| Budget Amount *help |
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
In this project, miRNAs from tumor cells are recognized by DNA computation technology and the nanopores. The purpose of this study is to detect the results of recognition by the nanopore measurement combined with DNA computation. This study was originally planned as a four-year project, but it was shifted to the basic research in the fourth year because the research progressed more than as we planned initially. The following is a summary of the main results obtained during the three years of research. The nanopore measurement using microdroplets is developed in a microdevice, and we succeeded in label-free and rapid decoding of the output molecule. We detected two different miRNAs with enhanced expression from small cell lung cancer as AND gates for rapid detection. After that, we also detected five different miRNAs from cholangiocarcinoma in body fluid from cancer patients. These results were published in several publications including Analytical Chemistry in the ACS.
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