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Molecular mechanisms underlying reduced boron requirement in plants

Research Project

Project/Area Number 16H06187
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionHokkaido University

Principal Investigator

MIWA Kyoko  北海道大学, 地球環境科学研究院, 准教授 (50570587)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsホウ素 / シロイヌナズナ / 欠乏耐性 / 細胞壁 / 変異体 / 遺伝子 / 要求量 / 植物 / 変異株 / 無機栄養 / 植物細胞壁 / ペクチン質多糖 / ラムノガラクツロナンII / 遺伝学
Outline of Final Research Achievements

For sustainable food and plant biomass production, it is necessary to develop plant cultivars tolerant to mineral nutrient deficiency in soil. Boron is an essential micronutrient for plants and this research aimed to identify genes to confer low-boron tolerance through reduction of boron requirement in plants. Arabidopsis thaliana mutants were characterized, which showed reduction in boron concentration in leaves but increase in leaf expansion. It was revealed that the responsible mutations were present in the genes required for synthesis of cell wall pectin, a boron binding site, and translation process. This research successfully identified the genetic resources to reduce boron requirement and proposed molecular mechanisms underlying low-boron tolerance in plants.

Academic Significance and Societal Importance of the Research Achievements

ホウ素は植物の微量必須元素のひとつであり、細胞壁の構成成分として働く。これまで植物種間や品種間でのホウ素要求量の違いは記述されてきたが、要求量を決定する遺伝子や分子機構は明らかではなかった。本研究では、植物のホウ素要求量を低下させ、ホウ素欠乏耐性を付与する遺伝子変異を複数同定し、植物の成長に必要な栄養量を変化させる分子機構の新たな知見を得た。
また、ホウ素欠乏による農業生産被害は日本を含めて世界で報告されている。本研究の知見はホウ素欠乏耐性品種の効率的な選抜や開発につながると期待される。

Report

(5 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (24 results)

All 2021 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Presentation (22 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Georgia(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Georgia(米国)

    • Related Report
      2017 Annual Research Report
  • [Presentation] Boron-dependent translation of a boron transporter and control of boron requirement in Arabidopsis thaliana2021

    • Author(s)
      Kyoko Miwa
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Reduced function of AVP2;1 confers low-boron tolerance in Arabidopsis thaliana2020

    • Author(s)
      Amarachukwu Faith Onuh, Kyoko Miwa
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 成長促進因子であるRAPTOR1B の機能欠損は低ホウ素条件での植物の主根伸長抑制を緩和する2020

    • Author(s)
      岩佐 尚樹、三輪 京子
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Boron-dependent translation of a borate transporter BOR1 and its significance for adaptation to boron nutrient availability2020

    • Author(s)
      Kyoko Miwa
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 糖転移酵素の点変異はラムノガラクツロナンIIの減少を通じてホウ素要求量を低下させる2019

    • Author(s)
      野崎佑斗、船川寛矢、相原いづみ、三輪京子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ホウ素要求量の低下したガラクツロン酸転移酵素10変異株のペクチン質多糖の変化2018

    • Author(s)
      船川寛矢、福間健、三輪京子
    • Organizer
      日本土壌肥料学会2018年度大会(神奈川大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ホウ素要求量が低下したシロイヌナズナbor1-1 抑圧変異株#101の原因遺伝子の同定2018

    • Author(s)
      野崎佑斗、船川寛矢、相原いづみ、三輪京子
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナのガラクツロン酸転移酵素遺伝子群の変異はホウ素要求量を低下させる2018

    • Author(s)
      川瀬誠喜、三輪京子
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ペクチン合成に必須な役割を担う機能未知の膜タンパク質TMN12018

    • Author(s)
      廣口覚彦、坂本真吾、光田展隆、三輪京子
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of novel genetic mutations to reduce boron requirement in pectin in Arabidopsis thaliana2017

    • Author(s)
      Funakawa H., Fukuma K., Miwa K.
    • Organizer
      The 6th International Conference on Plant Cell Wall Biology (PCWB2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Galacturonosyltransferase mutations reduce boron requirement by maintenance of rhamnogalacturonan II crosslinking rate in Arabidopsis thaliana2017

    • Author(s)
      Funakawa H., Fukuma K., Miwa K.
    • Organizer
      Taiwan Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of low-nutrient stress tolerant plants through identification of novel genes to improve nutrient use efficiency in Arabidopsis thaliana2017

    • Author(s)
      Miwa K.
    • Organizer
      Japan-China Young Women Scientists Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ガラクツロン酸転移酵素 GAUT10変異はシロイヌナズナのホウ素要求量を低下させる2017

    • Author(s)
      船川寛矢
    • Organizer
      2017年度北海道植物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 機能未知の膜タンパク質TMN1がペクチン合成に必須である2017

    • Author(s)
      廣口覚彦、坂本真吾、光田展隆、三輪京子
    • Organizer
      第11回 細胞壁研究者ネットワーク定例研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物体内のホウ素要求量を低下させる遺伝子変異の探索2017

    • Author(s)
      船川寛矢、野崎佑斗、相原いづみ、三輪京子
    • Organizer
      日本土壌肥料学会2017年度仙台大会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] A novel strategy to identify genetic mutations to improve boron use efficiency in Arabidopsis thaliana2016

    • Author(s)
      Miwa K.
    • Organizer
      Japan-China Joint workshop on soil-microbe-plant interaction
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Galacturonosyltransferase (GAUT) mutations confer low-boron tolerance by reduction of boron requirement in Arabidopsis thaliana2016

    • Author(s)
      Funakawa H., Fukuma K., Miwa K.
    • Organizer
      Cold Spring Harbor Asia conference on Latest Advances in Plant Development & Environmental Responses
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 体内の無機栄養の要求量低下による栄養欠乏耐性の向上2016

    • Author(s)
      三輪京子
    • Organizer
      農学中手の会第2回研究集会
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナのガラクツロン酸転移酵素GAUT10変異株におけるRG-II架橋率の維持によるホウ素要求量の低下2016

    • Author(s)
      船川寛矢、三輪京子
    • Organizer
      第10回細胞壁ネットワーク定例研究会/平成28年度新学術領域若手ワークショップ
    • Related Report
      2016 Annual Research Report
  • [Presentation] 体内のホウ素利用効率が上昇した新規bor1-1抑圧変異株の解析2016

    • Author(s)
      野崎佑斗、三輪京子
    • Organizer
      第10回細胞壁ネットワーク定例研究会/平成28年度新学術領域若手
    • Related Report
      2016 Annual Research Report
  • [Presentation] ガラクツロン酸転移酵素GAUT10変異はRG-II架橋率を維持することによりシロイヌナズナのホウ素要求量を低下させる2016

    • Author(s)
      船川寛矢、三輪京子
    • Organizer
      日本土壌肥料学会2016年度佐賀大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物体内のホウ素要求量を低下させる遺伝子資源の探索2016

    • Author(s)
      三輪京子
    • Organizer
      第2回植物の栄養研究会
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2022-01-27  

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