| Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Outline of Final Research Achievements |
Self-growing and -division are most fundamental feature of cell for undertaking proliferation. I am trying to reproduce this phenomenon by synthesizing lipid molecules inside the lipid membrane vesicle. In this project, I reconstructed an in vitro fatty acid (FA) synthesis system by assembling 10 kinds of purified FASII enzymes that capable to synthesize 300 micro M fatty acids from acetyl-and malonyl-CoA. Although we could synthesize de novo FA in vesicle, we found that FA inactivate FAS enzymes. Thus we redesigned our system to synthesize phospholipid through FA synthesis. The responsible enzymes, PlsX and PlsY, were synthesized inside vesicles by encapsulating cell-free system. we confirmed that so synthesized PlsXY were maintaining their activity to synthesize lysophosphatidic acid. Now, we are performing this whole reactions inside vesicles to see the vesicle morphological change. We have published one Nat. Commun. and some otehr artificial cell research papers.
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