| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Outline of Final Research Achievements |
To elucidate the molecular mechanism of the self-specific recognition of S-RNase by the pollen determinant SFB to exert its cytotoxicity in the Prunus-specific S-RNase-based self-incompatibility (SI), we conducted 1) identification of the pollen-tube proteins binding to the recombinant S-RNase by mass-spectrometry, 2) investigation of the poly-ubiquitination of the SI-related proteins in the (in)compatible pollinated pistils and the in vitro cultured (in)compatible pollen tubes. As a result, candidates of the pollen tube proteins involved in exerting the S-RNase cytotoxicity and those of the SCF complexes involved in detoxification of S-RNase in compatible pollen tubes were identified. On the other hand, we could not detect the poly-ubiquitination of the SI-related proteins in both of the (in)compatible pollinated pistils and the in vitro cultured (in)compatible pollen tubes.
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