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Efficient sun-light harvesting, collecting and utilizing systems constructed by assembly of pigment-containing proteins

Research Project

Project/Area Number 16H06045
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Bio-related chemistry
Research InstitutionOsaka University

Principal Investigator

Oohora Koji  大阪大学, 工学研究科, 助教 (10631202)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2016: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Keywordsヘムタンパク質 / 超分子集合体 / 光捕集系 / エネルギー移動 / 人工光合成 / ヘムタンパク室
Outline of Final Research Achievements

In this research, artificial light harvesting systems have been constructed toward efficient artificial photosynthesis systems. As scaffolds, a series of hemoprotein assemblies have been prepared and characterized. One of the assemblies shows the micelle-like sphere structure and is inverted to artificial light harvesting system by substitution of heme chromophores with photosensitizers. This system demonstrates efficient light harvesting function. The results will contribute to development of protein-based systems for artificial photosynthesis.

Academic Significance and Societal Importance of the Research Achievements

本研究は、人工光合成の実現に向けて、光子密度の低い太陽光を利用可能な光捕集系を開発した。タンパク質を用いた人工光捕集系はこれまでにいくつか報告されているが、本系は色素がタンパク質内に精密に固定されていることが特徴である。集合化したタンパク質内に色素を精密に配列し、高い光捕集能を示す系を構築した。さらに、物質変換反応の実現に向けて、コバルトやニッケルの反応中心を新規に開発した。国内外の学会や査読付きの国際学術誌にて本成果を発表し、高く評価されている。今後これらの技術を基盤とした人工光合成系の開発が期待される。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (44 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (19 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 19 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] Universitat de Strasbourg(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Friedrich-Alexander-Universitat(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Michigan(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Supramolecular dimerization of a hexameric hemoprotein via multiple pyrene-pyrene interactions2020

    • Author(s)
      Oohora Koji、Hirayama Shota、Mashima Tsuyoshi、Hayashi Takashi
    • Journal Title

      Journal of Porphyrins and Phthalocyanines

      Volume: 24 Issue: 01n03 Pages: 259-267

    • DOI

      10.1142/s1088424619500949

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Processes of Its Formation2020

    • Author(s)
      Oohora Koji、Hirayama Shota、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Chemistry Letters

      Volume: 49 Issue: 2 Pages: 186-190

    • DOI

      10.1246/cl.190855

    • NAID

      130007795242

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System2020

    • Author(s)
      Hirayama Shota、Oohora Koji、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 142 Issue: 4 Pages: 1822-1831

    • DOI

      10.1021/jacs.9b10080

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electrochemical CO2 reduction by a cobalt bipyricorrole complex: decrease of an overpotential value derived from monoanionic ligand character of the porphyrinoid species2019

    • Author(s)
      Ogawa Ayumu、Oohora Koji、Gu Wenting、Hayashi Takashi
    • Journal Title

      Chemical Communications

      Volume: 55 Issue: 4 Pages: 493-496

    • DOI

      10.1039/c8cc08876d

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A ring-shaped hemoprotein trimer thermodynamically controlled by the supramolecular heme-heme pocket interaction2019

    • Author(s)
      Oohora Koji、Kajihara Ryota、Fujimaki Nishiki、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Chemical Communications

      Volume: 55 Issue: 11 Pages: 1544-1547

    • DOI

      10.1039/c8cc09314h

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced electron transfer within supramolecular hemoprotein co-assemblies and heterodimers containing Fe and Zn porphyrins2019

    • Author(s)
      Kajihara Ryota、Oohora Koji、Hayashi Takashi
    • Journal Title

      Journal of Inorganic Biochemistry

      Volume: 193 Pages: 42-51

    • DOI

      10.1016/j.jinorgbio.2019.01.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arginine Residues Provide a Multivalent Effect for Cellular Uptake of a Hemoprotein Assembly2019

    • Author(s)
      Oohora Koji、Kajihara Ryota、Jiromaru Misa、Kitagishi Hiroaki、Hayashi Takashi
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 3 Pages: 295-298

    • DOI

      10.1246/cl.180897

    • NAID

      130007607775

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myoglobin Reconstituted with Ni Tetradehydrocorrin as a Methane‐Generating Model of Methyl‐coenzyme M Reductase2019

    • Author(s)
      Oohora Koji、Miyazaki Yuta、Hayashi Takashi
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 58 Issue: 39 Pages: 13813-13817

    • DOI

      10.1002/anie.201907584

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light triggers molecular shuttling in rotaxanes: control over proximity and charge recombination2019

