Direct bioproduction of useful chemicals by displaying multi-functional cellulase on its cell surface

• Project/Area Number: 16H06132
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: Tanaka Tsutomu 神戸大学, 工学研究科, 准教授 (90436551)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: バイオリファイナリー / 代謝工学 / 細胞表層工学 / 細胞表層提示 / バイオプロセス / バイオテクノロジー

Outline of Final Research Achievements

We developed useful chemicals producing microorganisms from cellulosic biomass. Based on the cell surface display techniques, we introduced beta-glucosidase on its cell surface, allowing to biomass assimilation abilities. Metabolic engineering also enabled improving productivities of L-lactic acid and 3-hydroxypropionic acid。Finally, we demonstrated direct LA and 3-HP production from cellobiose with high titer and yields.

Academic Significance and Societal Importance of the Research Achievements

低炭素社会の構築、エネルギー問題の解決に向け、再生可能な資源であるバイオマスの有効利用法の開発が急務である。代謝工学の発展とともに、新しい物質生産が可能になり、前処理バイオマスの酵素糖化技術も目覚しい発展を遂げている。しかし、バイオマス分解から物質生産までを一気に行う、といった極端な研究例が多く、本研究はこれら両者の要素技術の長所をうまく融合しようとする実例として意義がある。

Research Products

• [Journal Article] Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe (2018)
  • Author(s): Takayama Seiya、Ozaki Aiko、Konishi Rie、Otomo Chisako、Kishida Mayumi、Hirata Yuuki、Matsumoto Takuya、Tanaka Tsutomu、Kondo Akihiko
  • Journal Title: Microbial Cell Factories Volume: 17 Issue: 1 Pages: 176-176
  • DOI: 10.1186/s12934-018-1025-5
  • NAID: 120006540096
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic engineering of Schizosaccharomyces pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose (2017)
  • Author(s): Ozaki Aiko、Konishi Rie、Otomo Chisako、Kishida Mayumi、Takayama Seiya、Matsumoto Takuya、Tanaka Tsutomu、Kondo Akihiko
  • Journal Title: Metabolic Engineering Communications Volume: 5 Pages: 60-67
  • DOI: 10.1016/j.meteno.2017.08.002
  • Peer Reviewed / Open Access
• [Presentation] Cadaverine production from cellobiose using Corynebacterium glutamicum (2018)
  • Author(s): Matsuura, R. Tanaka, T., Kondo, A.
  • Organizer: The 2018 International Conference on Biotechnology and Bioengineering (8th ICBB)
  • Int'l Joint Research
• [Presentation] Cadaverine production from cellobiose using Corynebacterium glutamicum (2018)
  • Author(s): Matsuura, R. Tanaka, T., Kondo, A.
  • Organizer: The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Int'l Joint Research
• [Presentation] Metabolic engineering of Escherichia coli for production of mevalonate from cellobiose (2018)
  • Author(s): Satowa, D., Fujiwara, R., Tanaka, T., Kondo, A.
  • Organizer: The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Int'l Joint Research
• [Presentation] Production of shikimic acid from cellobiose using Corynebacterium glutamicum (2018)
  • Author(s): Sato. N., Tanaka, T., Kondo, A.
  • Organizer: The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Int'l Joint Research
• [Presentation] Corynebacterium glutamicumを用いたセロビオースからのカダベリン生産技術の開発 (2018)
  • Author(s): 松浦礼奈・ 岸田真裕美・ 田中勉・近藤昭彦
  • Organizer: 化学工学会第50回秋季大会
• [Presentation] 大腸菌を用いたセロビオースからのメバロン酸生産技術の開発 (2018)
  • Author(s): 里和大地・ 藤原良介・ 田中勉・近藤昭彦
  • Organizer: 化学工学会第50回秋季大会
• [Presentation] コリネ菌を用いたセロビオースからのシキミ酸生産技術の開発 (2018)
  • Author(s): 佐藤直樹・ 田中勉・近藤昭彦
  • Organizer: 化学工学会第50回秋季大会
• [Presentation] 分裂酵母を用いた有機酸生産技術の開発 (2018)
  • Author(s): 田中勉、高山征也、尾崎愛子、近藤昭彦
  • Organizer: 化学工学会第83年会
• [Presentation] Development of fission yeast producing hydroxy compound using genome editing. (2017)
  • Author(s): Aiko Ozaki, Rie Yamaguchi, Tsutomu Tanaka, Akihiko Kondo
  • Organizer: The 7th International Symposium of Innovative Bioproduction Kobe (iBioK), Kobe
  • Place of Presentation: 神戸大学
  • Year and Date: 2017-02-03
  • Int'l Joint Research
• [Presentation] Corynebacterium glutamicum を用いたセルロース系バイオマスからのカダベリン生産技術の開発 (2017)
  • Author(s): 松浦 礼奈, 岸田 真裕美, 平田 有希, 田中 勉, 近藤 昭彦
  • Organizer: 第69回日本生物工学会大会
• [Presentation] 分裂酵母の代謝改変株を用いた 3-ヒドロキシプロピオン酸生産 (2017)
  • Author(s): 高山 征也, 小西 理絵, 田中 勉, 近藤 昭彦
  • Organizer: 第69回日本生物工学会大会
• [Presentation] 松浦礼奈、田中勉、近藤昭彦 (2017)
  • Author(s): コリネ菌を用いたセルロース系バイオマスからのカダベリン生産技術の開発
  • Organizer: 酵素工学研究会 第7８回講演会
• [Presentation] 分裂酵母の代謝改変株を用いた3-ヒドロキシプロピオン生産 (2017)
  • Author(s): 高山征也、田中勉、近藤昭彦
  • Organizer: 酵素工学研究会 第7８回講演会
• [Presentation] Metabolic engineering of S. pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose (2017)
  • Author(s): Tsutomu Tanaka, Seiya Takayama, Aiko Ozaki, Akihiko Kondo
  • Organizer: Enzyme Engineering XXIV
  • Int'l Joint Research
• [Presentation] Metabolic engineering of S. pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose (2017)
  • Author(s): Tsutomu Tanaka, Aiko Ozaki, Seiya Takayama, and Akihiko Kondo
  • Organizer: SB7.0
  • Int'l Joint Research
• [Presentation] 分裂酵母を宿主とした有用物質生産技術の開発 (2016)
  • Author(s): 尾崎愛子, 山口理絵, 田中勉, 近藤昭彦
  • Organizer: 酵素工学研究会第76回講演会
  • Place of Presentation: 東京大学
  • Year and Date: 2016-10-07
• [Presentation] 分裂酵母におけるマロニルCoAを介した有機酸生合成経路の構築 (2016)
  • Author(s): 尾崎愛子, 山口理絵, 田中勉, 近藤昭彦
  • Organizer: 化学工学会第48回秋季大会
  • Place of Presentation: 徳島大学
  • Year and Date: 2016-09-06