Reconsideration of seed germination mechanism in wheat or barley through the oxidative window

• Project/Area Number: 16H06183
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Crop production science
• Research Institution: Kyushu University
• Principal Investigator: Ishibashi Yushi 九州大学, 農学研究院, 准教授 (50611571)
• Research Collaborator: Inoue Mari (Iwaya Mari) ; Yuasa Takashi ; Matsunaka Hitoshi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000); Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
• Keywords: 種子発芽 / 活性酸素 / 植物ホルモン / 種子休眠・発芽 / 種子休眠 / 活性酸素シグナル

Outline of Final Research Achievements

Reactive oxygen species which is one of the toxic molecules is important in seed germination of wheat and barley. In this study, we identified the ABA metabolism gene and RRGs (responsive genes of reactive oxygen species) as response genes to ROS and clarified the role of these genes in seed germination and dormancy.

Academic Significance and Societal Importance of the Research Achievements

植物にとって、種子発芽は一生の幕開けとなる神秘的な生命現象であると同時に、農作物にとって収量や品質に直結する極めて重要な農業形質でもある。本研究は、農学・植物学にとって重要な種子休眠・発芽機構に、これまで悪者とされていた活性酸素が重要な役割を担っていることを明らかにした世界的にも非常に貴重な研究成果である。

Research Products

• [Journal Article] The interrelationship between abscisic acid and reactive oxygen species plays a key role in barley seed dormancy and germination (2017)
  • Author(s): Ishibashi Y, Aoki N, Kasa S, Sakamoto M, Kai K, Tomokiyo R, Yuasa T, Iwaya-Inoue M
  • Journal Title: Frontiers in Plant Science (論文ID: 240480) Volume: 8 Pages: 275-285
  • DOI: 10.3389/fpls.2017.00275
  • Peer Reviewed / Open Access
• [Presentation] Relationship between reactive oxygen species responsive gene 1 (RRG1) and plant hormones in barley (Hordeum vulgate L.) seed germination (2017)
  • Author(s): Kodama, F. Watabe, G. Moriyama, T. Okamura, K. Kubo, S. Hamaoka, N. Iwaya-Inoue, M. Ishibashi, Y.
  • Organizer: AFELiSA 2017 (The 14th International Joint Symposium among Japan and Korea)
  • Int'l Joint Research
• [Presentation] Abscisic Acid-Catalase2-Reactive Oxygen Species Cycle Is Involved in Dormancy and Germination of Barley Seeds (2016)
  • Author(s): Gaku Watabe, Nozomi Aoki, Kyohei Kai, Reisa Tomokiyo, Norimitsu Hamaoka, Mari Iwaya-Inoue, Yushi Ishibashi
  • Organizer: International symposium of Pre-harvest sprouting in cereals
  • Place of Presentation: Murdoch University
  • Year and Date: 2016-09-19
  • Int'l Joint Research
• [Presentation] オオムギの種子休眠・発芽機構における青色光と活性酸素の関係 (2016)
  • Author(s): 渡部岳 ・森山泰智 ・久保志織 ・甲斐恭平 ・濱岡範光 ・井上眞理 ・石橋勇志
  • Organizer: 日本作物学会
  • Place of Presentation: 龍谷大学
  • Year and Date: 2016-09-10
• [Book] Survival Strategies in Extreme Cold and Desiccation: Adaptation Mechanisms and their Application (2018)
  • Author(s): Ishibashi, Y. Yuasa, T. Iwaya-Inoue, M.
  • Publisher: Springer, Tokyo