| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Outline of Final Research Achievements |
There are more than 10 DNA polymerases encoded in eukaryotic cells and two polymerases (Polδ and Polε) replicate the bulk of parental DNA. On the other hand, some of other polymerases are less processive but tolerant replication blockage. However, we know little about how activities of these DNA polymerases are coordinated during genome replication. We first tackled this issue in fission yeast using the experimental system to examine genome-wide usage of DNA polymerases (Pu-seq). We then applied this system to C. elegans and human culture cells to investigate flexibility in the mechanism of replication during change in cell identity such as differentiation and tumorigenesis.
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