    • Author(s)
      Wolf Maximilian、Ogawa Ayumu、Bechtold Mareike、Vonesch Maxime、Wytko Jennifer A.、Oohora Koji、Campidelli St?phane、Hayashi Takashi、Guldi Dirk M.、Weiss Jean
    • Journal Title

      Chemical Science

      Volume: 10 Issue: 13 Pages: 3846-3853

    • DOI

      10.1039/c8sc05328f

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Methane generation via intraprotein C-S bond cleavage in cytochrome b562 reconstituted with nickel didehydrocorrin2019

    • Author(s)
      Miyazaki Yuta、Oohora Koji、Hayashi Takashi
    • Journal Title

      Journal of Organometallic Chemistry

      Volume: 901 Pages: 120945-120945

    • DOI

      10.1016/j.jorganchem.2019.120945

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme-Heme Exciton Coupling2018

    • Author(s)
      Oohora Koji、Fujimaki Nishiki、Kajihara Ryota、Watanabe Hiroki、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 140 Issue: 32 Pages: 10145-10148

    • DOI

      10.1021/jacs.8b06690

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Characterization of meso-Substituted Cobalt Tetradehydrocorrin and Evaluation of Its Electrocatalytic Behavior Toward CO2 Reduction and H2 Evolution2018

    • Author(s)
      Ogawa Ayumu、Oohora Koji、Hayashi Takashi
    • Journal Title

      Inorganic Chemistry

      Volume: 57 Issue: 23 Pages: 14644-14652

    • DOI

      10.1021/acs.inorgchem.8b02333

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successive energy transfer within multiple photosensitizers assembled in a hexameric hemoprotein scaffold2018

    • Author(s)
      Mashima Tsuyoshi、Oohora Koji、Hayashi Takashi
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 3200-3209

    • DOI

      10.1039/c7cp05257j

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of myoglobin reconstituted with Fe(II) oxaporphyrin: The monoanionic macrocycle provides unique cyanide binding behavior for the ferrous species2018

    • Author(s)
      Meichin Hiroyuki、Oohora Koji、Hayashi Takashi
    • Journal Title

      Inorganica Chimica Acta

      Volume: 472 Pages: 184-191

    • DOI

      10.1016/j.ica.2017.06.054

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A supramolecular assembly based on an engineered hemoprotein exhibiting a thermal stimulus-driven conversion to a new distinct supramolecular structure2017

    • Author(s)
      Oohora Koji、Onuma Yoshitaka、Tanaka Yuta、Onoda Akira、Hayashi Takashi
    • Journal Title

      Chemical Communications

      Volume: 53 Issue: 51 Pages: 6879-6882

    • DOI

      10.1039/c7cc02678a

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytic Cyclopropanation by Myoglobin Reconstituted with Iron Porphycene: Acceleration of Catalysis due to Rapid Formation of the Carbene Species2017

    • Author(s)
      K. Oohora, H. Meichin, L. Zhao, M. W. Wolf, A. Nakayama, J. Hasegawa, N. Lehnert, and T. Hayashi
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 139 Issue: 48 Pages: 17265-17268

    • DOI

      10.1021/jacs.7b10154

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Manganese(V) Porphycene Complex Responsible for Inert C-H Bond Hydroxylation in a Myoglobin Matrix2017

    • Author(s)
      Oohora Koji、Meichin Hiroyuki、Kihira Yushi、Sugimoto Hiroshi、Shiro Yoshitsugu、Hayashi Takashi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 139 Issue: 51 Pages: 18460-18463

    • DOI

      10.1021/jacs.7b11288

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Substitution of an amino acid residue axially coordinating to the heme molecule in hexameric tyrosine-coordinated hemoprotein to enhance peroxidase activity2017

    • Author(s)
      Mashima Tsuyoshi、Oohora Koji、Hayashi Takashi
    • Journal Title

      Journal of Porphyrins and Phthalocyanines

      Volume: 21 Issue: 12 Pages: 824-831

    • DOI

      10.1142/s1088424617500936

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redox Potentials of Cobalt Corrinoids with Axial Ligands Correlate with Heterolytic Co-C Bond Dissociation Energies2017

    • Author(s)
      Y. Morita, K. Oohora, A. Sawada, T. Kamachi, K. Yoshizawa, T. Hayashi
    • Journal Title

      Inorg. Chem.

