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Decoding extracellular RNA released through bacterial membrane vesicles

Research Project

Project/Area Number 16H06189
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

TOYOFUKU MASANORI  筑波大学, 生命環境系, 准教授 (30644827)

Research Collaborator Nomura Nobuhiko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsメンブレンベシクル / 細菌間相互作用 / 細胞外RNA / メンブランベシクル / 膜小胞 / 微生物 / 細胞間情報伝達 / 細胞死 / 膜小包 / プログラム細胞死 / 細胞間相互作用
Outline of Final Research Achievements

Bacteria release membrane vesicles (MVs) from their cells that range in diameter from 40-400nm. MVs serve various functions, including the transport of virulence factors, DNA transfer, and bacterial communication. They are used as technical platforms for applications in biomedicine and nanotechnology. One of the common cargo of MVs is RNA which functions has not been understood in details. In order to gain insight of the RNA associated with MVs we sequenced the RNA isolated from MVs of several bacteria. By focusing on the accumulated mRNA in MVs, this led us to discover a gene that is involved in MV biogenesis. This gene encoding a cell wall degrading enzyme is well conserved among bacteria. We show showed that this enzyme trigger explosive cell lysis and bubbling cell death through which MVs are generated in Gram-negative and Gram-positive bacteria. The results provides new concepts in MV research.

Academic Significance and Societal Importance of the Research Achievements

細胞外膜小胞は、細菌以外のほとんどの生物が産生することが明らかとなってきており、その機能や応用について注目が集まっている。細菌も50年以上前からメンブレンベシクル(MV)と呼ばれる細胞外膜小胞を形成することが分かっていたが、その形成機構については完全に明らかになっていなかった。我々は、世界で初めて細菌間で共通したMV形成機構を明らかにした。本研究により、MV研究と応用が一気に進むと期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (35 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (13 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 9 results) Book (3 results) Remarks (5 results)

  • [Int'l Joint Research] チューリッヒ大学/ETH(スイス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] チューリッヒ大学/ETH(スイス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] チューリッヒ大学/ETH(スイス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] HelmholtzZentrum(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Types and origins of bacterial membrane vesicles2019

    • Author(s)
      Toyofuku M, Nomura N, Eberl L
    • Journal Title

      Nature Reviews Microbiology

      Volume: 17 Issue: 1 Pages: 13-24

    • DOI

      10.1038/s41579-018-0112-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Antibiotics stimulate vesicles formation in Staphylococcus aureus in a phage-dependent and independent fashion and via different routes2019

    • Author(s)
      Andreoni F, Toyofuku M, Menzi C, Kalawong R, Mairpady Shambat S, Francois P, Zinkernagel AS, Eberl L
    • Journal Title

      Antimicrobial Agents and Chemotherapy

      Volume: In press Issue: 2

    • DOI

      10.1128/aac.01439-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Paracoccus denitrificans can utilize various long-chain N-acylhomoserine lactones and sequester them in membrane vesicles2018

    • Author(s)
      Morinaga K, Yamamoto T, Nomura N, Toyofuku M
    • Journal Title

      Environmental Microbiology Reports

      Volume: 10 Issue: 6 Pages: 651-654

    • DOI

      10.1111/1758-2229.12674

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 細菌が放出する膜小胞の新たな展開2018

    • Author(s)
      豊福雅典
    • Journal Title

      化学と生物

      Volume: 56 Pages: 79-80

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis2017

    • Author(s)
      M. Toyofuku, G. Carcamo-Oyarce, T. Yamamoto, F. Eisenstein, C. Hsiao, M. Kurosawa, K. Gademann, M. Pilhofer, N. Nomura, and L. Eberl
    • Journal Title

      Nature communications

      Volume: 8 Issue: 1

    • DOI

      10.1038/s41467-017-00492-w

    • NAID

      120007134777

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] What will membrane vesicles (MVs) bring to bacterial communication?2017

    • Author(s)
      Masanori Toyofuku, Nobuhiko Nomura
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 3 Pages: 185-187

    • DOI

      10.1264/jsme2.ME3203rh

    • NAID

      130006105324

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] A Versatile and Rapidly Deployable Device to Enable Spatiotemporal Observations of the Sessile Microbes and Environmental Surfaces2017

    • Author(s)
      Kiyokawa T, Usuba R, Obana N, Yokokawa M, Toyofuku M, Suzuki H, Nomura N
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 1 Pages: 88-91

    • DOI

      10.1264/jsme2.ME16161

    • NAID

      130005519591

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Membrane vesicle-mediated bacterial communication2017

    • Author(s)
      Toyofuku M, Morinaga K, Hashimoto Y, Uhl J, Shimamura H, Inaba H, Schmitt-Kopplin P, Eberl L, Nomura N
    • Journal Title

      ISME J.

