Biochemical characterizations of the cohesin ring for understanding the establishment mechanism of sister chromatid cohesion
| Project/Area Number |
16H06160
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics (2017-2018)
Tokyo Institute of Technology (2016) |
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Principal Investigator |
MURAYAMA Yasuto 国立遺伝学研究所, 新分野創造センター, 准教授 (60531663)
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| Research Collaborator |
KODERA Noriyuki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 染色体動態 / 染色体分配 / 姉妹染色分体間接着 / SMC複合体 / コヒーシン / 試験管内再構成 / 姉妹染色体接着 / ゲノム / 蛋白質 / 生化学 / SMC 複合体 |
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| Outline of Final Research Achievements |
Cohesin is a ring-shaped, multisubunit ATPase that topologically encircles DNA. The ring complex makes vital contributions to sister chromatid cohesion and global chromosomal organization to regulate numerous chromosomal events including chromosome segregation, DNA repair and transcriptional regulations. How cohesin establishes these chromosomal structures by topological DNA embrace is poorly understood. Here we found that purified cohesin mediates DNA-DNA interactions by single-stranded DNA (ssDNA)-dependent mechanism. Our genetic analyses have also suggested that ssDNA region plays important roles for establishment of sister chromatid cohesion. This study unveiled a novel cohesin’s activity and provides mechanistic insight into establishment of sister chromatid cohesion.
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| Academic Significance and Societal Importance of the Research Achievements |
染色体分配に不可欠であることに加え、コヒーシンは間期染色体のグローバルな高次構造形成も制御し、転写制御を介して分化・発生にも関与することが次々と判明している。また、様々な染色体機能が明らかになる中、その機能異常が染色体異常疾患や不妊等と関連することが報告されている。本研究は、精製コヒーシンのDNA結合活性を直接解析することで動作機構の一端を明らかにした。得られた知見は、基礎研究への貢献に加え、長期的展望として人類の健康増進に資する研究につながると期待される。
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Structure of the cohesin loader Scc22017
Author(s)
William C. H. Chao, Yasuto Murayama, Sofia Munoz, Andrew W. Jones, Benjamin O. Wade, Andrew G. Purkiss, Xiao-Wen Hu, Aaron Borg, Ambrosius P. Snijders, Frank Uhlmann, Martin R. Singleton
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Journal Title
nature COMMUNICATIONS
Volume: -
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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