kimura, Akihiro Suzuki, Yasumasa Joti, Yoshitaka Bessho
  • Organizer: 2018 MRS Spring Meeting & Exhibit
  • Int'l Joint Research
• [Presentation] Imaging thermoresponsive gold nanoparticles in solution by X-ray laser diffraction (2018)
  • Author(s): Akihiro Suzuki, Takashi Kimura, Ryo Iida, Hideyuki Mitomo, Yasumasa Joti, Yoshitaka Bessho, Ken-ichi Niikura, Kuniharu Ijiro, Yoshinori Nishino
  • Organizer: International Conference on X-ray Optics and Applications 2018
  • Int'l Joint Research
• [Presentation] High‐fluence X‐ray focusing optics for high‐resolution coherent diffractive imaging using X‐ray free electron laser (2018)
  • Author(s): Hirokatsu Yumoto, Takahisa Koyama, Takashi Kimura, Akihiro Suzuki, Takashi Kameshima, Yasumasa Joti, Kensuke Tono, Naoya Tani, Tatsuro Tachibana, Yusuke Konishi, Yoshitaka Bessho, Yoshinori Nishino, Makina Yabashi, Haruhiko Ohashi
  • Organizer: International Conference on Synchrotron Radiation Instrumentation 2018
  • Int'l Joint Research
• [Presentation] Direct Observation of Solid Electrolyte Nanoparticle for Lithium-Ion Secondary Batteries by Single-Shot Coherent Diffractive Imaging at SACLA (2018)
  • Author(s): Takashi Kimura, Akihiro Suzuki, Yasumasa Joti, Yoshitaka, Bessho, Yoshinori Nishino
  • Organizer: Cohernece 2018
  • Int'l Joint Research
• [Presentation] Recent Advances in XFEL-Based Coherent Diffractive Imaging at SACLA (2018)
  • Author(s): Akihiro Suzuki, Takashi Kimura, Yasumasa Joti, Yoshitaka, Bessho, Yoshinori Nishino
  • Organizer: Cohernece 2018
  • Int'l Joint Research / Invited
• [Presentation] Development of Multilayer Focusing Mirror System for XFEL CDI Experiments of Biological Particles (2018)
  • Author(s): Takahisa Koyama, Hirokatsu Yumoto, Takashi Kimura, Akihiro Suzuki, Takashi Kameshima, Yasumasa Joti, Kensuke Tono, Naoya Tani, Tatsuro Tachibana, Yusuke Konishi, Yoshitaka Bessho, Yoshinori Nishino, Makina Yabashi, Haruhiko Ohashi
  • Organizer: International Conference X-Ray Microscopy 2018
  • Int'l Joint Research
• [Presentation] コヒーレント回折パターンの特徴抽出による X線自由電子レーザーイメージング高度化の試み (2018)
  • Author(s): 木村隆志、鈴木明大、城地保昌、別所義隆、西野吉則
  • Organizer: 第十回放射光学会若手研究会
• [Presentation] インクジェット技術を利用した微量試料のX線自由電子レーザーイメージング手法の開発 (2018)
  • Author(s): 木村 隆志, 鈴木 明大, 城地 保昌, 別所 義隆, 西野 吉則
  • Organizer: 第31回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] パルス状コヒーレントX線溶液散乱計測用マイクロ流路型環境セルアレイの開発 (2018)
  • Author(s): 木村隆志、鈴木明大、城地保昌、別所義隆、西野吉則
  • Organizer: 精密工学会2018年度春季大会
• [Presentation] X線自由電子レーザーによる溶液環境コヒーレント回折イメージング法の開発 (2017)
  • Author(s): 木村隆志
  • Organizer: 第30回日本放射光学会年回放射光科学合同シンポジウム
  • Place of Presentation: 神戸芸術センター (兵庫, 神戸)
  • Year and Date: 2017-01-07
  • Invited
• [Presentation] Development of Single-Shot Coherent Diffraction Imaging Technique with X-ray Free-Electron Laser for Structural Analysis of Solution Samples. (2017)