      Volume: 印刷中 Issue: 6 Pages: 1504-1509

    • DOI

      10.1038/ismej.2017.13

    • NAID

      120007129099

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Biofilm formation by Paracoccus denitrificans requires a type I secretion system dependent adhesin BapA2017

    • Author(s)
      Yoshida K, Toyofuku M, Obana N, Nomura N
    • Journal Title

      FEMS Microbiol Lett.

      Volume: 364 Issue: 4

    • DOI

      10.1093/femsle/fnx029

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of the alginate production on cell-to-cell communication in Pseudomonas aeruginosa PAO12017

    • Author(s)
      Yang J, Toyofuku M, Sakai R, Nomura N
    • Journal Title

      Environ Microbiol Rep.

      Volume: 印刷中 Issue: 3 Pages: 239-249

    • DOI

      10.1111/1758-2229.12521

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] メゾスコピック微生物学の夜明け2016

    • Author(s)
      豊福雅典,森永花菜, 野村暢彦
    • Journal Title

      環境バイオテクノロジー学会誌

      Volume: 16 Pages: 23-29

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞破裂を介した細菌のバイオフィルムとベシクル形成2016

    • Author(s)
      豊福雅典,野村暢彦
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 74 Pages: 420-421

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 微生物が能動的に産生するメンブランベシクルの生合成と機能2016

    • Author(s)
      尾花望, 黒沢正治,豊福雅典, 野村暢彦
    • Journal Title

      化学と生物

      Volume: 54 Pages: 812-819

    • NAID

      130006176422

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 膜小胞を介した細菌間の情報伝達2019

    • Author(s)
      豊福雅典
    • Organizer
      化学コミュニケーションのフロンティアフロンティア第2回若手シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] バクテリアが放出する膜小胞の利用に向けて2019

    • Author(s)
      豊福雅典
    • Organizer
      放線菌学会学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] メンブレンベシクルからみえてきた細菌の多様な情報戦略2018

    • Author(s)
      豊福雅典
    • Organizer
      日本微生物生態学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Challenging the dogma of bacterial membrane vesicle formation2018

    • Author(s)
      豊福雅典
    • Organizer
      10th Asian Symposium on Microbial Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細菌のメンブレンベシクル形成機構にまつわるドグマへの挑戦2018

    • Author(s)
      豊福雅典
    • Organizer
      第28回植物細菌病談話会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 低分子化合物及び膜小胞を介した細菌間相互作用に関する研究2018

    • Author(s)
      豊福雅典
    • Organizer
      日本農芸化学会 2018年度第3回関東支部例会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] メンブレンベシクルを介した細菌間コミュニケーション2018

    • Author(s)
      豊福雅典
    • Organizer
      日本細菌学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 低分子化合物及び膜小胞を介した細菌間相互作用に関する研究2018

    • Author(s)
      豊福雅典
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 細菌によるデジタルな情報伝達!?2017

    • Author(s)
      豊福雅典,森永花菜,Leo Eberl ,野村暢彦
    • Organizer
      日本生物工学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Membrane vesicle formation through cell death2017

    • Author(s)
      Masanori Toyofuku, Nobuhiko Nomura, Leo Eberl
    • Organizer
      FEMS Microbiology Congress 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] 現代コロイド界面化学の基礎2018

    • Author(s)
      豊福雅典、野村暢彦
    • Total Pages
      2
    • Publisher
      丸善出版株式会社
    • ISBN
      4621302914
    • Related Report
      2018 Annual Research Report
  • [Book] 食と微生物の辞典2017

    • Author(s)
      豊福雅典 他 199名
    • Total Pages
      512
    • Publisher
      朝倉書店
    • ISBN
      9784254431216
    • Related Report
      2017 Annual Research Report
  • [Book] Bacterial interactions” In: Encyclopedia of Biocolloid and Biointerface Sciences2016

    • Author(s)
      Masanori Toyofuku, Youske Tashiro, Inaba Tomohiro, Nobuhiko Nomura
    • Publisher
      Wiley
    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      http://www.envr.tsukuba.ac.jp/~microbio/

    • Related Report
      2018 Annual Research Report
  • [Remarks]

    • URL

      https://naturemicrobiologycommunity.nature.com/users/22125-masanori-toyofuku/posts/20247-vesicle-formation-through-bubbling-cell-death-in-bacillus-subtilis

    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      http://www.tsukuba.ac.jp/attention-research/p201709071800.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 細菌社会の情報運び役 メンブレンベシクル

    • URL

      https://www.brh.co.jp/seimeishi/journal/095/research/1.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 筑波大学 プレスリリース

    • URL

      http://www.tsukuba.ac.jp/attention-research/p201703061024.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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