| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2016: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
This research aims to develop a methodology for spatial dissection of biological organs at single cell resolution to realize biosampling of single cells with spatial information. The device we developed has an array of the blades of nanometer~micrometer width to cut and pixelize an organ sample. In the project, spatial dissection system have been developed as a component attached to a conventional microscope. We demonstrated spatial dissection using a mouse brain slice as a model sample, and analyse its transcriptomics information. We also developed techniques to collect dissected sample with high throughput, employing 3D fabrication of microfluidic device.
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