mRNA crosstalk regulates central metabolic pathway in facultative anaerobic bacteria
| Project/Area Number |
16H06190
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba (2018-2019)
Gunma University (2017)
Akita Prefectural University (2016) |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2016: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Keywords | 転写後調節 / 大腸菌 / サルモネラ / TCAサイクル / small RNA / Hfq / 発現制御 / 応用微生物 / 核酸 / バイオテクノロジー / 細菌 |
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| Outline of Final Research Achievements |
mRNA encoded proteins in the same metabolic pathway but also produces a regulatory RNA. This study revealed that the TCA cycle operon mRNA of facultative aerobic bacteria, sdhCDAB-sucABCD, processes SdhX small RNA from its 3'UTR to post-transcriptionally regulate the ackA mRNA encoding acetate kinase via direct base-pairing mechanism. In addition, SdhX specifically regulates anaerobic fumarase gene fumB and unknown gene yfbV in Salmonella, while it regulates formate dehydrogenase gene fdoG and catalase gene katG in E. coli.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、オペロンmRNAは同じ代謝経路の酵素タンパク質をコードするだけでなく、その3’UTRから機能性RNAを生成し、他の代謝経路の遺伝子発現を転写後レベルで制御することを明らかにした。したがって、セントラルドグマにおいて単に遺伝情報の仲介役と考えられていたmRNAに新しい機能を見出し、1961年にJacobとMonodによって提唱されたオペロン説の概念を拡張することができた。
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Report
(4 results)
Research Products
(18 results)
