| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2019: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
We performed epigenomic analysis of mouse cloned embryos to improve the efficiency and quality of somatic cell cloning by developing epigenome editing technologies. Firstly, a combination of the use of Xist KO donor cells and the removal of H3K9me3 by Kdm4d significantly improved the efficiency of mouse cloning. Next, comprehensive epigenome analyses revealed that genomic imprinting by H3K27me3 was completely lost in cloned embryos. Furthermore, we also revealed that one of the H3K27me3-imprint genes, Slc38a4, plays critical role in placental development. In summary, we successfully improved the efficiency of mouse cloning and identified candidate factors that might be responsible for placental abnormalities in clones.
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