| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Outline of Final Research Achievements |
The goal of this project is to manipulate and reconstitute the deformation of mammalian tissues. As the first step, we developed a molecular tool that induces apical constriction of epithelial cells and changes their shape in a photostimulation dependent manner: we induced the expression of Shroom3 with a photoactivatable transcription factor, successfully causing apical constriction in individual MDCK (Madin-Darby Canine Kidney) cells. As the second step, we induced tissue deformation by using the developed molecular tool: we illuminated a specific area of an MDCK cell sheet, successfully observing convergent movement of cell population as well as small indentation of the cell sheet.
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