  • Author(s): Takashi Kimura, Akihiro Suzuki, Yasumasa Joti, Yoshitaka Bessho, Yoshinori Nishino
  • Organizer: SACLA User's Meeting 2017
  • Int'l Joint Research / Invited
• [Presentation] X-ray Laser Imaging of DNA-Bound Nanoparticles in Inkjet-Dispensed Mixed Solution (2017)
  • Author(s): Takashi Kimura, Akihiro Suzuki, Yasumasa Joti, Yoshitaka Bessho, Yoshinori Nishino
  • Organizer: The 18th RIES-HOKUDAI International Symposium
  • Int'l Joint Research
• [Presentation] 超微細インクジェットを利用した微量混合液体試料のX線自由電子レーザーイメージング技術の開発 (2017)
  • Author(s): 木村 隆志, 鈴木 明大, 城地 保昌, 別所 義隆, 西野 吉則
  • Organizer: 2017年度精密工学会秋季大会
• [Presentation] Development of Micrometer-sized Liquid Enclosure Chip for Imaging of Samples in Solution by Single-shot X-ray Laser Diffraction. (2017)
  • Author(s): Takashi Kimura, Akihiro Suzuki, Yasumasa Joti, Yasutaka Bessho, and Yoshinori Nishino
  • Organizer: International Conference on X-ray Optics and Applications 2017
• [Presentation] Imaging of Self-Assembled Au Nanoparticle Structure in Solution by Femtosecond X-ray Laser (2016)
  • Author(s): T. Kimura, A. Suzuki, Y. Joti, Y. Bessho, and Y. Nishino
  • Organizer: The 17th RIES-HOKUDAI INTERNATIONAL SYMPOSIUM
  • Place of Presentation: Chateraise Gateaux Kingdom Sapporo (Hokkaido, Sapporo)
  • Year and Date: 2016-12-13
  • Int'l Joint Research
• [Presentation] -ray Free-electron Laser Diffraction Imaging of Solution Sample Using Micro-Liquid Enclosure Array (2016)
  • Author(s): T. Kimura, A. Suzuki, Y. Joti, Y. Bessho, and Y. Nishino
  • Organizer: 14th Conference of the Asian Crystallographic Association
  • Place of Presentation: Hanoi (Vietnam)
  • Year and Date: 2016-12-04
  • Int'l Joint Research / Invited
• [Presentation] 液中試料ダイナミクスのフェムト秒X線レーザーによる計測の試み (2016)
  • Author(s): 木村隆志, 後藤遼平, 丸岡篤史, 大島泰郎, 城地保昌, 大島泰郎, 別所義隆, 西野吉則
  • Organizer: 第4回アライアンス若手研究交流会
  • Place of Presentation: 北海道大学フロンティア応用科学研究棟 (北海道, 札幌)
  • Year and Date: 2016-11-09
• [Presentation] Imaging Gold Nanoparticle Assembly in Solution by Femtosecond X-ray Laser (2016)
  • Author(s): T. Kimura, Y. Joti, Y. Bessho, and Y. Nishino
  • Organizer: HOKUDAI-NCTU Joint Symposium on Nano, Photo and Bio Sciences in 2016
  • Place of Presentation: Frontier Research in Applied Sciences Building (Hokkaido, Sapporo)
  • Year and Date: 2016-10-04
  • Int'l Joint Research
• [Presentation] Coherent Diffractive Imaging for Solution Samples by Femsosecond X-ray Laser (2016)
  • Author(s): T. Kimura, Y. Joti, Y. Bessho, and Y. Nishino
  • Organizer: 13th International Conference on X-ray Microscopy (XRM 2016)
  • Place of Presentation: Oxford (United Kingdom)
  • Year and Date: 2016-08-15
  • Int'l Joint Research