| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
|
|---|
| Outline of Final Research Achievements |
Bacteria release membrane vesicles (MVs) from their cells that range in diameter from 40-400nm. MVs serve various functions, including the transport of virulence factors, DNA transfer, and bacterial communication. They are used as technical platforms for applications in biomedicine and nanotechnology. One of the common cargo of MVs is RNA which functions has not been understood in details. In order to gain insight of the RNA associated with MVs we sequenced the RNA isolated from MVs of several bacteria. By focusing on the accumulated mRNA in MVs, this led us to discover a gene that is involved in MV biogenesis. This gene encoding a cell wall degrading enzyme is well conserved among bacteria. We show showed that this enzyme trigger explosive cell lysis and bubbling cell death through which MVs are generated in Gram-negative and Gram-positive bacteria. The results provides new concepts in MV research.
|