      Volume: 56 (4) Issue: 4 Pages: 1950-1955

    • DOI

      10.1021/acs.inorgchem.6b02482

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Hemoproteins reconstituted with metal corrinoids as functional models of natural enzymes related to bioorganometallic species2019

    • Author(s)
      Koji Oohora
    • Organizer
      9th International Symposium on Bioorganometallic Chemistry
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Enhanced peroxidase activity of hexameric hemoprotein by substitution of an amino acid residue axially coordinating to the heme molecule2018

    • Author(s)
      真島剛史・大洞光司・林高史
    • Organizer
      BIOTECHNOLOGY AND CHEMISTRY FOR GREEN GROWTH
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-assembly of hexameric hemoprotein modified with functional molecules2018

    • Author(s)
      Shota HIRAYAMA, Koji OOHORA, Takashi HAYASHI
    • Organizer
      BIOTECHNOLOGY AND CHEMISTRY FOR GREEN GROWTH
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-assembly of hexameric hemoprotein chemically modified with functional molecules2018

    • Author(s)
      HIRAYAMA Shota; OOHORA Koji; HAYASHI Takashi
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヘムタンパク質環状六量体を基盤とする色素集積体を用いた光主訴発生触媒系の構築2018

    • Author(s)
      仲山健大、大洞光司、林高史
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] de Novo ヘムタンパク質を構成要素とするポリマーおよひゲルの構築2017

    • Author(s)
      大洞 光司、浦山 貴大、古川 泰祐、林 高史
    • Organizer
      第15回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヘムタンパク質環状六量体を基盤とする異種色素の集積化と色素間エネルギー移動2017

    • Author(s)
      真島剛史・大洞光司・林高史
    • Organizer
      第15回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Artificial protein assemblies of chemically engineered hexameric hemoprotein with functional molecules2017

    • Author(s)
      Shota Hirayama, Koji Oohora, Takashi Hayashi
    • Organizer
      14th International Symposium on Applied Bioinorganic Chemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヘムタンパク質集合体を基盤とする人工光捕集系の構築2017

    • Author(s)
      大洞 光司
    • Organizer
      第38回光化学若手の会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ヘムおよび亜鉛ポルフィリンを有するヘムタンパク質共集合体および二量体における光誘起電子移動の評価2017

    • Author(s)
      梶原 竜太・大洞 光司・林 高史
    • Organizer
      第27回金属の関与する生体関連反応シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生体材料および人工酵素を指向したヘムタンパク質の化学的改変2017

    • Author(s)
      大洞 光司
    • Organizer
      錯体化学若手の会夏の学校2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 環状六量体ヘムタンパク質を基盤とする人工光捕集系への水素発生触媒の導入2017

    • Author(s)
      仲山健大・真島剛史・大洞光司・林高史
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 柔らかなヘムタンパク質を素材とするポリマーの調製と弾性評価2017

    • Author(s)
      大洞 光司、浦山 貴大、林 高史
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヘムタンパク質の化学的改変による人工酵素・酵素モデルの開発2017

    • Author(s)
      大洞光司
    • Organizer
      ITbM/IGER Chemistry Workshop 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] De Novoヘムタンパク質を架橋ユニットとしたハイドロゲルの刺激応答性評価2017

    • Author(s)
      浦山貴大、大洞光司、林高史
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Succesive Energy Transfer in Zn-substituted Hexameric Hemoprotein with Multiple Photosensitizers as an Artificial Light Harvesting Antenna2017

    • Author(s)
      Tsuyoshi Mashima, Koji Oohora, Takashi Hayashi
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヘムタンパク質環状六量体を基盤とした異種色素を含む人工光捕集系における色素間エネルギー移動2016

    • Author(s)
      真島剛史、大洞光司、林高史
    • Organizer
      第26回金属の関与する生体関連反応シンポジウム
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヘムタンパク質の機能操作:超分子集合体および人工酵素の構築2016

    • Author(s)
      大洞光司
    • Organizer
      機能物性セミナー
    • Related Report
      2016 Annual Research Report
  • [Presentation] 共有結合で連なるヘムタンパク質ポリマーの調製とその単分子力学特性評価2016

    • Author(s)
      大洞光司、古川泰祐、浦山貴大、林高史
    • Organizer
      第10回バイオ関連シンポジウム
    • Related Report
      2016 Annual Research Report
  • [Book] “Artificial Hemoprotein Assemblies in Development of Nanobiomaterials” In Bioinspired Chemistry From Enzymes to Synthetic Models2019

    • Author(s)
      Koji Oohora, Takashi Hayashi
    • Total Pages
      18
    • Publisher
      World Scientific
    • Related Report
      2018 Annual Research Report
  • [Remarks] 大阪大学大学院工学研究科応用化学専攻林研究室

    • URL

      http://www.chem.eng.osaka-u.ac.jp/~hayashiken/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 大阪大学大学院工学研究科林研究室

    • URL

      http://www.chem.eng.osaka-u.ac.jp/~hayashiken/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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