Experimental reproduction of ecological speciation caused by inter-specific hybridization and elucidation of its genetic mechanism

• Project/Area Number: 16H06178
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Kyushu University
• Principal Investigator: Matsubayashi Kei 九州大学, 基幹教育院, 助教 (60528256)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2016: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
• Keywords: 雑種種分化 / 生態的種分化 / 寄主特異性 / エコゲノミクス / 種分化 / 同倍数性雑種種分化 / 植食性テントウムシ

Outline of Final Research Achievements

In Japanese phytophagous ladybird beetles, we certified that a population of the blue cohosh specialist species in Southern Hokkaido is formed as homoploid hybrid speciation caused by hybridization between closely related thistle specialist species and generalist of blue cohosh and thistle in Hokkaido. The blue cohosh specialist species, which has been thought to be a single species has evolved independently multiple times throughout Japan archipelago. Artificial F1 hybrid offspring of the two parental species in Hokkaido could accept both parental host plants, while they demonstrated significantly reduced larval survivorships on thistle. These results indicate that the blue cohosh specialist species in Southern Hokkaido evolves avoidance against unfit thistle plant after hybridization.

Academic Significance and Societal Importance of the Research Achievements

今回、種間交雑が(1) F1以降の食性幅を変え、その後 (2)特定の食草への特殊化が進む、という2段階の過程を経て種分化が起きることが示唆された。これまで雑種種分化はF1あるいはF2における超越分離で生殖隔離が生じるとされてきたが、交雑の時点(1)では大きな生殖隔離の成立には至らず、その後の選択によって生殖隔離ができていることになる。
北海道南部のルイヨウマダラテントウが本州の同種とは独立起源のものであることが示されたことで、分類学的な同一種の中に異なる遺伝的・進化的背景を持った個体群が存在することが明らかになった。これによって保全の単位の見直しを提言することが可能になる。

Research Products

• [Int'l Joint Research] The University of Sheffield(United Kingdom)
• [Journal Article] Extremely divergent host plant acceptance with gene flow between sympatric host races of the phytophagous ladybird beetle Henosepilachna diekei (2019)
  • Author(s): Matsubayashi Kei W.、Kahono Sih、Hartini Sri、Katakura Haruo
  • Journal Title: Entomological Science Volume: 22 Issue: 4 Pages: 404-412
  • DOI: 10.1111/ens.12382
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 種のちがいを量る (2019)
  • Author(s): 松林 圭
  • Journal Title: 日本生態学会 Volume: 69 Pages: 171-182
  • NAID: 130007771843
• [Journal Article] 種の境界：進化学と生態学、分子遺伝学から種分化に迫る －序論と趣旨説明－ (2019)
  • Author(s): 山口 諒、松林 圭
  • Journal Title: 日本生態学会 Volume: 69 Pages: 145-149
  • NAID: 130007771842
• [Journal Article] Genetic divergence with ongoing gene flow is maintained by the use of different hosts in phytophagous ladybird beetles genus Henosepilachna (2017)
  • Author(s): Matsubayashi K. W.、Kohyama T. I.、Kobayashi N.、Yamasaki S.、Kuwajima M.、Katakura H.
  • Journal Title: Journal of Evolutionary Biology Volume: 30 Issue: 6 Pages: 1110-1123
  • DOI: 10.1111/jeb.13071
  • Peer Reviewed
• [Journal Article] Ecological speciation (2016)
  • Author(s): 松林 圭、藤山 直之
  • Journal Title: JAPANESE JOURNAL OF ECOLOGY Volume: 66 Issue: 3 Pages: 561-580
  • DOI: 10.18960/seitai.66.3_561
  • NAID: 130006778154
  • ISSN: 0021-5007, 2424-127X
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] やっかいなお隣さんが多様性を生み出す？ (2020)
  • Author(s): 松林 圭
  • Organizer: 日本生態学会
  • Invited
• [Presentation] 異なる寄主植物への適応で生じる雑種の適応度低下 (2018)
  • Author(s): 松林 圭、Sih Kahono、片倉 晴雄
  • Organizer: 第65回日本生態学会大会
• [Presentation] 寄主変更が生み出す多次元的ニッチ分化と生殖隔離 (2017)
  • Author(s): 松林 圭
  • Organizer: 日本生態学会
  • Place of Presentation: 早稲田大学、東京、日本
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] Adaptive radiation and evolution of isolation barriers caused by specialization to different host plants in a phytophagous ladybird beetle (2017)
  • Author(s): Kei Matsubayashi
  • Organizer: The 33rd Annual Meeting of the Society of Population Ecology
  • Int'l Joint Research / Invited
• [Book] 動物学の百科事典 (2018)
  • Author(s): 公益社団法人日本動物学会
  • Total Pages: 800
  • Publisher: 丸善出版
  • ISBN: 4621303090